A5084645917- Lymphatic System and Diseases
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Congenital heart defects research
- Hippo pathway signaling and YAP/TAZ
- Planarian Biology and Electrostimulation
- Physiological and biochemical adaptations
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and disorders
- Fish Ecology and Management Studies
- Sphingolipid Metabolism and Signaling
- Vascular Malformations and Hemangiomas
- RNA and protein synthesis mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Single-cell and spatial transcriptomics
- Wnt/β-catenin signaling in development and cancer
- Barrier Structure and Function Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Axon Guidance and Neuronal Signaling
- Caveolin-1 and cellular processes
- Cell Image Analysis Techniques
- Marine Invertebrate Physiology and Ecology
Weizmann Institute of Science
2015-2024
Bar-Ilan University
2016
National Institutes of Health
2006-2009
Hebrew University of Jerusalem
1998-2003
University of Cambridge
1998
Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3′ UTR cis -acting element (termed VLE), which also mediates sequence-specific binding several proteins. One protein, 69-kD RBP, associates RNA microtubules in vitro. Here we show that RBP-binding sites correlate with localization. Purification cloning RBP revealed five RNA-binding motifs: four KH one RRM domains. Surprisingly, is highly homologous zipcode protein implicated...
Abstract Improved understanding of lipoproteins, particles that transport lipids throughout the circulation, is vital to developing new treatments for dyslipidemias associated with metabolic syndrome. Apolipoproteins are a key component lipoproteins. proteins structure lipoproteins and regulate lipid metabolism through control cellular exchange. Constraints cell culture mouse models mean there need complementary model can replicate complex in vivo milieu regulates apolipoprotein lipoprotein...
Coordination between the vascular system and forming organs is essential for proper embryonic development. The vasculature expands by sprouting angiogenesis, during which tip cells form filopodia that incorporate into capillary loops. Although several molecules, such as endothelial growth factor A (Vegfa), are known to induce sprouting, mechanism terminates this process ensure neovessel stability still unknown. Sphingosine-1-phosphate receptor 1 (S1P1) has been shown mediate interaction...
In recent years, there has been increasing interest in the role of lymphatics organ repair and regeneration, due to their importance immune surveillance fluid homeostasis. Experimental approaches aimed at boosting lymphangiogenesis following myocardial infarction mice, were shown promote healing heart. Yet, mechanisms governing cardiac lymphatic growth remain unclear. Here, we identify two distinct populations hearts zebrafish mouse, one that forms through sprouting lymphangiogenesis, other...
Formation and remodeling of vascular beds are complex processes orchestrated by multiple signaling pathways. While it is well accepted that vessels a particular organ display specific features enable them to fulfill distinct functions, the embryonic origins tissue-specific vessels, as molecular mechanisms regulating their formation, poorly understood. The subintestinal plexus zebrafish embryo comprises vascularize gut, liver pancreas, such represents an ideal model investigate early steps...
Both in vivo and ex observations support the hypothesis that bone mineral formation proceeds via disordered precursor phases. The characteristics of phases are not well defined, but octacalcium phosphate-like, amorphous calcium HPO42–-enriched were detected. Here we use Raman spectroscopy high-resolution wide-angle X-ray diffraction (WAXD) to characterize map at 2 μm resolution rapidly forming tail fin bones living zebrafish larvae immediately after sacrifice, respectively. shows presence an...
The formation of new vessels requires a tight synchronization between proliferation, differentiation, and sprouting. However, how these processes are differentially activated, often by neighboring endothelial cells (ECs), remains unclear. Here, we identify cell cycle progression as regulator EC sprouting differentiation. Using transgenic zebrafish illuminating stages, show that venous lymphatic precursors sprout from the cardinal vein exclusively in G1 reveal cell-cycle arrest is induced ECs...
Allan-Herndon-Dudley syndrome (AHDS) is a severe psychomotor retardation characterized by neurological impairment and abnormal thyroid hormone (TH) levels. Mutations in the TH transporter, monocarboxylate transporter 8 (MCT8), are associated with AHDS. MCT8 knock-out mice exhibit impaired levels; however, they lack defects. Here, zebrafish mct8 gene promoter were isolated, promoter-driven transgenic lines used to show that, similar humans, primarily expressed nervous vascular systems....
Confocal Raman microspectroscopy and fluorescence imaging are two well-established methods providing functional insight into the extracellular matrix living cells tissues, respectively, down to single molecule detection. In however, coexist interact. To acquire information on this cell-matrix interaction, we developed a technique for colocalized, correlative multispectral tissue analysis by implementing high-sensitivity, wide-field confocal microscope. As proof of principle, study early...
After mid-blastula transition, populations of cells within the Xenopus embryo become motile. Using antisense morpholino oligonucleotides, we find that Vg1 RBP, an RNA-binding protein implicated in RNA localization oocytes, is required for migration forming roof plate neural tube and, subsequently, crest migration. These are properly determined but remain at their site origin. Consistent with a possible role cell movement, RBP asymmetrically localizes to extended processes migrating cells....
The developing eye receives blood supply from two vascular systems, the intraocular hyaloid system and superficial choroidal vessels. In zebrafish, a highly stereotypic simple set of vessels develops on surface prior to development origins formation this so-called have not been described. We analyzed by time-lapse imaging identified their photoconversion experiments in kdrl:Kaede transgenic embryos. show that entire is derived venous origin, surprisingly, we find has, addition its previously...
Compartment boundaries are an essential developmental mechanism throughout evolution, designated to act as organizing centers and regulate localize differently fated cells. The hindbrain serves a fascinating example for this phenomenon its early development is devoted the formation of repetitive rhombomeres their well-defined in all vertebrates. Yet, actual role remains unresolved, especially amniotes.Here, we report that chick embryo consist subset cells expressing key neural stem cell...
The neural and vascular systems share common guidance cues that have direct independent signaling effects on nerves endothelial cells. Here, we show zebrafish Netrin 1a directs Dcc-mediated axon of motoneurons this function is essential for lymphangiogenesis. Specifically, secreted by the muscle pioneers at horizontal myoseptum (HMS) required sprouting dcc-expressing rostral primary motoneuron (RoP) axons neighboring along HMS, adjacent to future trajectory parachordal chain (PAC). These are...