A5039868907- Urban Transport and Accessibility
- Transportation Planning and Optimization
- Zebrafish Biomedical Research Applications
- Transportation and Mobility Innovations
- Peroxisome Proliferator-Activated Receptors
- Urban and Freight Transport Logistics
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Urban, Neighborhood, and Segregation Studies
- Human Mobility and Location-Based Analysis
- Traffic and Road Safety
- Cancer, Lipids, and Metabolism
- Receptor Mechanisms and Signaling
- Fatty Acid Research and Health
- CRISPR and Genetic Engineering
- Cholesterol and Lipid Metabolism
- Migration, Aging, and Tourism Studies
- Lipid metabolism and disorders
- Sharing Economy and Platforms
- Mitochondrial Function and Pathology
- Economic and Environmental Valuation
- Alzheimer's disease research and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
Johns Hopkins University
2016-2025
The Scarborough Hospital
2015-2024
University of Toronto
2015-2024
Carnegie Institution for Science
2015-2024
Department of Embryology
2015-2024
University of Allahabad
2024
University of Baltimore
2024
St. George's School
2023
McMaster University
2009-2021
AIDS Committee of Toronto
2021
Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by sequence-specific knockdown. Both technologies, however, can elicit undesirable off-target effects. We have several model genes these effects in detail the zebrafish, Danio rerio. Using zebrafish embryo as a template, correct mistargeting readily discernible through direct comparison of MO-injected animals with well-studied mutants. show...
Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity rapid transport cleavage products results intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free , show normal digestive organ morphology but severely reduced...
Proteolytic processing of the beta-amyloid protein precursor (APP) is regulated by cell-surface receptors. To determine whether neurotransmitter release in response to neuronal activation regulates APP brain, we electrically depolarized superfused rat hippocampal slices and measured soluble derivatives released into superfusate. Electrical depolarization caused a rapid increase both neurotransmitters amino-terminal cleavage products. These lacked carboxyl terminus were similar those found...
Abstract Dyslipidemias are a major cause of morbidity and mortality in the world, particularly developed nations. Investigating lipid lipoprotein metabolism experimentally tractable animal models is critical step toward understanding treating human dyslipidemias. The zebrafish, well-established embryological model, emerging as notable system for studies metabolism. Here, we describe value lecithotrophic, or yolk metabolizing, stages zebrafish model studying transport. We demonstrate methods...
Abstract Improved understanding of lipoproteins, particles that transport lipids throughout the circulation, is vital to developing new treatments for dyslipidemias associated with metabolic syndrome. Apolipoproteins are a key component lipoproteins. proteins structure lipoproteins and regulate lipid metabolism through control cellular exchange. Constraints cell culture mouse models mean there need complementary model can replicate complex in vivo milieu regulates apolipoprotein lipoprotein...
The pancreaticobiliary ductal system connects the liver and pancreas to intestine. It is composed of hepatopancreatic (HPD) as well intrahepatic biliary ducts intrapancreatic ducts. Despite its physiological importance, development remains poorly understood. SRY-related transcription factor SOX9 expressed in mammalian system, but perinatal lethality Sox9 heterozygous mice makes loss-of-function analyses challenging. We turned zebrafish assess role development. first show that sox9b...
Recent advances in transportation geography demonstrate the ability to compute a metropolitan scale metric of social interaction opportunities based on time-geographic concept joint accessibility. The method we put forward this article decomposes potential (SIP) into interactions within and between groups, such as people different race, income level, occupation. This provides novel exposure, one fundamental spatial dimensions segregation. In particular, SIP is disaggregated measures...
As model organism-based research shifts from forward to reverse genetics approaches, largely due the ease of genome editing technology, a low frequency abnormal phenotypes is being observed in lines with mutations predicted lead deleterious effects on encoded protein. In zebrafish, this part explained by compensation genes redundant or similar function, often resulting additional round teleost-specific whole duplication within vertebrates. Here we offer explanations for mutant phenotypes. We...
Abstract Vertebrates transport hydrophobic triglycerides through the circulatory system by packaging them within amphipathic particles called Triglyceride-Rich Lipoproteins. Yet, it remains largely unknown how are loaded onto these particles. Mutations in Phospholipase A2 group 12B ( PLA2G12B) known to disrupt lipoprotein homeostasis, but its mechanistic role this process unclear. Here we report that PLA2G12B channels lipids lumen of endoplasmic reticulum into nascent lipoproteins. This...
By consensus, the acyl-CoA synthetase (ACS) community, with advice of human and mouse genome nomenclature committees, has revised for mammalian long-chain synthetases. ACS is family root name, genes ACSs are termed ACSL1,3-6 Acsl1,3-6, respectively. Splice variants ACSL3, -4, -5, -6 cataloged. Suggestions naming other members nonmammalian synthetases made.
The transmembrane 6 superfamily member 2 ( TM6SF2 ) loss‐of‐function variant rs58542926 is a genetic risk factor for nonalcoholic fatty liver disease and progression to fibrosis but paradoxically associated with lower levels of hepatically derived triglyceride‐rich lipoproteins. expressed predominantly in small intestine, sites lipoprotein biogenesis export. In light this, we hypothesized that may exhibit analogous effects on both intestine lipid homeostasis. To test genotyped 983 bariatric...