A5101478221- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Helicobacter pylori-related gastroenterology studies
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Neurogenesis and neuroplasticity mechanisms
- Molecular Sensors and Ion Detection
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Food Quality and Safety Studies
- Genomic variations and chromosomal abnormalities
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Genetic and Kidney Cyst Diseases
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Advanced Fluorescence Microscopy Techniques
- RNA regulation and disease
- RNA Research and Splicing
Chungnam National University
2016-2025
Pusan National University
2022-2024
Emory University
2024
National Science Museum
2020-2022
California Northstate University
2022
Bucheon University
2020
Soonchunhyang University
2013-2020
Samsung Medical Center
2018-2020
Science Museum
2020
Chosun University
2016-2018
Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression a number Notch signaling genes mouse and zebrafish suggests this pathway may play role arterial-venous cell fate determination development. We show loss embryos leads to molecular defects differentiation, including markers ectopic venous within the dorsal aorta. Conversely, we find activation repression fate. Finally,...
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe establishment of gene-knockout mice zebrafish by injection RGENs as Cas9 protein:guide RNA complexes or mRNA plus guide into one-cell-stage embryos both species. efficiently generated germline transmittable mutations up to 93% newborn with minimal toxicity. RGEN-induced mouse Prkdc gene that encodes an enzyme critical for DNA...
The Delta-Notch signaling pathway is an evolutionarily conserved intercellular mechanism essential for cell fate specification. Mind bomb 1 (Mib1) has been identified as a ubiquitin ligase that promotes the endocytosis of Delta. We now report mice lacking Mib1 die prior to embryonic day 11.5, with pan-Notch defects in somitogenesis, neurogenesis,vasculogenesis and cardiogenesis. Mib1–/–embryos exhibit reduced expression Notch target genes Hes5, Hey1, Hey2 Heyl, loss N1icd generation....
Summary Although many hypo‐pigmenting agents are currently available, the demand for novel whitening is increasing, in part due to weak effectiveness and unwanted side effects of available compounds. To screen agents, methodologies such as cell culture enzymatic assays routinely used. However, these models have disadvantages terms physiological economic relevance. In this study, we validated zebrafish a whole‐animal model phenotype‐based screening melanogenic inhibitors or stimulators. We...
Abstract Uncontrolled oxidative stress impairs bone formation and induces age-related loss in humans. The FoxO family is widely accepted to play an important role protecting diverse cells from reactive oxygen species (ROS). Activation of FoxO1, the main bone, stimulates proliferation differentiation as well inhibits apoptosis osteoblast lineage cells. Despite little known about how FoxO1 expression regulated. Meanwhile, several recent studies reported that microRNAs (miRNAs) could a by...
The initiation of primary cilium assembly entails the docking ciliary vesicles presumably derived from Golgi complex to distal end mother centriole. Distal appendages, which anchor centriole plasma membrane, are thought be involved in process. However, little is known about molecular players and mechanisms that mediate vesicle–centriole association. Here we report coiled-coil domain containing 41 (CCDC41) required for vesicles. CCDC41 specifically localizes interacts with centrosomal protein...
DYRK1A maps to the Down syndrome critical region at 21q22. Mutations in this kinase-encoding gene have been reported cause microcephaly associated with either intellectual disability or autism humans. Intellectual accompanied by was recapitulated a murine model overexpressing Dyrk1a which mimicked phenotypes. However, given embryonic lethality homozygous knockout (KO) mice, no studies could present sufficient evidence link dysfunction autism. To understand molecular mechanisms underlying and...
Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question.In this study, we investigated zebrafish larvae.A cross maze with different sleeves around each arm was used for the test (R; red, G; green, B; blue, Y; yellow).The findings showed that 5 dpf larvae preferred blue over other colors (B > R G Y).To study recognition further, tyrosinase mutants were generated using CRISPR/Cas9 system.As model oculocutaneous...
Abstract Here we report SUPPORT (statistically unbiased prediction utilizing spatiotemporal information in imaging data), a self-supervised learning method for removing Poisson–Gaussian noise voltage data. is based on the insight that pixel value data highly dependent its neighboring pixels, even when temporally adjacent frames alone do not provide useful statistical prediction. Such dependency captured and used by convolutional neural network with blind spot to accurately denoise which...
Nanoscale imaging of whole vertebrates is essential for the systematic understanding human diseases, yet this goal has not been achieved. Expansion microscopy (ExM) an attractive option accomplishing aim; however, expansion even mouse embryos at mid- and late-developmental stages, which have fewer calcified body parts than adult mice, to be demonstrated due challenges expanding tissues. Here, we introduce a state-of-the-art ExM technique, termed whole-body ExM, that utilizes cyclic...
The formation of Spemann organizer is one the most important steps in dorsoventral axis determination vertebrate development. However, whether forms autonomously or induced non-cell-autonomously controversial. In this report we have isolated a novel zebrafish homeobox gene, dharma, capable inducing ectopically. expression dharma was first detected several blastomeres at side margin soon after mid-blastula transition and continued dorsal yolk syncytial layer (YSL) under embryonic shield,...
The Notch signaling pathway is an evolutionarily conserved intercellular module essential for cell fate specification that requires endocytosis of ligands. Structurally distinct E3 ubiquitin ligases, Neuralized (Neur) and Mind bomb (Mib), cooperatively regulate the ligands in Drosophila. However, respective roles mammalian Neur1, Neur2, Mib1, Mib2, development are poorly understood.Through extensive use genetics, here we show Neur1 Neur2 double mutants Mib2(-/-) mice were viable grossly...
The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis hereditary diseases. However, despite progress understanding myelination regulation, many important questions remain unanswered. To investigate mechanisms underlying vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under control mbp...
A new small molecule probe for in vivolipid imaging, LipidGreen (compound 5), was developed. stained lipid droplets 3T3L1 cell lines and fat deposits zebrafish without apparent toxicity up to 100 μM. The utility of as a drug screening platform regulation also demonstrated live zebrafish.
Mitochondria maintain a web-shaped network in cells through balance between fusion and fission. Under certain physiological pathological conditions, this is breached, as result, change mitochondrial morphology ensues. Real-time monitoring of such significant importance for studying physiology pathology, apoptosis, aging, neurodegeneration. Numerous studies have been conducted animal cell culture systems concerning change. However, very little known to date about the real-time changes at...