A5060919119- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- Ovarian cancer diagnosis and treatment
- Microbial Inactivation Methods
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Tissue Engineering and Regenerative Medicine
- Zebrafish Biomedical Research Applications
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
- Plant Virus Research Studies
- Reproductive System and Pregnancy
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroinflammation and Neurodegeneration Mechanisms
McGill University
2014-2025
McGill University Health Centre
2010-2023
Centre For Human Genetics
2021-2023
Occupational Cancer Research Centre
2015-2021
Hospital for Sick Children
2006-2010
Tokyo University of Pharmacy and Life Sciences
2009
Mount Sinai Hospital
2003-2007
Lunenfeld-Tanenbaum Research Institute
2003-2007
Kyoto University
1998-2002
Osaka University
1994-2002
Blastocyst formation marks the segregation of first two cell lineages in mammalian preimplantation embryo: inner mass (ICM) that will form embryo proper and trophectoderm (TE) gives rise to trophoblast lineage. Commitment ICM lineage is attributed function transcription factors, Oct4 (encoded by Pou5f1) Nanog. However, a positive regulator TE fate has not been described. The T-box protein eomesodermin (Eomes) caudal-type homeodomain Cdx2 are expressed TE, both Eomes Cdx2homozygous mutant...
gp130 is a common signal transducer for the interleukin-6-related cytokines. To delineate gp130-mediated growth signal, we established series of pro-B cell lines expressing chimeric receptors composed extracellular domain granulocyte colony-stimulating factor receptor and transmembrane cytoplasmic domains gp130. The second tyrosine (from membrane) gp130, which was required phosphorylation SHP-2, its association with GRB2, activation MAP kinase, essential mitogenesis, but not anti-apoptosis....
Primitive endoderm (PE) and epiblast (EPI) are two lineages derived from the inner cell mass (ICM) of E3.5 blastocyst. Recent studies showed that EPI PE progenitors expressing lineage-specific transcriptional factors Nanog Gata6, respectively, arise progressively as ICM develops. Subsequent sorting during blastocyst maturation results in ormation morphologically distinct layers at E4.5. It is, however, unknown how initial differences between populations become established Because cells...
The extra-embryonic endoderm lineage plays a major role in the nutritive support of embryo and is required for several inductive events, such as anterior patterning blood island formation. Blastocyst-derived embryonic stem (ES) trophoblast (TS) cell lines provide good models with which to study development epiblast lineages,respectively. We describe derivation characterization that are representative third blastocyst –extra-embryonic endoderm. Extra-embryonic (XEN) can be reproducibly...
Signaling between tissues is essential to form the complex, three-dimensional organization of an embryo. Because many receptor tyrosine kinases signal through RAS-MAPK pathway, phosphorylated ERK can be used as indicator when and where signaling active during development. Using whole-mount immunohistochemistry with antibodies specific ERK1 ERK2, we analyzed location, timing, distribution, duration intensity mouse embryogenesis (5-10.5 days postcoitum). Spatial temporal domains activation...
In the mouse embryo, asymmetric divisions during 8-16 cell division generate two types, polar and apolar cells, that are allocated to outer inner positions, respectively. This outer/inner configuration is first sign of formation lineages: trophectoderm (TE) mass (ICM). Outer cells become TE give rise placenta, whereas ICM embryo proper yolk sac. Here, we analyze frequency assess relationships between polarity, nuclear position, Hippo signaling activation, pathway initiates lineage-specific...
Gab1 has structural similarities with Drosophila DOS (daughter of sevenless), which is a substrate the protein tyrosine phosphatase Corkscrew. Both and have pleckstrin homology domain residues, potential binding sites for various SH2 domain-containing adapter molecules when they are phosphorylated. We found that was phosphorylated in response to cytokines, such as interleukin-6 (IL-6), IL-3, alpha interferon (IFN-α), IFN-γ. Upon stimulation IL-6 or form complex phosphatidylinositol (PI)-3...
The first two cell lineages in the mouse, surface trophectoderm (TE) and inner mass (ICM), are morphologically distinguishable by E3.5, with outer TE forming a polarized epithelial layer enclosing apolar ICM. We show here that mouse embryos completely lacking both maternal zygotic E-cadherin (cadherin 1), normal morphology of outside cells is disrupted, but individual still initiate TE- ICM-like fates. A larger proportion than showed expression markers such as Cdx2, suggesting formation an...
Abstract The dorsal gastrula organizer plays a fundamental role in establishment of the vertebrate axis. We demonstrate that zebrafish bozozok (boz) locus is required at blastula stages for formation embryonic shield, equivalent and expression multiple organizer-specific genes. Furthermore, boz essential specification dorsoanterior structures, including notochord, prechordal mesendoderm, floor plate forebrain. report mutations disrupt homeobox gene dharma. Overexpression extraembryonic yolk...
The primitive endoderm (PE) and epiblast (EPI) are two lineages derived from the inner cell mass (ICM) of E3.5 blastocyst. Although it has been shown that FGF signaling is necessary sufficient for PE specification in ICM, unknown what mechanisms control PE/EPI proportion embryo. Because modulation alone to convert all ICM cells either or EPI, a model proposed which amount embryo controls proportion. To test this model, we reduced FGF4, major preimplantation embryo, using various genotypes...
STAT3 (signal transducer and activator of transcription 3) is a key factor mediating the signals for variety cytokines, including interleukin-6 (IL-6). The Stat3 gene itself activated by IL-6 signals. We show that region signal-transducing subunit, gp130, essential activation, also required activation gene. To elucidate mechanisms activating gene, we identified an response element (IL-6RE) in Stat3gene promoter containing both low affinity STAT3-binding cAMP-responsive (CRE). Electrophoretic...
The mouse blastocyst, at the time of implantation, has three distinct cell lineages: epiblast (EPI), trophoblast and primitive endoderm (PE). Interactions between these lineages their directional growth migration are critical for establishing initial asymmetries that result in anterior–posterior patterning embryo proper. We have re–investigated timing specification relation to differential allocation progeny first two blastomeres embryonic versus abembryonic axis blastocyst. find majority...
The formation of Spemann organizer is one the most important steps in dorsoventral axis determination vertebrate development. However, whether forms autonomously or induced non-cell-autonomously controversial. In this report we have isolated a novel zebrafish homeobox gene, dharma, capable inducing ectopically. expression dharma was first detected several blastomeres at side margin soon after mid-blastula transition and continued dorsal yolk syncytial layer (YSL) under embryonic shield,...
Translational control of gene expression plays a key role during the early phases embryonic development. Here we describe transcriptional regulator mouse stem cells (mESCs), Yin-yang 2 (YY2), that is controlled by translation inhibitors, Eukaryotic initiation factor 4E-binding proteins (4E-BPs). YY2 critical in regulating mESC functions through pluripotency factors, including Octamer-binding protein 4 (Oct4) and Estrogen-related receptor-β (Esrrb). Importantly, overexpression directs...