A5034927324- Analytical Methods in Pharmaceuticals
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Assays
- Analytical Chemistry and Chromatography
- Neurogenesis and neuroplasticity mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Nerve injury and regeneration
- Pharmacogenetics and Drug Metabolism
- Hippo pathway signaling and YAP/TAZ
- Organometallic Complex Synthesis and Catalysis
- Asymmetric Hydrogenation and Catalysis
- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Natural product bioactivities and synthesis
- Anesthesia and Sedative Agents
- Pesticide Residue Analysis and Safety
- Pharmacological Effects of Natural Compounds
- Drug Solubulity and Delivery Systems
- Metabolomics and Mass Spectrometry Studies
- Phytochemistry and Biological Activities
- Cellular transport and secretion
- Pharmacological Effects and Toxicity Studies
- Asthma and respiratory diseases
Dalhousie University
2015-2024
Jilin University
2006-2022
University of Otago
2011-2020
Dunedin Public Hospital
2012-2017
Queen Elizabeth II Health Sciences Centre
2017
Mount Sinai Hospital
2000-2007
Lunenfeld-Tanenbaum Research Institute
2000-2007
University of Toronto
1972-2007
Tufts University
2007
Charles University
2007
Nerve growth factor (NGF) is released through the constitutive secretory pathway from cells in peripheral tissues and nerves where it can act as a target-derived survival factor. In contrast, brain-derived neurotrophic (BDNF) appears to be processed regulated of brain neurons secreted an activity-dependent manner play role synaptic plasticity. To determine whether sorting differences are intrinsic neurotrophins or reflect between cell types, we compared NGF BDNF processing cultured...
Berberine is an important ingredient in a number of traditional Chinese medicines but has been shown to have poor bioavailability the dog. The aim this study was use P-glycoprotein (P-glycoprotein) inhibitors cyclosporin A, verapamil and monoclonal antibody C219 vivo vitro models intestinal absorption determine role berberine absorption. In rat recirculating perfusion model, improved 6-times by inhibitors. everted sac serosal-to-mucosal transport significantly decreased A. Ussing-type...
We have asked whether p75(NTR) may play a role in neuronal apoptosis by producing transgenic mice that express the intracellular domain within peripheral and central neurons. These animals showed profound reductions numbers of sympathetic sensory neurons as well cell loss neocortex, where there is normally little or no expression. Developmental facial motor was not observed, but induced expression adult led to increased neuron death after axotomy. Biochemical analyses suggest these effects...
This article describes an in vitro investigation of the inhibition cytochrome P450 (P450) 2C9 by a series flavonoids made up flavones (flavone, 6-hydroxyflavone, 7-hydroxyflavone, chrysin, baicalein, apigenin, luteolin, scutellarein, and wogonin) flavonols (galangin, fisetin, kaempferol, morin, quercetin). With exception flavone, all were shown to inhibit CYP2C9-mediated diclofenac 4′-hydroxylation CYP2C9 RECO system, with <i>K</i><sub>i</sub> value ≤2.2 μM. In terms mechanism inhibition,...
In this report, we have tested the hypothesis that brain-derived neurotrophic factor (BDNF) is an anterograde in CNS and focused on central noradrenergic neurons synthesize BDNF. Double-label immunocytochemistry for BDNF dopamine-β-hydroxylase (DBH), a marker neurons, demonstrated partially localized to nerve fibers terminals adult rat brain. To test functional importance of BDNF, analyzed transgenic mice carrying DBH–BDNF minigene. Increased synthesis caused elevated TrkB tyrosine kinase...
Cell polarity is critical in various cellular processes ranging from cell migration to asymmetric division and axon dendrite specification. Similarly, myelination by Schwann cells polarized, but the mechanisms involved remain unclear. Here, we show that protein Par-3 localizes asymmetrically at axon-glial junction disruption of localization, overexpression knockdown, inhibits myelination. Additionally, directly associates recruits p75 neurotrophin receptor junction, forming a complex...
The mechanism of action berberine as an antihyperglycaemic agent was investigated in the Caco-2 cell line. Berberine found to effectively inhibit activity disaccharidases cells. It also decreased sucrase after preincubation with cells for 72 hours. However gluconeogenesis and glucose consumption were not influenced. 2-Deoxyglucose transporting through monolayers by but effect statistically significant. These results suggest that is at least partly due its ability alpha-glucosidase decrease...
The mRNA encoding brain-derived neurotrophic factor (BDNF) is widely distributed in central nervous system neurons, including hippocampus and cortex. However, little known about the physiology of BDNF protein within how it processed or packaged mechanisms that control its release. In this study, we have used antibodies to monitor subcellular distribution cortical extracts from adult rats treated with kainic acid. immunoreactivity elevated rat cortex 12 h after acid treatment. enriched a...
1 Salbutamol is a β 2 ,‐adrenoceptor stimulant used clinically as racemate where the activity resides predominantly in (‐)R enantiomer with little or no attributed to (+)S enantiomer. undergoes extensive pre‐systemic metabolism and active renal excretion. The pharmacokinetics of enantiomers salbutamol have been investigated after intravenous (1.6 mg) oral (4 dosing racemic drug seven normal male volunteers. Plasma urine samples were analysed by chiral h.p.1.c. solid phase extraction. 3 ratio...
In this paper we have investigated the hypothesis that neural activity causes rapid activation of TrkB neurotrophin receptors in adult mammalian CNS. These studies demonstrate kainic acid-induced seizures led to a and transient cortex. Subcellular fractionation demonstrated these activated Trk were preferentially enriched synaptosomal membrane fraction also contained postsynaptic glutamate receptors. The fast synaptic could be duplicated isolated cortical synaptosomes with KCl, presumably as...
The intracellular signaling targets used by mammalian axon guidance receptors to organize the nervous system in vivo are unclear. Nck1 and Nck2 SH2/SH3 adaptors (collectively Nck) can couple phosphotyrosine (pTyr) signals reorganization of actin cytoskeleton therefore candidates for linking cues regulatory machinery cytoskeleton. We find that selective inactivation Nck murine causes a hopping gait defect spinal central pattern generator, which is characterized synchronous firing bilateral...
Fifty-five interns, representing 71% of the medicine, obstetrics-gynecology, surgery, and pediatric interns at one medical center for year, participated in interviews involving Schedule Affective Disorders Schizophrenia-Research Diagnostic Criteria (RDC) Family History-RDC completed symptom attitudinal scales 6-month intervals. Fifteen (27%) developed a depressive syndrome during first 6 months internship. Parental history depression high scores trait neuroticism were significantly...