A5074594797- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Animal Genetics and Reproduction
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- TGF-β signaling in diseases
- Congenital heart defects research
- RNA Research and Splicing
- Mesenchymal stem cell research
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Tissue Engineering and Regenerative Medicine
- Neurobiology and Insect Physiology Research
- Scientific Computing and Data Management
- Marine Biology and Environmental Chemistry
- Cellular Mechanics and Interactions
- Mosquito-borne diseases and control
- Pregnancy and preeclampsia studies
- Genetics, Bioinformatics, and Biomedical Research
- Viral Infections and Vectors
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
Michigan State University
2016-2025
University of Massachusetts Amherst
2020
University of Michigan
2020
Michigan United
2018
University of California, Santa Cruz
2010-2014
University of California System
2014
Discovery Centre
2007-2010
Hospital for Sick Children
2006-2010
SickKids Foundation
2006-2010
University of Wisconsin–Madison
1997-2008
Blastocyst formation marks the segregation of first two cell lineages in mammalian preimplantation embryo: inner mass (ICM) that will form embryo proper and trophectoderm (TE) gives rise to trophoblast lineage. Commitment ICM lineage is attributed function transcription factors, Oct4 (encoded by Pou5f1) Nanog. However, a positive regulator TE fate has not been described. The T-box protein eomesodermin (Eomes) caudal-type homeodomain Cdx2 are expressed TE, both Eomes Cdx2homozygous mutant...
The mouse blastocyst and stem cells derived from its tissue lineages provide a unique genetic system for examining the establishment loss of pluripotency. transcription factor Cdx2 plays central role by repressing pluripotency genes, such as Oct4, promoting extraembryonic trophoblast fate at stage. However, evidence has suggested that does not work alone in lineage. We have used bioinformatic functional genomic strategies to identify Gata3 factor. show be capable inducing embryonic driving...
Pluripotent epiblast (EPI) cells, present in the inner cell mass (ICM) of mouse blastocyst, are progenitors both embryonic stem (ES) cells and fetus. Discovering how pluripotency genes regulate fate decisions blastocyst provides a valuable way to understand is normally established. EPI specified by two consecutive decisions. The first decision segregates ICM from trophectoderm (TE), an extraembryonic type. second subdivides into primitive endoderm (PE), another Here, we investigate roles...
A unique property of the mammalian embryo is that stem cells can be derived from its early tissue lineages. These lineages will give rise to fetus as well essential extraembryonic tissues. Understanding how chromatin regulation participates in establishment these and their provides insight critically inform our understanding embryogenesis cell biology. Here, we compare genomewide location active repressive histone modifications embryonic cells, trophoblast endoderm mouse. Our results show...
Pioneer transcription factors (TFs), such as OCT4 and SOX2, play crucial roles in pluripotency regulation. However, the master TF-governed regulatory circuitry was largely inferred from cultured cells. In this work, we investigated SOX2 binding embryonic day 3.5 (E3.5) to E7.5 mouse. E3.5 inner cell mass (ICM), regulates ICM-trophectoderm program but is dispensable for opening global enhancers. Instead, occupies preaccessible enhancers part opened by early-stage expressing TFs TFAP2C NR5A2....
ABSTRACT The BMP-like signaling mediated by the ligands Dpp and Gbb is required to reinforce development of most veins in Drosophila wing. However, formation cross especially sensitive reductions signaling. We show here that definitive occurs after initial specification longitudinal a process requires localized activity. Since levels are not detectably higher early phases vein development, other factors apparently account for this Our evidence suggests product crossveinless 2 gene novel...
During mammalian development, the challenge for embryo is to override intrinsic cellular plasticity drive cells distinct fates. Here, we unveil novel roles HIPPO signaling pathway in controlling cell positioning and expression of Sox2, first marker pluripotency mouse early embryo. We show that maternal zygotic YAP1 WWTR1 repress Sox2 while promoting trophectoderm gene Cdx2 parallel. Yet, more sensitive than Yap1/Wwtr1 dosage, leading a state conflicted fate when YAP1/WWTR1 activity moderate....
The first cellular differentiation event in mouse development leads to the formation of blastocyst consisting inner cell mass (ICM) and trophectoderm (TE). transcription factor CDX2 is required for proper TE specification, where it promotes expression genes, represses Pou5f1 (OCT4). However its downstream network developing embryo not fully characterized. Here, we performed high-throughput single qPCR analysis Cdx2 null embryos identify CDX2-regulated targets vivo. To genes likely be...
ABSTRACT It is thought that the posterior expression of ‘selector’ genes engrailed and invected control subdivision growing wing imaginal disc Drosophila into anterior lineage compartments. At present, cellular mechanisms by which separate compartments are maintained not known. Most models have assumed presence or absence selector gene autonomously drives compartment-specific adhesion recognition molecules inhibit intermixing between However, our present understanding Hedgehog signalling...
The reprogramming factors OCT4, SOX2, KLF4, and MYC (OSKM) can reactivate the pluripotency network in terminally differentiated cells, but also regulate expression of non-pluripotency genes other contexts, such as mouse primitive endoderm. endoderm is an extraembryonic lineage established parallel to pluripotent epiblast blastocyst, progenitor pool for stem (XEN) cells. We show that OSKM induce endodermal genes, leading formation induced XEN (iXEN) which possess key properties...
The Drosophila tolloid (tld) and related (tlr) gene products belong to a family of developmentally important proteases that includes Bone Morphogenetic Protein 1 (Bmp1). Tld is required early in development for proper patterning dorsal embryonic structures, whereas Tlr later during larval pupal stages development. major function augment the activity Decapentaplegic (Dpp) Screw (Scw), two members Bmp subgroup Tgf beta superfamily, by cleaving inhibitor Short gastrulation (Sog). In this study,...
In many invertebrate and vertebrate species, cell fates are assigned through the cellular inheritance of differentially localized maternal determinants. Whether mammalian embryogenesis is also regulated by deterministic mechanisms highly controversial. The caudal domain transcription factor CDX2 has been reported to act as a determinant regulating fate decisions in mouse development. However, this finding contentious because reports that Cdx2 not essential for Notably, all previously...
In mice, pluripotent cells are thought to derive from buried inside the embryo around 16-cell stage. Sox2 is only pluripotency gene known be expressed specifically within at this To understand how established, we therefore investigated mechanisms regulating initial activation of expression. Surprisingly, expression initiated normally in absence both Nanog and Oct4 (Pou5f1), highlighting differences between stem cell models pluripotency. However, observed precocious, ectopic prior stage Yap1,...
TolTEC is a three-band imaging polarimeter for the Large Millimeter Telescope. Simultaneously observing with passbands at 1.1mm, 1.4mm and 2.0mm, has diffraction-limited beams FWHM of 5, 7, 11 arcsec, respectively. Over coming decade, will perform combination PI-led Open-access Legacy Survey projects. Herein we provide an overview instrument give first quantitative measures its performance in lab prior to shipping telescope 2021.
SOX15 is a broadly conserved transcription factor involved in many critical processes, including mammalian cell fate specification and pluripotency. We investigated the specificity of commercially available, polyclonal anti-SOX15 antibody advertised as knockout-validated. generated new mouse line carrying null allele Sox15 , then evaluated activity embryos. Nuclear signal was detected both wild type embryos, even when potential maternally or paternally-contributed eliminated. conclude that...