A5064949815- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Anesthesia and Sedative Agents
- Airway Management and Intubation Techniques
- Pain Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- 3D Printing in Biomedical Research
- Anesthesia and Neurotoxicity Research
- Neuropeptides and Animal Physiology
- Intensive Care Unit Cognitive Disorders
- Heart Rate Variability and Autonomic Control
- Reproductive Biology and Fertility
- Neuroscience of respiration and sleep
- Tracheal and airway disorders
- Synthesis and biological activity
- Gene Regulatory Network Analysis
- DNA Repair Mechanisms
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Atrial Fibrillation Management and Outcomes
- Tissue Engineering and Regenerative Medicine
Osaka University
2016-2025
Osaka Dental University
2013-2024
Kumamoto University
2015-2024
RIKEN Center for Computational Science
2004-2021
Japan Science and Technology Agency
2005-2016
Kobe University
2005-2015
Organogenesis (United States)
2014
Plant (United States)
2008
RIKEN Center for Biosystems Dynamics Research
2008
National Defense Medical College Hospital
2005
We present a draft genome sequence of the platypus, Ornithorhynchus anatinus. This monotreme exhibits fascinating combination reptilian and mammalian characters. For example, platypuses have coat fur adapted to an aquatic lifestyle; platypus females lactate, yet lay eggs; males are equipped with venom similar that reptiles. Analysis first aligned these features genetic innovations. find reptile proteins been co-opted independently from same gene families; milk protein genes conserved despite...
Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of late blastocyst-stage embryo to form visceral and parietal endoderm, both which have an important role in early embryogenesis. The essential roles Gata-6 Gata-4 on differentiation been identified by analyses knockout mice. Here we report that forced expression either or embryonic stem (ES) cells sufficient induce proper program towards ExE. We believe this first a physiological event induced ectopic transcription...
Embryonic stem (ES) cells are pluripotent derived from the inner cell mass (ICM) and epiblast, have been suggested to be a homogeneous population with characteristics intermediate between them. These express Oct3/4 Rex1 genes, which used as markers indicate undifferentiated state of ES cells. Whereas is expressed in totipotent mouse life cycle, expression restricted ICM, downregulated populations later stages, i.e. epiblast primitive ectoderm (PrE). To address whether comprise equivalent...
Mice with a targeted mutation of the gastric inhibitory polypeptide (GIP) receptor gene (GIPR) were generated to determine role GIP as mediator signals from gut pancreatic beta cells. GIPR-/- mice have higher blood glucose levels impaired initial insulin response after oral load. Although meal ingestion are not increased by high-fat diet in GIPR+/+ because compensatory secretion, they significantly lack such enhancement. Accordingly, early secretion mediated determines tolerance load vivo,...
Oct-3/4 is a key transcriptional factor whose expression level governs the fate of primitive inner cell mass and embryonic stem (ES) cells. Previously, an upstream 3.3-kb distal enhancer (DE) fragment was identified to be responsible for specific mouse in ES However, little known about cis-elements trans-factors required DE activity. In this study, we novel cis-element, called Site 2B here, located approximately 30 bp downstream from 2A, which previously revealed by vivo chemical...
Pituitary adenylate cyclase-activating polypeptide (PACAP) has been conserved remarkably during evolution and is widely expressed in the mammalian brain. In Drosophila, mutation of PACAP homologue results behavioral defects, including impaired olfaction-associated learning changes ethanol sensitivity. Here, we report generation mice lacking gene (PACAP(-/-)). PACAP(-/-) were born expected Mendelian ratios but had a high early-mortality rate. The surviving adult displayed remarkable changes;...
The success of Cre‐mediated conditional gene targeting depends on the specificity Cre recombinase expression in Cre‐transgenic mouse lines. As a tool to evaluate expression, we developed reporter transgenic strain that expresses enhanced green fluorescent protein (EGFP) upon recombination. We demonstrate progeny resulting from cross between this and expressing zygotes show ubiquitous EGFP fluorescence. This should be useful monitor directed by various promoters mice, including mice which is...
Transcription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, A. G. Smith, Nat. Genet. 24:372-376, 2000). Here we have developed complementation assay based on ability transgenes to rescue conditionally null ES used this which domains are required...
Although the POU transcription factor Oct3/4 is pivotal in maintaining self renewal of embryonic stem (ES) cells, little known its molecular mechanisms. We previously reported that N-terminal transactivation domain required for activation Lefty1 expression (H. Niwa, S. Masui, I. Chambers, A. G. Smith, and J. Miyazaki, Mol. Cell. Biol. 22:1526-1536, 2002). Here we test whether a direct target Oct3/4. identified an ES cell-specific enhancer upstream promoter contains binding sites Sox2. Unlike...
Large-scale gene expression profiling was performed on embryo-derived stem cell lines to identify molecular signatures of pluripotency and lineage specificity. Analysis pluripotent embryonic (ES) cells, extraembryonic-restricted trophoblast (TS) terminally-differentiated mouse embryo fibroblast (MEF) cells identified profiles unique each type, as well genes common only ES TS cells. Whereas most the MEF-specific had been characterized previously, majority (67%) ES-specific were novel did not...
Self-renewal circuitry in embryonic stem cells (ESCs) is increasingly defined. How the robust pluripotency program dissolved to enable fate transition less appreciated. Here we develop a forward genetic approach using haploid ESCs. We created libraries of transposon integrations and screened for persistent self-renewal differentiation-permissive culture. This yielded multiple mutants Fgf/Erk GSK3/Tcf3 modules known drive differentiation epigenetic modifiers implicated lineage commitment....
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated delineates the downstream target genes Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation (ChIP) assays, this analysis identifies not only primary targets Oct3/4, but also secondary or tertiary targets. Furthermore, reveals are regulated either positively negatively by Identification a group both...
Embryonic stem (ES) cells are self-renewing that maintain pluripotency to differentiate into all types of cells. Because their potential provide a variety tissues for use in regenerative medicine, there is great interest the identification growth factors govern these unique properties ES However, signaling pathways controlling cell proliferation remain largely unknown. Since transforming factor β (TGFβ) superfamily members have been implicated processes early embryogenesis, we investigated...
The transcription factor Oct4 is required in vitro for establishment and maintenance of embryonic stem cells reprogramming somatic to pluripotency. In vivo, it prevents the ectopic differentiation early embryos into trophoblast. Here, we further explore role blastocyst formation specification epiblast versus primitive endoderm lineages using conditional genetic deletion. Experiments involving mouse deficient both maternal zygotic suggest that dispensable zygote formation, cleavage activation...