A5001552909- Fusion materials and technologies
- Nuclear Materials and Properties
- Pluripotent Stem Cells Research
- Hydrogen embrittlement and corrosion behaviors in metals
- Nuclear reactor physics and engineering
- Microstructure and Mechanical Properties of Steels
- CRISPR and Genetic Engineering
- High Temperature Alloys and Creep
- High-Temperature Coating Behaviors
- Renal and related cancers
- Metal and Thin Film Mechanics
- Microstructure and mechanical properties
- Nuclear materials and radiation effects
- Ion-surface interactions and analysis
- Welding Techniques and Residual Stresses
- Metallurgy and Material Forming
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Proteoglycans and glycosaminoglycans research
- Nuclear Physics and Applications
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Thermodynamic and Structural Properties of Metals and Alloys
- Genomics and Chromatin Dynamics
Japan Atomic Energy Agency
2016-2025
Kyoto Prefectural University of Medicine
2021-2023
Tohoku University
2002-2022
RIKEN Center for Computational Science
2008-2021
Hiroshima University
2021
Japan Tobacco (Japan)
2000-2021
Kanazawa Medical University
2016-2021
Sanyo-Onoda City University
2021
Okayama University of Science
2019
Medical Research Institute
2018
Inhibition of glycogen synthase kinase-3 (Gsk3) supports mouse embryonic stem cells (ESCs) by modulating Tcf3, but the critical targets downstream Tcf3 are unclear. We analyzed intersection between genome localization and transcriptome data sets to identify genes repressed Tcf3. Among these, manipulations Esrrb gave distinctive phenotypes in functional assays. Knockdown knockout eliminated response Gsk3 inhibition, causing extinction pluripotency markers loss colony forming capability....
The N‐terminus of MDM2 proto‐oncoprotein interacts with p53 and down modulates activity by inhibiting transcriptional promoting degradation. MDMX is structurally related to also binds p53. However, the function has not been clarified yet. We found that hetero‐oligomerized through their C‐terminal RING finger domains. Yeast two‐hybrid analysis revealed hetero‐oligomerization between was more stable than homo‐oligomerization each protein. shown be degraded ubiquitin‐proteasome pathway, while a...
Various cytokines utilize Janus kinase (JAK) and the STAT (signal transducers activators of transcription) family transcription factors to carry out their biological functions. Among STATs, two highly related proteins, STAT5a STAT5b, are activated by various cytokines, including prolactin, growth hormone, erythropoietin, interleukin 2 (IL-2), IL-3. We have cloned a STAT5-dependent immediate-early cytokine-responsive gene, CIS1 (encoding cytokine-inducible SH2-containing protein 1). In this...
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated delineates the downstream target genes Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation (ChIP) assays, this analysis identifies not only primary targets Oct3/4, but also secondary or tertiary targets. Furthermore, reveals are regulated either positively negatively by Identification a group both...
Embryonic stem (ES) cells are self-renewing that maintain pluripotency to differentiate into all types of cells. Because their potential provide a variety tissues for use in regenerative medicine, there is great interest the identification growth factors govern these unique properties ES However, signaling pathways controlling cell proliferation remain largely unknown. Since transforming factor β (TGFβ) superfamily members have been implicated processes early embryogenesis, we investigated...
Sox2 is a transcription factor required for the maintenance of pluripotency. It also plays an essential role in different types multipotent stem cells, raising possibility that governs common stemness phenotype. Here we show critical downstream target fibroblast growth (FGF) signaling, which mediates self-renewal trophoblast cells (TSCs). Sustained expression together with Esrrb or Tfap2c can replace FGF dependency. By comparing genome-wide binding sites embryonic (ESCs) and TSCs combined...
Soon after fertilization, the few totipotent cells of mammalian embryos diverge to form a structure called blastocyst (BC). Although numerous cell types, including germ and extended-pluripotency stem cells, have been developed from pluripotent (PSCs) in vitro, generating functional BCs only PSCs remains elusive. Here, we describe induced self-organizing 3D BC-like cysts (iBLCs) generated mouse PSC culture. Resembling natural BCs, iBLCs blastocoel-like cavity were formed with outer expressing...
Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in cells including embryonic (ES) remained unclear although involvement visceral endoderm differentiation F9 embryonal carcinoma (EC) was reported.We showed Rex1 mouse ES well embryos using conventional gene targeting strategy. Our results clearly indicated that is dispensable both maintenance pluripotency and development embryos. Rex1-/- defect to induce subset...
Self‐renewal and differentiation of embryonic stem (ES) cells are controlled by the combinatorial action extracellular signals regulation gene expression. For characterizing entire molecular mechanism governing these events, we first established a feeder‐ serum‐free culture system in which mouse ES could propagate clonal density keeping with proper pluripotency. Supplementation peptide hormones such as adrenocorticotropic hormone (ACTH) is required to remove serum, key event this phenomenon...
Abstract Oxide-dispersion-strengthened (ODS) ferritic steel incorporating nanoscale oxide particles was produced by mechanical alloying, and the complex particles, comprised of titanium yttrium, characterized high-resolution transmission electron microscopy (HRTEM) characteristic X-ray nano-analyses. The interface between matrix major Ti-Y incoherent. From HRTEM nano-analyses, plus extremely limited literature data on ODS steels, it determined that most stable cubic Y2Ti2O7, which formed a...
Mouse epiblast stem cells (mEpiSCs) are pluripotent derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct that embryonic (mESCs) in several cell biological criteria. One the distinctions mEpiSCs contribute either not at all or much lower efficiency to chimeric embryos after blastocyst injection compared mESCs. However, here we showed can be incorporated into normal development by forced expression E-cadherin transgene for 2 days culture. Using this...
Krüppel-like factors (Klfs) have a pivotal role in maintaining self-renewal of mouse embryonic stem cells (mESCs). The functions three Klf family members (Klf2, Klf4 and Klf5) been identified, are suggested to largely overlap. For further dissection their functions, we applied an inducible knockout system for these assessed the effects combinatorial loss function. As result, confirmed that any one Klf2, Klf5 was sufficient support self-renewal, whereas removal all compromised it. activity...
The 9Cr-ODS martensitic steel claddings were developed by cold-rolling and subsequent heat-treatment. standard chemical composition is Fe-0.13C-9Cr-2W-0.2Ti-0.35Y2O3. substantially elongated grains formed turned out to be into equi-axied ferrite austenite phase transformation at the final produced have tempered structure excess oxygen of 0.060 mass%. superior tensile creep rupture strength shown in cladding, compared with conventional ferritic (PNC-FMS) even austenitic (PNC316) higher...