Woong‐Yang Park
A5003283147- Cancer Genomics and Diagnostics
- Gastric Cancer Management and Outcomes
- Gastrointestinal Tumor Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Helicobacter pylori-related gastroenterology studies
- Single-cell and spatial transcriptomics
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- RNA modifications and cancer
- BRCA gene mutations in cancer
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Multiple Myeloma Research and Treatments
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Immune cells in cancer
- Hearing, Cochlea, Tinnitus, Genetics
- Breast Cancer Treatment Studies
- Bioinformatics and Genomic Networks
- Genomics and Rare Diseases
- HER2/EGFR in Cancer Research
Samsung Medical Center
2016-2025
Sungkyunkwan University
2016-2025
Samsung (South Korea)
2016-2025
Liberty University
2025
Korea Photonics Technology Institute
2025
Seoul National University
2008-2024
Alnylam Pharmaceuticals (United States)
2023
New Generation University College
2001-2023
National University College
2006-2023
Massachusetts General Hospital
2022-2023
Abstract Single-cell transcriptome profiling of tumour tissue isolates allows the characterization heterogeneous cells along with neighbouring stromal and immune cells. Here we adopt this powerful approach to breast cancer analyse 515 from 11 patients. Inferred copy number variations single-cell RNA-seq data separate carcinoma non-cancer At a resolution, display common signatures within as well intratumoral heterogeneity regarding subtype crucial cancer-related pathways. Most are cells,...
Abstract Advanced metastatic cancer poses utmost clinical challenges and may present molecular cellular features distinct from an early-stage cancer. Herein, we single-cell transcriptome profiling of lung adenocarcinoma, the most prevalent histological type diagnosed at stage IV in over 40% all cases. From 208,506 cells populating normal tissues or early to 44 patients, identify a cell subtype deviating differentiation trajectory dominating stage. In stages, stromal immune dynamics reveal...
Abstract Microsatellite instability (MSI) refers to the hypermutability of short repetitive sequences in genome caused by impaired DNA mismatch repair. Although MSI has been studied for decades, large amounts sequencing data now available allows us examine molecular fingerprints greater detail. Here, we analyse ∼8,000 exomes and ∼1,000 whole genomes cancer patients across 23 types. Our analysis reveals that frequency events is highly variable within tumour We also identify genes repair...
A number of recent reports have demonstrated that only CD133-positive cancer cells glioblastoma multiforme (GBM) tumor-initiating potential. These findings raise an attractive hypothesis GBMs can be cured by eradicating stem (CSCs), which are a small portion GBM cells. However, as known to possess various genetic alterations, might harbor heterogeneous CSCs with different alterations. Here, we compared the clinical characteristics two patient groups divided according cell ratios. The...
The genome of an anonymous Korean male has been sequenced using a broad spread genomic techniques. This combinatorial approach allows for detailed characterization sequence and structural variation. first four individual genomes to have determined spanned three distinct ethnic groups: Yoruba African, northwest European (Craig Venter James Watson) Han Chinese. new work, together with another reported elsewhere, adds the Altaic grouping list. Human sequences so far individuals ancestry in...
Abstract Background Intra-tumoral genetic and functional heterogeneity correlates with cancer clinical prognoses. However, the mechanisms by which intra-tumoral impacts therapeutic outcome remain poorly understood. RNA sequencing (RNA-seq) of single tumor cells can provide comprehensive information about gene expression single-nucleotide variations in individual cells, may allow for translation heterogeneous cell responses into customized anti-cancer treatments. Results We isolated 34...
Abstract The consensus molecular subtype (CMS) classification of colorectal cancer is based on bulk transcriptomics. underlying epithelial cell diversity remains unclear. We analyzed 373,058 single-cell transcriptomes from 63 patients, focusing 49,155 cells. identified a pervasive genetic and transcriptomic dichotomy malignant cells, distinct gene expression, DNA copy number regulatory network. recapitulated these subtypes in 3,614 patients. two intrinsic subtypes, iCMS2 iCMS3, refine CMS....
Characterization of intratumoral heterogeneity is critical to cancer therapy, as the presence phenotypically diverse cell populations commonly fuels relapse and resistance treatment. Although genetic variation a well-studied source heterogeneity, functional impact most alterations remains unclear. Even less understood relative importance other factors influencing such epigenetic state or tumor microenvironment. To investigate relationship between transcriptional in context progression, we...
Frequent discrepancies between preclinical and clinical results of anticancer agents demand a reliable translational platform that can precisely recapitulate the biology human cancers. Another critical unmet need is ability to predict therapeutic responses for individual patients. Toward this goal, we have established library orthotopic glioblastoma (GBM) xenograft models using surgical samples GBM These patient-specific tumors histopathological properties maintain genomic characteristics...
Intratumoral heterogeneity hampers the success of marker-based anticancer treatment because targeted therapy may eliminate a specific subpopulation tumor cells while leaving others unharmed. Accordingly, rational strategy minimizing survival drug-resistant is essential to achieve long-term therapeutic efficacy. Using single-cell RNA sequencing (RNA-seq), we examine intratumoral pair primary renal cell carcinoma and its lung metastasis. Activation drug target pathways demonstrates...
Accurate detection of genomic alterations using high-throughput sequencing is an essential component precision cancer medicine. We characterize the variant allele fractions (VAFs) somatic single nucleotide variants and indels across 5095 clinical samples profiled a custom panel, CancerSCAN. Our results demonstrate that significant fraction clinically actionable have low VAFs, often due to tumor purity treatment-induced mutations. The percentages mutations under 5% VAF hotspots in EGFR, KRAS,...
Abstract Sequence alterations in microsatellites and an elevated mutational burden are observed 20% of gastric cancers associated with clinical response to anti–PD-1 antibodies. However, 50% microsatellite instability–high (MSI-H) intrinsically resistant PD-1 therapies. We conducted a phase II trial pembrolizumab patients advanced MSI-H cancer included serial multi-region tissue samples addition peripheral blood analyses. The number whole-exome sequencing (WES)–derived nonsynonymous...
Nonalcoholic fatty liver disease (NAFLD) is a major risk factor for cancer; therefore, its prevention an important clinical goal. Ablation of phosphatase and tensin homolog (PTEN) or the protein kinase Hippo signaling pathway induces cancer via activation AKT transcriptional regulators YAP/TAZ, respectively; however, potential crosstalk between PTEN/AKT Hippo/YAP/TAZ pathways in tumorigenesis has thus far remained unclear. Here, we have shown that deletion both PTEN SAV1 accelerates...
Abstract Breast cancer (BC) in the Asia Pacific regions is enriched younger patients and rapidly rising incidence yet its molecular bases remain poorly characterized. Here we analyze whole exomes transcriptomes of 187 primary tumors from a Korean BC cohort (SMC) pre-menopausal perform systematic comparison with primarily Caucasian post-menopausal (TCGA). SMC harbors higher proportions HER2+ Luminal B subtypes, lower proportion A decreased ESR1 expression compared to TCGA. We also observe...
Abstract Background Tumor cell-intrinsic mechanisms and complex interactions with the tumor microenvironment contribute to therapeutic failure via evolution. It may be possible overcome treatment resistance by developing a personalized approach against relapsing cancers based on comprehensive analysis of cell type-specific transcriptomic changes over clinical course disease using single-cell RNA sequencing (scRNA-seq). Methods Here, we used scRNA-seq depict landscape single case...
Abstract To elucidate the effects of neoadjuvant chemotherapy (NAC), we conduct whole transcriptome profiling coupled with histopathology analyses a longitudinal breast cancer cohort 146 patients including 110 pairs serial tumor biopsies collected before treatment, after first cycle treatment and at time surgery. Here, show that cytotoxic chemotherapies induce dynamic changes in immune microenvironment vary by subtype pathologic response. Just one induces an stimulatory harboring more...
Chemotherapy is ubiquitous in first-line treatment of advanced gastric cancer, yet responses are heterogeneous, and little known about mediators chemotherapy response. To move forward, an understanding the effects standard on tumor-immune microenvironment (TME) needed. Coupling whole-exome sequencing, bulk RNA single-cell transcriptomics from paired pretreatment on-treatment samples treatment-naïve patients with HER2-positive HER2-negative we define features associated response to...