A5037844323- Multiple Myeloma Research and Treatments
- Immune cells in cancer
- Chemokine receptors and signaling
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Macrophage Migration Inhibitory Factor
- GDF15 and Related Biomarkers
- Ferroptosis and cancer prognosis
- Medicinal Plant Pharmacodynamics Research
- Cancer Mechanisms and Therapy
- Quinazolinone synthesis and applications
- Muscle Physiology and Disorders
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Malaria Research and Control
- Wnt/β-catenin signaling in development and cancer
- Psoriasis: Treatment and Pathogenesis
- MicroRNA in disease regulation
- Health, Environment, Cognitive Aging
KU Leuven
2022-2025
Universitair Ziekenhuis Leuven
2023-2024
Samsung Medical Center
2017-2023
Samsung (South Korea)
2020-2023
Sungkyunkwan University
2019-2023
Sookmyung Women's University
2013-2017
Abstract Advanced metastatic cancer poses utmost clinical challenges and may present molecular cellular features distinct from an early-stage cancer. Herein, we single-cell transcriptome profiling of lung adenocarcinoma, the most prevalent histological type diagnosed at stage IV in over 40% all cases. From 208,506 cells populating normal tissues or early to 44 patients, identify a cell subtype deviating differentiation trajectory dominating stage. In stages, stromal immune dynamics reveal...
Abstract The consensus molecular subtype (CMS) classification of colorectal cancer is based on bulk transcriptomics. underlying epithelial cell diversity remains unclear. We analyzed 373,058 single-cell transcriptomes from 63 patients, focusing 49,155 cells. identified a pervasive genetic and transcriptomic dichotomy malignant cells, distinct gene expression, DNA copy number regulatory network. recapitulated these subtypes in 3,614 patients. two intrinsic subtypes, iCMS2 iCMS3, refine CMS....
Abstract Gastric cancer (GC) patients develop malignant ascites as the disease progresses owing to peritoneal metastasis. GC with have a rapidly deteriorating clinical course short survival following onset of ascites. Better optimized treatment strategies for this subset are needed. To define cellular characteristics GC, we used single-cell RNA sequencing characterize tumor cells and tumor-associated macrophages (TAMs) from four samples one sample cerebrospinal fluid. Reference...
Abstract CD8 + T cell activation via immune checkpoint blockade (ICB) is successful in microsatellite instable (MSI) colorectal cancer (CRC) patients. By comparison, the success of immunotherapy against stable (MSS) CRC limited. Little known about most critical features cells that together determine diverse landscapes and contrasting ICB responses. Hence, we pursued a deep single mapping on transcriptomic receptor (TCR) repertoire levels patient cohort, with additional surface proteome...
Somatic mutation is a major cause of cancer progression and varied responses tumors against anticancer agents. Thus, we must obtain characterize genome-wide mutational profiles in individual subtypes. The Cancer Genome Atlas database includes large amounts sequencing omics data generated from diverse human tissues. In the present study, integrated analyzed exome ~3,000 tissue samples summarized mutant genes each subtypes stages. Mutations were observed most (~23,000 genes) with low frequency...
Abstract Purpose: In multiple myeloma, extramedullary progression is associated with treatment resistance and a high mortality rate. To understand the molecular mechanisms controlling devastating of we applied single-cell RNA-sequencing (RNA-seq) to myeloma in bone marrow myelomatous pleural effusions or ascites. Experimental Design: Bone samples were collected from 15 patients subjected RNA-seq. The transcriptome data malignant plasma cells surrounding immune microenvironment analyzed....
Abstract Background Immune checkpoint inhibitors unleash inhibitory signals on T cells conferred by tumors and surrounding stromal cells. Despite the clinical efficacy of inhibitors, lack target expression persistence immunosuppressive limit pervasive effectiveness therapy. These limitations may be overcome alternative approaches that co-stimulate immune microenvironment. Methods We analyzed single-cell RNA sequencing data from multiple human cancers a mouse tumor transplant model to...
Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role subjecting to hydrolysis UPS. Recently, it has been demonstrated that specific class termed U-box are directly associated with mechanisms abiotic biotic stresses several plants. However, no studies on have performed one staple crops, barley. In this study, we identified 67 putative from barley...
Cancerstem-like cells (CSLCs) contribute to the initiation and recurrence of tumors their resistance conventional therapies. In this study, small interfering RNA (siRNA)-based screening ~4800 druggable genes in 3-dimensional CSLC cultures comparison 2-dimensional bulk U87 glioma revealed 3 groups essential for following: survival population only, bulk-cultured or both populations. While diverse biologic processes were associated with siRNAs reducing population, CSLC-eliminating enriched a...
234 Background: The majority of currently available biomarkers in colorectal cancer (CRC) perform poorly with respect to prognostication and prediction response. Immunoscore (IS) has been proposed as a signature host immune response reflecting favorable prognosis. Next, through AtezoTRIBE, ‘Immunoscore checkpoint’ (IS-IC) was introduced, indicating its potential predictive value for checkpoint blockade (ICB) pMMR metastatic CRC. IS-IC is computed quantitative spatial variables related...
<title>Abstract</title> In the colonic epithelium, fetal programming is induced by Yes-associated protein (YAP) signaling, which associated with tumorigenesis and progression in colorectal cancer (CRC). While CRC was long considered a WNT driven disease, here we show that programmed cells activated YAP signaling can arise independently of activation. Fetal cells, presence hyper- or hypo-activated WNT, have different characteristics are poor relapse. Furthermore, using various mouse models,...
Abstract The thyroid gland plays a critical role in the maintenance of whole-body metabolism. However, aging frequently impairs homeostatic by hormones due to increased prevalence subclinical hypothyroidism associated with mitochondrial dysfunction, inflammation, and fibrosis. To understand specific aging-related changes endocrine function epithelial cells, we performed single-cell RNA sequencing (RNA-seq) 54 726 cells derived from pathologically normal tissues 7 patients who underwent...
To elucidate the epigenetic mechanisms of drug resistance, epigenetically reprogrammed H460 cancer cells (R-H460) were established by transient introduction reprogramming factors. Then, R-H460 induced to differentiate withdrawal stem cell media for various durations, which resulted in differentiated (dR-H460). Notably, dR-H460 13 days (13dR-H460 cells) formed a significantly greater number colonies showing resistance both cisplatin and paclitaxel, whereas 40 (40dR-H460 lost resistance; this...
Hyeran Shim, Kiwon Jang, Yeong Hak Bang, Hoang Bao Khanh Chu, Jisun Kang, Jin-Young Lee, Sheehyun Cho, Hong Seok Jongbum Jeon, Taeyeon Hwang, Soobok Joe, Jinyeong Lim, Ji-Hye Choi, Eun Hye Joo, Kyunghee Park, Ji Hwan Moon, Kyung Yeon Han, Yourae Hong, Woo Yong Hee Cheol Kim, Seong Hyeon Yun, Beom Yoon Ah Jung Wook Huh, Kyong Shin, Dae Pyo, Hyekyung Hae-Ock Woong-Yang Jin Ok Yang & Young-Joon Kim. BMB Reports -0001;0:. https://doi.org/10.5483/BMBRep.2023-0093
In normal intestines, a fetal/regenerative/revival cell state can be induced upon inflammation. This plasticity in fate is also one of the current topics human colorectal cancer (CRC). To dissect underlying mechanisms, we generated CRC organoids with naturally selected genetic mutation profiles and exposed them to two different conditions by modulating extracellular matrix (ECM). Among tested profiles, was following YAP activation via collagen type I-enriched microenvironment....
e15587 Background: An analysis of colorectal cancer epithelial cells showed two intrinsic subtypes called iCMS2 and iCMS3, in addition to the bulk consensus (CMS1-4). The are prognostically important. We present a robust method for calculating iCMS individual samples, as opposed batches, applicable clinical setting. Methods: selected subset genes (N = 201) with strongest expression cancer, aiming reduce signal from other cell types. then calculated gene centroids by taking per-gene mean 8...
3592 Background: Second-line treatment with aflibercept and FOLFIRI in patients metastatic colorectal carcinoma (mCRC) previously treated oxaliplatin improved overall survival (OS) versus placebo [Hazard ratio (HR), 0.81; 95% CI, 0.71–0.93; P=0.003] 1 . However, cancer (CRC) is a heterogenous disease the new intrinsic consensus molecular (iCMS) allows for tumours to be subtyped into unique genetic transcriptional features 2 We present post hoc analysis of VELOUR patient data, assess impact...
Cell surface proteins have provided useful targets and biomarkers for advanced cancer therapies. The recent clinical success of antibody-drug conjugates (ADCs) highlights the importance finding selective antigens given subtypes. We thus attempted to develop stand-alone software analysis cell transcriptome patient samples prioritize lineage- and/or mutation-specific over-expression markers in cells. A total 519 genes were selected as proteins, their expression was profiled 14 subtypes using...
Summary In colorectal cancer (CRC), RNF43 mutations are linked to BRAF V600E-initiated serrated adenomas that advance into mucinous adenocarcinomas with poor prognosis upon metastasis. How facilitate a metastasis-prone growth state remains unknown. Here, we addressed this issue by repairing mutant in patient-derived BRAF-mutant CRC organoids using gene editing. Upon correction, exhibit strongly decreased mucus production and, moreover, display loss of niche factor independence and metastatic...