A5027494362- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Growth Hormone and Insulin-like Growth Factors
- Chronic Lymphocytic Leukemia Research
- Microtubule and mitosis dynamics
- Fibroblast Growth Factor Research
- Biochemical Analysis and Sensing Techniques
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Proteoglycans and glycosaminoglycans research
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Long-Term Effects of COVID-19
- Ferroptosis and cancer prognosis
- Gene Regulatory Network Analysis
- Genomics and Chromatin Dynamics
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- Protein Tyrosine Phosphatases
Zhejiang University
2022-2025
Women's Hospital, School of Medicine, Zhejiang University
2021-2025
Zhejiang Cancer Hospital
2023-2025
KU Leuven
2011-2023
Hangzhou Cancer Hospital
2023
VIB-KU Leuven Center for Cancer Biology
2019-2021
Catholic University of America
2019
Abstract How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild 26 critical in comparison BALs non-COVID-19 pneumonia normal lung. We use pseudotime inference build T-cell monocyte-to-macrophage trajectories model gene expression changes along them. In COVID-19, CD8 + resident-memory (T RM ) CD4...
Understanding the pathology of COVID-19 is a global research priority. Early evidence suggests that respiratory microbiome may be playing role in disease progression, yet current studies report contradictory results. Here, we examine potential confounders by analyzing upper (n = 58) and lower 35) tract well-phenotyped patients controls combining sequencing, viral load determination, immunoprofiling. We find time intensive care unit type oxygen support, as well associated treatments such...
The combination of atezolizumab plus bevacizumab (atezo/bev) has dramatically changed the treatment landscape advanced HCC (aHCC), achieving durable responses in some patients. Using single-cell transcriptomics, we characterize intra-tumoural and peripheral immune context patients with aHCC treated atezo/bev. Tumours from are enriched for PDL1+ CXCL10+ macrophages and, based on cell-cell interaction analysis, express high levels CXCL9/10/11 predicted to attract CXCR3+ CD8+ effector-memory T...
Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal malignancy because of its aggressive nature and the paucity effective treatment options. Almost all registered drugs have proven ineffective in addressing needs patients with PDAC. This is result poor understanding unique tumor-immune microenvironment (TME) To identify druggable regulators immunosuppressive TME, we performed kinome- membranome-focused CRISPR screening using orthotopic PDAC models. Our data showed that...
The first cell-penetrating peptide that activates protein phosphatase-1 (PP1) by disrupting a subset of PP1 complexes in living cells has been developed. Activated rapidly dephosphorylates its substrates, counteracting kinase activity inside cells. Activation can thus be novel approach to study function and counteract Ser/Thr under pathologically increased signaling. phosphorylation proteins is fundamental signal transduction processes Malfunction these contributes the development progress...
Abstract RepoMan is a scaffold for signalling by mitotic phosphatases at the chromosomes. During (pro)metaphase, RepoMan-associated protein PP1 and PP2A-B56 regulate chromosome targeting of Aurora-B kinase RepoMan, respectively. Here we show that this task division critically dependent on phosphorylation Cyclin-dependent 1 (Cdk1), which reduces binding but facilitates recruitment PP2A-B56. The inactivation Cdk1 in early anaphase reverses phosphatase switch, resulting accumulation PP1-RepoMan...
Hypoxia is pervasive in cancer and other diseases. Cells sense adapt to hypoxia by activating hypoxia-inducible transcription factors (HIFs), but it still an outstanding question why cell types differ their transcriptional response hypoxia.
Abstract Mounting evidence has demonstrated that long noncoding RNAs (lncRNAs) are dysregulated and implicated in the occurrence development of a wide range human malignancies. LINC00461, novel cancerârelated lncRNA, been reported to be highly expressed serve as oncogene glioma; however, its biological role breast cancer (BC) remains obscure. This study aimed explore LINC00461 BC elucidate potential molecular mechanisms involved. In current study, was found significantly upregulated both...
Abstract Tumor stroma plays a critical role in fostering tumor progression and metastasis. Cancer-associated fibroblasts (CAFs) are major component of the stroma. Identifying key molecular determinants for pro-tumor properties CAFs could enable development more effective treatment strategies. Herein, through analyses single-cell sequencing data, we identified population expressing high levels sulfatase 1 (SULF1), which was associated with poor prognosis colorectal cancer (CRC) patients. CRC...
Leber hereditary optic neuropathy (LHON) is a paradigm for mitochondrial retinopathy due to DNA (mtDNA) mutations. However, the mechanism underlying retinal cell-specific effects of LHON-linked mtDNA mutations remains poorly understood and there has been no effective treatment or cure this disorder. Using mice model bearing ND6P25L mutation, we demonstrated that mutation caused deficiencies, especially in ganglion cells (RGC), rods MĂźller cells. Single-cell RNA sequencing revealed...
Batch effect correction (BEC) is fundamental to integrate multiple single-cell RNA sequencing datasets, and its success critical empower in-depth interrogation for biological insights. However, no simple metric available evaluate BEC performance with sensitivity data overcorrection, which erases true variations leads false discoveries. Here, we propose RBET, a reference-informed statistical framework evaluating the of BEC. Using extensive simulations six real examples including scRNA-seq...