A5100620996- Immunotherapy and Immune Responses
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Ion Channels and Receptors
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Anesthesia and Neurotoxicity Research
- Catalytic Processes in Materials Science
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Yersinia bacterium, plague, ectoparasites research
- CO2 Reduction Techniques and Catalysts
- CAR-T cell therapy research
- Ginseng Biological Effects and Applications
- RNA Interference and Gene Delivery
- Plant Disease Resistance and Genetics
- Advanced oxidation water treatment
- Plant responses to elevated CO2
- Plant Pathogenic Bacteria Studies
- Bacterial biofilms and quorum sensing
- Plant responses to water stress
- Environmental remediation with nanomaterials
- Ginger and Zingiberaceae research
- Genetically Modified Organisms Research
State Forestry and Grassland Administration
2025
Sichuan Agricultural University
2025
Peking University
2025
Peking University Third Hospital
2025
Yueyang Hospital
2020-2024
Hainan Medical University
2024
Hubei Academy of Agricultural Sciences
2023-2024
Baoding People's Hospital
2024
Shanghai University of Traditional Chinese Medicine
2020-2024
Jiangsu Academy of Agricultural Sciences
2023
Plants deploy receptor-like kinases and nucleotide-binding leucine-rich repeat receptors to confer host plant resistance (HPR) herbivores1. These gene-for-gene interactions between insects their hosts have been proposed for more than 50 years2. However, the molecular cellular mechanisms that underlie HPR elusive, as identity sensing of insect avirulence effectors remained unknown. Here we identify an salivary protein perceived by a immune receptor. The BPH14-interacting (BISP) from brown...
The type III secretion system (T3SS) is a clinically important virulence mechanism in Pseudomonas aeruginosa that secretes and translocates up to four protein toxin effectors into human cells, facilitating the establishment dissemination of infections. To discover inhibitors this mechanism, we developed two cellular reporter assays applied them library 80,000 compounds. primary screen was based on dependence transcription T3SS operons T3SS-mediated negative regulator consisted...
New vaccine approaches are needed for Pseudomonas aeruginosa, which continues to be a major cause of serious pulmonary infections. Although Th17 cells can protect against gram-negative pathogens at mucosal surfaces, including the lung, bacterial proteins recognized by largely unknown and could potential new candidates.We describe strategy identify Th17-stimulating protein antigens aeruginosa assess their efficacy as vaccines pneumonia.Using library in vitro transcribed translated P....
TNF antagonists may offer therapeutic potential in solid tumors, but patients who have high serum levels of TNF-α fail to respond infliximab, suggesting consumption the circulating antibody and loss transmembrane (tmTNF-α) on tumors by ectodomain shedding. Addressing this possibility, we developed a monoclonal (mAb) that binds both full-length tmTNF-α its N-terminal truncated fragment membrane after processing does not cross-react with soluble TNF-α. We documented expression primary breast...
Abstract RepoMan is a scaffold for signalling by mitotic phosphatases at the chromosomes. During (pro)metaphase, RepoMan-associated protein PP1 and PP2A-B56 regulate chromosome targeting of Aurora-B kinase RepoMan, respectively. Here we show that this task division critically dependent on phosphorylation Cyclin-dependent 1 (Cdk1), which reduces binding but facilitates recruitment PP2A-B56. The inactivation Cdk1 in early anaphase reverses phosphatase switch, resulting accumulation PP1-RepoMan...
ABSTRACT Pseudomonas aeruginosa is responsible for potentially life-threatening infections in individuals with compromised defense mechanisms and those cystic fibrosis. P. infection notable the appearance of a humoral response to some known antigens, such as flagellin C, elastase, alkaline protease, others. Although number immunogenic proteins are known, no effective vaccine has been approved yet. Here, we report comprehensive study all 262 outer membrane exported PAO1 by modified protein...
ABSTRACT A number of bacterial pathogens utilize the type III secretion pathway to deliver effector proteins directly into host cell cytoplasm. Certain strains Pseudomonas aeruginosa associated with acute infections express a potent cytotoxin, exoenzyme U (ExoU), that is delivered via contacting cells. Once inside mammalian cell, ExoU rapidly lyses intoxicated cells its phospholipase 2 (PLA ) activity. high-throughput cell-based assay was developed screen libraries compounds for those...
Transient receptor potential vanilloid‐1 (TRPV1) was first identified in sensory neurons, where it suggested as a therapeutic target for pain relief. Here, we show that TRPV1 is expressed the pancreatic cancer cell line, PANC‐1; epidermal growth factor (EGFR) expression downregulated by overexpression or agonist‐induced activation of TRPV1; and conversely, EGFR increased after silencing TRPV1. Furthermore, inhibits proliferation significantly reduces mRNA levels two oncogenes, KRAS AKT2 ....
The incidence of early childhood caries shows a significant increasing trend. Often, children younger than 6 years need additional help to finish the dental treatment. Therefore, general anesthesia (GA) could provide successful environment for pediatric treatment.The aim this study was assess effect treatment under (DGA) on patients' oral health in Chongqing, Southwest China using P-CPQ and FIS questionnaires.We collected hospital's records who received DGA from 2013 2014 questionnaires...
Abstract Objectives Ginsenoside Rg1 (GRg1), one of the major active constituents Panax notoginseng, has shown anti-inflammatory and antinocioceptic activity, but its role in keratinocytes needs further study. We have examined inhibitory effect GRg1 on transient receptor potential vanilloid-1 (TRPV1) activation keratinocyte HaCaT cells explored involved mechanism. Methods HEK 293T over-expressing exogenous TRPV1 were constructed named 293T-TRPV1 cells. The effects production interleukin-8...
A chitosan-based Cu2+ fluorescent probe was designed and synthesized independently using the C-2-amino group of chitosan with 1, 8-naphthalimide derivatives. series experiments were conducted to characterize optical properties grafted probe. The fluorescence quenching effect investigated based on interactions between common metals. It found that proposed displayed selective interaction over other metal ions anions, reaching equilibrium within 5 min.
Using two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis, we found that copper/zinc superoxide dismutase (Cu/Zn-SOD, SOD-1) was induced in constructed CCR5 stably transfected HEK 293 cells, but not mock treated with CCL5. CCL5-induced SOD-1 expression also confirmed 293-CCR5 cells and CCR5-positive granulocyte-macrophage colony-stimulating factor-induced human macrophages murine macrophage RAW264.7 cells. CCL5 interaction mainly via MEK-ERK activation. In addition,...
Blowflies found on human corpses are important for the estimation of postmortem interval and other questions forensic relevance. Some these species difficult to differentiate morphologically, therefore a molecular method was elaborated identification. Here, we describe rapid identification insects. Specific insect DNA fragments were amplified using polymerase chain reaction, followed by direct sequencing amplification products. Analysis cytochrome oxidase II sequences revealed abundant...
Mulberry is a valuable woody plant with significant economic importance. It can be propagated through two main methods: cutting and grafting. Waterlogging have major impact on mulberry growth significantly reduce production. In this study, we examined gene expression patterns photosynthetic responses in three waterlogged cultivars Compared to the control group, waterlogging treatments reduced levels of chlorophyll, soluble protein, sugars, proline, malondialdehyde (MDA). Additionally,...
Mulberry leaves are excellent for health care, confirmed as a ‘drug homologous food’ by the Ministry of Health, China. The bitter taste mulberry is one main problems that hinders development food industry. bitter, unique difficult to eliminate post-processing. In this study, metabolites in were identified flavonoids, phenolic acids, alkaloids, coumarins and L-amino acids combined analysis metabolome transcriptome leaves. differential showed diverse sugar down-regulated, indicating was...
Background: Butyric acid (BT), a short-chain fatty acid, is the preferred colonocyte energy source. The effects of BT on differentiation, proliferation, and apoptosis small intestinal epithelial cells piglets its underlying mechanisms have not been fully elucidated. Methods: In this study, it was found that 0.2-0.4 mM promoted differentiation procine jejunal (IPEC-J2) cells. at 0.5 or higher concentrations significantly impaired cell viability in dose- time-dependent manner. addition, high...
Horizontal gene transfer (HGT) has been well documented as a driving force in the evolution of bacteria. It shown that horizontally acquired gene, xoc_2868, involved global response against oxidative stress and pathogenicity Xanthomonas oryzae pv. oryzicola strain BLS256. However, transcriptional factor (TF), regulatory mechanism XOC_2868 not yet revealed. Here, evolutionary analysis suggested might be co-transferred with its physically proximate downstream genes from Burkholderiaceae...
The present study aimed to determine the taxonomic positions of strains designated R-5-52-3T, R-5-33-5-1-2, R-5-48-2 and R-5-51-4 isolated from hot spring water samples. Cells these were Gram-stain-negative, non-motile rod-shaped. shared highest 16S rRNA gene sequence similarity with Vulcaniibacterium thermophilum KCTC 32020T (95.1%). Growth occurred at 28-55 °C, pH 6-8 up 3 % (w/v) NaCl. DNA fingerprinting, biochemical, phylogenetic analyses suggested that different but belonged same...