Yonglun Luo
A5036195699- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Renal and related cancers
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Pancreatic and Hepatic Oncology Research
- Genetic factors in colorectal cancer
- Bladder and Urothelial Cancer Treatments
- Molecular Biology Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
Aarhus University
2016-2025
Aarhus University Hospital
2019-2025
Steno Diabetes Centers
2019-2025
Chinese Academy of Sciences
2012-2025
BGI Group (China)
2018-2024
University of Electronic Science and Technology of China
2014-2024
BGI Research
2023-2024
University of Chinese Academy of Sciences
2009-2024
Shenzhen Pingle Orthopedic Hospital
2024
University of Copenhagen
2024
Single-cell RNA analysis has been integrated with spatial protein profiling to create a single–cell type map of human tissues.
Mapping the mammalian brain The diverse physiology of is reflected in its complex organization at regional, cellular, and subcellular levels. Sjöstedt et al. combined data—both newly acquired from other large-scale mapping projects—from transcriptomics, single-cell genomics, situ hybridization, antibody-based protein profiling to map molecular profiles human, pig, mouse brain. analysis consistent with a conserved basic architecture during evolution, but it does show differences regional gene...
Taking the PERVs out of pigs With severe shortage organs needed for transplants, xenotransplantation (transplantation nonhuman to humans) offers an alternative source. Some pig have similar size and function those humans. The challenge is that genome harbors porcine endogenous retroviruses (PERVs) can potentially pass humans with possibly damaging consequences. Niu et al. generated in which all copies were inactivated by CRISPR-Cas9 engineering (see Perspective Denner). Not only does this...
Abstract Antiviral strategies to inhibit Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2) and the pathogenic consequences of COVID-19 are urgently required. Here, we demonstrate that NRF2 antioxidant gene expression pathway is suppressed in biopsies obtained from patients. Further, uncover agonists 4-octyl-itaconate (4-OI) clinically approved dimethyl fumarate (DMF) induce a cellular antiviral program potently inhibits replication SARS-CoV2 across cell lines. The inhibitory effect...
The transcription factor Nrf2 is a critical regulator of inflammatory responses. If and how also affects cytosolic nucleic acid sensing currently unknown. Here we identify as an important negative STING suggest link between metabolic reprogramming antiviral DNA in human cells. Here, activation decreases expression responsiveness to agonists while increasing susceptibility infection with viruses. Mechanistically, regulates by decreasing mRNA stability. Repression occurs metabolically...
Significance Statement The specialized vessels comprising the renal vasculature are characterized by highly differentiated endothelial cell types, but this heterogeneity has been poorly inventoried. Using single-cell RNA sequencing, authors developed a high-resolution atlas of mouse cells. They also investigated how medullary cells adapt to switch from diuresis antidiuresis. This study describes molecular and metabolic adaptation dehydration, uncovers role for mitochondrial oxidative...
Abstract The design of CRISPR gRNAs requires accurate on-target efficiency predictions, which demand high-quality gRNA activity data and efficient modeling. To advance, we here report on the generation for 10,592 SpCas9 gRNAs. Integrating these with complementary published data, train a deep learning model, CRISPRon, 23,902 Compared to existing tools, CRISPRon exhibits significantly higher prediction performances four test datasets not overlapping training used development tools....
Abstract The Farm Animal Genotype-Tissue Expression (FarmGTEx) project has been established to develop a public resource of genetic regulatory variants in livestock, which is essential for linking polymorphisms variation phenotypes, helping fundamental biological discovery and exploitation animal breeding human biomedicine. Here we show results from the pilot phase PigGTEx by processing 5,457 RNA-sequencing 1,602 whole-genome sequencing samples passing quality control pigs. We build pig...
Abstract Since a detailed inventory of endothelial cell (EC) heterogeneity in breast cancer (BC) is lacking, here we perform single RNA-sequencing 26,515 cells (including 8433 ECs) from 9 BC patients and compare them to published EC taxonomies lung tumors. Angiogenic ECs are phenotypically similar, while other subtypes different. Predictive interactome analysis reveals known but also previously unreported receptor-ligand interactions between immune cells, suggesting an involvement responses....
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent (hiPS-MSCs). The hiPS-MSCs expressed markers (CD90, CD73 and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic chondrogenic lost pluripotency – as seen the loss of OCT3/4 TRA-1-81 tumorigenicity. However, these iPS-MSCs are still positive marker NANOG. further explored osteogenic potential in...
Abstract Introduction Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective this study is to systematically characterize the characteristics by comparing them bone marrow-derived MSCs (BM-MSCs). Methods Two iPSC-MSC lines (named as mRNA-iPSC-MSC-YL001 lenti-iPSC-MSC-A001) one BM-MSC line used study. Cell...
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with disorders. However, there is a lack of engineering tools to recapitulate study the biogenesis eccDNAs. Here, we created dual-fluorescence biosensor cassette, which upon delivery pairs CRISPR/Cas9 guide RNAs, CRISPR-C, allows us specific fluorophore expressing eccDNA human cells. We show that CRISPR-C can generate functional eccDNA, using novel system. further reveal also eccDNAs from...
Abstract Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has been used for epigenome editing, but specificities these dCas9 methyltransferases have not fully investigated. Findings We generated CRISPR-guided by fusing catalytic domain DNMT3A or DNMT3B C terminus from Streptococcus pyogenes and validated its on-target global off-target characteristics. Using targeted...