A5014329497- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Landslides and related hazards
- Advanced Photocatalysis Techniques
- Vestibular and auditory disorders
- Electrocatalysts for Energy Conversion
- Bladder and Urothelial Cancer Treatments
- RNA regulation and disease
- Colorectal Cancer Treatments and Studies
- Hearing, Cochlea, Tinnitus, Genetics
- Soil and Unsaturated Flow
- Immune Response and Inflammation
- Geophysical and Geoelectrical Methods
- Earthquake Detection and Analysis
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Nanomaterials for catalytic reactions
Chinese University of Hong Kong
2022-2025
South China University of Technology
2025
University of Copenhagen
2022-2025
Zhejiang University
2023-2025
The Seventh Affiliated Hospital of Sun Yat-sen University
2023-2025
Sun Yat-sen University
2023-2025
Beijing Luhe Hospital Affiliated to Capital Medical University
2025
Westlake University
2024-2025
Xinjiang University
2023-2025
Nanjing University
2025
Abstract The design of CRISPR gRNAs requires accurate on-target efficiency predictions, which demand high-quality gRNA activity data and efficient modeling. To advance, we here report on the generation for 10,592 SpCas9 gRNAs. Integrating these with complementary published data, train a deep learning model, CRISPRon, 23,902 Compared to existing tools, CRISPRon exhibits significantly higher prediction performances four test datasets not overlapping training used development tools....
The electrochemical CO2 reduction reaction (CO2RR) offers a green and sustainable process to convert into valuable chemical stocks fuels. Metal is one of the most promising types catalysts drive an efficient selective CO2RR. catalytic performance metal nanocatalysts strongly dependent on their structural features. Recently, phase engineering nanomaterials (PEN) has emerged as prominent tactic regulate for A broad range with conventional unconventional crystal phases been developed,...
Abstract Hypertriglyceridaemia is a very rare disorder caused by the mutations of LPL gene, with an autosomal recessive mode inheritance. Here, we identified two unrelated Chinese patients manifested severe hypertriglyceridaemia and acute pancreatitis. The clinical symptoms proband 1 are more than 2. Whole exome sequencing Sanger were performed. Functional analysis has been done. pairs variants in gene (c.162C>A c.1322+1G>A) 2 (c.835C>G c.1322+1G>A). substitution (c.162C>A)...
Extrachromosomal DNA (ecDNA) presents a promising target for cancer therapy; however, its spatial-temporal diversity and influence on tumor evolution the immune microenvironment remain largely unclear. We apply computational methods to analyze ecDNA from whole-genome sequencing data of 595 urothelial carcinoma (UC) patients. demonstrate that drives clonal through structural rearrangements during malignant transformation recurrence UC. This supports model wherein tumors evolve via selective...
Abstract Meckel syndrome (MKS) is a pre‐ or perinatal multisystemic ciliopathic lethal disorder with an autosomal recessive mode of inheritance. usually manifested meningo‐occipital encephalocele, polycystic kidney dysplasia, postaxial polydactyly and hepatobiliary ductal plate malformation. Germline variants in CEP290 cause MKS4. In this study, we investigated 35‐years‐old Chinese female who was 17+1 weeks pregnant. She had history adverse pregnancy having foetus multiple malformations. We...
Extrachromosomal circular DNAs (eccDNAs) carrying random genomic segments are broadly found across different cancer types, but their molecular functions and impact in gastric (GC) rarely known. In this study, we aimed to investigate the potential role of eccDNA GC. Using Circle-seq strategy, observed abundance tissues (GCT) was aberrantly higher than that normal adjacent (NAT). The high eccDNAs oncogene-segments GCT may represent DNA damage products amplified oncogenes. Analysis...
Objective: To investigate the relationship between hearing loss and vestibular dysfunction in patients with sudden sensorineural (SSHL).Methods: Clinical data including symptom of vertigo 149 SSHL were investigated retrospectively. Pure tone audiometry, ocular vestibular-evoked myogenic potential (oVEMP) cervical (cVEMP) evoked by air-conducted sound (ACS), caloric test employed for cochlear function assessment. The level was analyzed.Results: pure averages (PTAs) (mean ± SD) without 88.81...
Cancer is a disease of genome instability and genomic alterations; now, heterogeneity rapidly emerging as defining feature cancer, both within between tumors. Motivation for our pilot study tumor in esophageal squamous cell carcinoma (ESCC) that it not well studied, but the highest incidences cancers are found China ESCC most common type. We profiled mutations changes copy number were identified by whole-exome sequencing array-based comparative hybridization multiple regions an from two...
Abstract Background There is a need for functional genome-wide annotation of the protein-coding genes to get deeper understanding mammalian biology. Here, new strategy introduced based on dimensionality reduction and density-based clustering whole-body co-expression patterns. This has been used explore gene expression landscape in pig, we present map all major pig tissues organs. Results An open-access ( www.rnaatlas.org ) presented 350 samples across 98 well-defined divided into 44 tissue...
The prevalence of Lynch syndrome (LS) varies significantly in different populations, suggesting that ethnic features might play an important role. We enrolled 3330 consecutive Chinese patients who had surgical resection for newly diagnosed colorectal cancer. Universal screening LS was implemented, including immunohistochemistry mismatch repair (MMR) proteins, BRAFV600E mutation test and germline sequencing. Among the 3250 eligible patients, MMR protein deficiency (dMMR) detected 330 (10.2%)...
Methods for sensitive and high-throughput evaluation of CRISPR RNA-guided nucleases (RGNs) off-targets (OTs) are essential advancing RGN-based gene therapies. Here we report SURRO-seq simultaneously evaluating thousands therapeutic RGN OTs in cells. captures RGN-induced indels cells by pooled lentiviral libraries deep sequencing, an approach comparable complementary to detection T7 endonuclease 1, GUIDE-seq, CIRCLE-seq. Application 8150 from 110 RGNs identifies significantly detectable 783...
Abstract Tumor residue and tissue damage normally occurred after surgical treatment of malignant melanoma, the effective postoperative therapy is still a challenge because requests simultaneous but opposite manipulation tumor cells healthy cells. Herein, MBGP‐Gel, thermosensitive biodegradable hydrogel incorporating S‐nitrosoglutathione (GSNO) loaded N‐aminoethyl‐N’‐benzoyl thiourea (BTU) modified MSNs (MBGP NPs), was designed to utilize significant difference copper content between regulate...
Objective: Extrachromosomal circular DNA elements (eccDNAs) are known for their broad existence in cells and plasma, which may potentially play important roles many biological processes. Our aim was to identify functional or marked eccDNAs gout patients. Methods: The Circle-Seq approach applied eccDNA detection from plasma acute patients healthy controls. Further analysis performed on the distribution of genomic gene annotations two groups. Results: We detected 57,216 109,683 control...
Abstract Urine‐based liquid biopsy has emerged as a non‐invasive and effective tool for early screening diagnosis of bladder cancer. This review provides comprehensive overview the current urine‐based biomarkers methods detection monitoring We focus on including tumour DNAs, proteins, microbiome, RNAs, long non‐coding transfer RNA‐derived fragments, messenger microRNAs, circular exosomes extrachromosomal DNA.
Gastric cancer (GC) is the third leading cause of death worldwide. Its clinical course varies considerably due to highly heterogeneous tumour microenvironment (TME). Decomposing complex TME from histological images into its constituent parts crucial for evaluating patterns and enhancing GC therapies. Although various deep learning methods were developed in medical field, their applications on this task are hindered by lack well-annotated GC. Through work, we seek provide a large database...