A5069773948- Muscle Physiology and Disorders
- 3D Printing in Biomedical Research
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Diabetes Treatment and Management
- Pancreatic function and diabetes
- Cleft Lip and Palate Research
- Drug Transport and Resistance Mechanisms
- Craniofacial Disorders and Treatments
- Diet and metabolism studies
- Pharmacological Effects and Toxicity Studies
- Erythropoietin and Anemia Treatment
- Total Knee Arthroplasty Outcomes
- Acute Ischemic Stroke Management
- Sirtuins and Resveratrol in Medicine
- Osteoarthritis Treatment and Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- MRI in cancer diagnosis
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
Aarhus University
2016-2024
Aarhus University Hospital
2014-2024
Steno Diabetes Centers
1996-2023
Orthopaedic Research
2014
Metformin is the most widely prescribed oral antiglycemic drug, with few adverse effects. However, surprisingly little known about its human biodistribution and target tissue metabolism. In animal experiments, we have shown that metformin can be labeled by 11C 11C-metformin PET used to measure renal function. Here, extend these preclinical findings a first-in-human dosimetry, biodistribution, kinetics study.Nine subjects (3 women 6 men) participated in 2 studies: first study, radiation...
Metformin is the most commonly prescribed oral antidiabetic drug, with well-documented beneficial preventive effects on diabetic complications. Despite being in clinical use for almost 60 years, underlying mechanisms metformin action remain elusive. Organic cation transporters (OCT), including multidrug and toxin extrusion proteins (MATE), are essential transport of across membranes, but tissue-specific activity these vivo incompletely understood. Here, we dynamic positron emission...
Abstract A porcine calvaria defect study was carried out to investigate the bone repair potential of three‐dimensional (3D)‐printed poly‐ε‐caprolactone (PCL) scaffolds embedded with nanoporous PCL. microscopic grid network created by rapid prototyping making a 3D‐fused deposition model (FDM‐PCL). Afterward, FDM‐PCL were infused mixture PCL, water, and 1,4‐dioxane underwent thermal‐induced phase separation (TIPS) followed lyophilization. The TIPS process lead structure shielded printed...
Background Numerous treatment methods for osteochondral repair have been implemented, including auto- and allogeneic transplantations, combined bone chondrocyte synthetic implants, but no gold standard has established. We present preliminary data on a autologous cartilage chips: dual-tissue transplantation (ADTT); an easily applicable, low-cost option repair. The aim of this study was to investigate the early biological clinical outcome ADTT. Materials Eight patients (age 32 ± 7.5 years)...
NAD+ is a co-factor and substrate for enzymes maintaining energy homeostasis. Nicotinamide phosphoribosyltransferase (NAMPT) controls synthesis, in skeletal muscle, essential muscle integrity. However, the underlying molecular mechanisms by which synthesis affects health remain poorly understood. Thus, objective of current study was to delineate role NAMPT-mediated biosynthesis development function. To determine Nampt function, we generated muscle-specific KO (SMNKO) mice. We performed...
In this study, we sought to assess the osteogenic potential of human dental pulp stem cells (DPSCs) on three different polycaprolactone (PCL) scaffolds. The backbone structure scaffolds was manufactured by fused deposition modeling (PCL scaffold). composition and morphology functionalized in two first underwent thermal induced phase separation PCL infused into pores scaffold. This procedure resulted a highly variable micro- nanostructured porous (NSP), interconnected, isotropic tubular...
Aims To test the hypothesis that brown adipose tissue (BAT) is a metformin target by investigating in vivo uptake of [ 11 C]‐metformin tracer mice and studying vitro effects on cultured human adipocytes. Materials methods Tissue‐specific was assessed PET/CT imaging after injection C]‐metformin. Human obtained from elective neck surgery transporter expression levels murine BAT were determined qPCR. Oxygen consumption metformin‐treated adipocyte cell models Seahorse XF technology. Results...
The osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs) was compared with that dental pulp-derived (DPSCs) in vitro and a pig calvaria critical-size defect model.BMSCs DPSCs were extracted from the tibia marrow molar teeth each pig, respectively. BMSCs cultured monolayer on three-dimensional (3D) polycaprolactone (PCL) - hyaluronic acid tricalcium phosphate (HT-PCL) scaffold. Population doubling (PD), alkaline phosphatase (ALP) activity, calcium deposition...
BACKGROUNDDuring aging, there is a functional decline in the pool of muscle stem cells (MuSCs) that influences and regenerative capacity skeletal muscle. Preclinical evidence has suggested nicotinamide riboside (NR) pterostilbene (PT) can improve regeneration, e.g., by increasing MuSC function. The objective this study was to investigate if supplementation with NR PT (NRPT) promotes regeneration after injury elderly individuals improved recruitment MuSCs.METHODSThirty-two (55-80 years age)...
We have reviewed at an average period of ten years the results 71 consecutive primary arthroplasties with Insall-Burstein total condylar knee prosthesis in patients rheumatoid arthritis. Their mean age surgery was 52 (24 to 72). At follow-up overall (Hospital for Special Surgery rating score) were excellent or good 77%, fair 11% and poor 11%. There residual pain only 5% prostheses situ; 58% could walk more than 500 m, median range motion 108 degrees. Eight knees had been revised. Five...
Abstract The type-2 diabetes drug metformin has proven to have protective effects in several renal disease models. Here, we investigated the a 3-day unilateral ureteral obstruction (3dUUO) mouse model. Compared with controls, obstructed animals displayed increased tubular damage and inflammation. Metformin treatment attenuated inflammation, anti-oxidative response decreased damage. Hepatic uptake depends on expression of organic cation transporters (OCTs). To test whether kidney are...
Background and purpose — The osteogenic potency of erythropoietin (EPO) has been documented. However, its efficacy in a large-animal model not yet investigated; nor clinically safe dosage. this study was to overcome such limitations previous studies thereby pave the way for possible clinical application. Our hypothesis that EPO increases calvarial bone healing compared saline control same subject.Methods We used porcine defect model. In each 18 pigs, 6 cylindrical defects (diameter: 1 cm;...
Diffusion kurtosis imaging (DKI) is sensitive to tissue microstructure and may therefore be useful in the diagnosis monitoring of disease brain body organs. Generally, diffusion magnetic resonance (dMRI) challenging because heterogeneous composition, which can cause image artefacts as a result chemical shifts susceptibility differences. In addition, abdomen possesses physiological factors (e.g. breathing, heartbeat, blood flow) severely reduce quality, especially when echo planar employed,...
Abstract We tested the hypothesis that erythropoietin (EPO) enhances bone formation after posterolateral spinal fusion (PLF) in a rabbit model. Thirty‐four adult rabbits underwent intertransverse arthrodesis at L5–L6 level using 2.0 g autograft per side. The animals were randomly divided into two groups receiving subcutaneous daily injections of either EPO or saline for 20 days. Treatment commenced 2 days preoperatively. Hemoglobin was monitored baseline and 2, 4, 6 weeks surgery. After...
The anti-diabetic biguanide drugs metformin (METF) and phenformin (PHEN) may have anti-cancer effects. Biguanides suppress plasma growth factors, but nonetheless, the view that these mitochondrial inhibitors accumulate in tumor tissue to an extent leads severe energetic stress or alleviation of hypoxia-induced radioresistance is gaining ground. Our cell studies confirm biguanides inhibits proliferation by targeting respiration, only at highly suprapharmacological concentrations due low drug...
To evaluate the clinical and biological outcome of combined bone marrow aspirate concentrate (BMAC) platelet-rich plasma (PRP) on a collagen scaffold for treating cartilage lesions in knee.Ten patients (mean age 29.4 years, range 18-36) suffering from large full-thickness knee were treated with BMAC PRP January 2015 to December 2016. In 1-step procedure autologous was seeded onto sutured into debrided defect. Patients evaluated by scores (IKDC [International Knee Documentation Committee...
Organic cation transporters (OCTs) in the kidney proximal tubule (PT) participate renal excretion of drugs and endogenous compounds. PT function is commonly impaired diseases, consequently quantitative measurement OCT may provide an important estimate function. Metformin a widely used drug targets type 2 located PT. Thus, we hypothesized that <sup>11</sup>C-labeled metformin would be suitable PET tracer for quantification <b>Methods:</b><sup>11</sup>C-metformin was prepared by...
Animal models clearly illustrate that the maintenance of skeletal muscle mass depends on function and interaction a heterogeneous population resident infiltrating mononuclear cells. Several lines evidence suggest cells also play role in wasting humans, targeting these may open new treatment options for intervention or prevention sarcopenia. Methodological ethical constraints have perturbed exploration cellular characteristics human muscle. Thus, investigations phenotypes often depend...