A5084280756- Single-cell and spatial transcriptomics
- Adipokines, Inflammation, and Metabolic Diseases
- Crystallization and Solubility Studies
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- X-ray Diffraction in Crystallography
- Synthetic Organic Chemistry Methods
- Atherosclerosis and Cardiovascular Diseases
- Nanoparticle-Based Drug Delivery
- Immune Response and Inflammation
- Chemical synthesis and alkaloids
- Adipose Tissue and Metabolism
- Crystallography and molecular interactions
- Advanced Synthetic Organic Chemistry
- Ferroptosis and cancer prognosis
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular Health and Disease Prevention
- Blood Pressure and Hypertension Studies
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Computational Drug Discovery Methods
- S100 Proteins and Annexins
- Analytical Methods in Pharmaceuticals
Science for Life Laboratory
2018-2025
KTH Royal Institute of Technology
2015-2025
UiT The Arctic University of Norway
2019-2023
Karolinska Institutet
2022-2023
Karolinska University Hospital
2022-2023
University College London
2015
King's College London
2009
Institute of Molecular Biology and Biophysics
2009
Guy's Hospital
2009
Transnational Press London
2009
Single-cell RNA analysis has been integrated with spatial protein profiling to create a single–cell type map of human tissues.
The importance of defining cell-type-specific genes is well acknowledged. Technological advances facilitate high-resolution sequencing single cells, but practical challenges remain. Adipose tissue composed primarily adipocytes, large buoyant cells requiring extensive, artefact-generating processing for separation and analysis. Thus, adipocyte data are frequently absent from single-cell RNA (scRNA-seq) datasets, despite being the primary functional cell type. Here, we decipher...
Abstract Venous thromboembolism (VTE) is a common, multi-causal disease with potentially serious short- and long-term complications. In clinical practice, there need for improved plasma biomarker-based tools VTE diagnosis risk prediction. Here we show, using proteomics profiling to screen from patients suspected acute VTE, several case-control studies how Complement Factor H Related 5 protein (CFHR5), regulator of the alternative pathway complement activation, VTE-associated biomarker....
Changes in the endothelium of cerebral vasculature can contribute to inflammatory, thrombotic, and malignant disorders. The importance defining cell-type-specific genes their modification disease is increasingly recognized. Here, we develop a bioinformatics-based approach identify normal brain cell-enriched genes, using bulk RNA sequencing (RNA-seq) data from 238 human cortex samples 2 independent cohorts. We compare endothelial gene profiles with astrocyte, oligodendrocyte, neuron,...
The total syntheses of (+/-)-aspercyclide A (1) and its C19 methyl ether (15a) featuring Heck-Mizoroki macrocyclisation to form the 11-membered (E)-styrenyl biaryl lactone core are described.
Abstract The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis reveal that constitutively, highly-selectively, adult human endothelial cells (EC), independent proliferative status. Correspondingly, demonstrate it not marker for tumour EC multiple malignancy types. Imaging from different vascular beds reveals...
SUMMARY Genes with cell type specific expression typically encode for proteins that have functions. Single RNAseq (scRNAseq) has facilitated the identification of such genes, but various challenges limit analysis certain types and lowly expressed genes. Here, we performed an integrative network over 6000 bulk datasets from 15 human organs, to generate a tissue-by-tissue enrichment prediction atlas all protein coding We profile major constituent types, including several are fragile or...
Inflammation is an important risk-associated component of many diseases and can be diagnosed by molecular imaging specific molecules. The aim this study was to evaluate the possibility targeting adhesion molecules on inflammation-activated endothelial cells macrophages using innovative multimodal polyvinyl alcohol-based microbubble (MB) contrast agent developed for diagnostic use in ultrasound, magnetic resonance, nuclear imaging. We assessed binding efficiency antibody-conjugated inflamed...
Background: Inflammation and accumulation of macrophages are key features unstable atherosclerotic plaques. The ability to take up molecular probes can be exploited in new clinical imaging methods for the detection lesions. We investigated whether modifications human serum albumin (HSA) could used target efficiently vitro. Materials methods: Maleylated aconitylated HSA were compared with unmodified HSA. Fluorescent or radiolabeled (89Zr) modified was vitro experiments study cellular uptake...
Endothelial cell (EC) dysfunction is a well-established response to cardiovascular disease risk factors, such as smoking and obesity. Risk factor exposure can modify EC signaling behavior, leading arterial venous development. Here, we aimed identify biomarker panels for the assessment of dysfunction, which could be useful stratification or monitor treatment response. Approach Results: We used affinity proteomics proteins circulating in plasma that were associated with exposure. Two hundred...
The identification of cell type-specific genes and their modification under different conditions is central to our understanding human health disease. stomach, a hollow organ in the upper gastrointestinal tract, provides an acidic environment that contributes microbial defence facilitates activity secreted digestive enzymes process food nutrients into chyme. In contrast other sections detailed descriptions type gene enrichment profiles stomach are absent from major single-cell...
The vascular endothelium acts as a dynamic interface between blood and tissue. TNF-α, major regulator of inflammation, induces endothelial cell (EC) transcriptional changes, the overall response dynamics which have not been fully elucidated. In present study, we conducted an extended time-course analysis human EC to TNF, from 30 min 72 h. We identified regulated genes used weighted gene network correlation decipher coexpression profiles, uncovering two distinct temporal phases: acute...
Vascular injury is a serious complication of sepsis due to the gram-negative bacterium Neisseria meningitidis. One critical early steps in initiating this via interaction leucocytes, particularly neutrophils, with adhesion molecules expressed on inflamed endothelium. We have previously demonstrated that both lipopolysaccharide (LPS) and non-LPS components meningococci can induce very high levels expression vascular endothelial cell molecule E-selectin, which for tethering capture neutrophils...
SUMMARY The identification of cell type-specific genes and their modification under different conditions is central to our understanding human health disease. stomach, a hollow organ in the upper gastrointestinal tract, provides an acidic environment that contributes microbial defence facilitates activity secreted digestive enzymes process food nutrients into chyme. In contrast other sections detailed descriptions type gene enrichment profiles stomach are absent from major single...
ABSTRACT The vascular endothelium acts as a dynamic interface between blood and tissue provides an anti-inflammatory anti-thrombotic surface under normal conditions. Tumour necrosis factor-α (TNF), cytokine that drives acute chronic inflammation, induces numerous transcriptional changes in endothelial cells (EC). However, the overall temporal dynamics of this response have not been fully elucidated. Here, we conducted extended time-course analysis EC to TNF, from 30 minutes 72 hours. We...
Aspercyclide A is one of three fungal metabolites to be isolated from Aspergillus sp. found in soil extracts 2004 and has been shown have micromolar potency as a small molecule antagonist for blocking protein-protein interaction that triggers an inflammatory mediator release. (+)-Aspercyclide C previously synthesized using ring-closing metathesis effect macrocyclization (A. Fürstner, C. Müller Chem. Commun. 2005, 5583). Recently, (+)-asper-cyclides B also synthesized using...
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ABSTRACT Changes in the endothelium of cerebral vasculature can contribute to inflammatory, thrombotic and malignant disorders. The importance defining cell type-specific genes how they are modified disease is increasingly recognised. Here, we developed a bioinformatics-based approach identify normal brain cell-enriched genes, using bulk RNAseq data from 238 human cortex samples 2 independent cohorts. We compared endothelial gene profiles with astrocyte, oligodendrocyte neuron profiles....
Changes in the endothelium of cerebral vasculature can contribute to inflammatory, thrombotic and malignant disorders. The importance defining cell type-specific genes how they are modified disease is increasingly recognised. Here, we developed a bioinformatics-based approach identify normal brain cell-enriched genes, using bulk RNAseq data from 238 human cortex samples 2 independent cohorts. We compared endothelial gene profiles with astrocyte, oligodendrocyte neuron profiles. Global...