A5035853200- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Antifungal resistance and susceptibility
- Bacterial biofilms and quorum sensing
- Transgenic Plants and Applications
- Fungal Infections and Studies
- Bone Tissue Engineering Materials
- Protein Hydrolysis and Bioactive Peptides
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Microfluidic and Bio-sensing Technologies
- Antimicrobial agents and applications
- Sphingolipid Metabolism and Signaling
- Dental Implant Techniques and Outcomes
- Orthopedic Infections and Treatments
- Oral microbiology and periodontitis research
- Electrowetting and Microfluidic Technologies
- Dental materials and restorations
- Insect and Pesticide Research
- Microfluidic and Capillary Electrophoresis Applications
- Diet, Metabolism, and Disease
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Plant Pathogens and Fungal Diseases
- Probiotics and Fermented Foods
KU Leuven
2016-2025
Ecologie Microbienne Lyon
2020
Institute of Plant and Microbial Biology, Academia Sinica
2012
University of Bonn
2011
Janssen (Belgium)
1995-2007
University of Kentucky
2000
Abstract Peptides with antimicrobial properties are present in most if not all plant species. All peptides isolated so far contain even numbers of cysteines (4, 6, or 8), which pairwise connected by disulfide bridges, thus providing high stability to the peptides. Based on homologies at primary structure level, can be classified into distinct families including thionins, defensins, lipid transfer proteins, and he vein- knottin-type Detailed three-dimensional information has been obtained for...
From seeds of Aesculus hippocastanum, Clitoria ternatea, Dahlia merckii and Heuchera sanguinea five antifungal proteins were isolated shown to be homologous plant defensins previously characterised from radish γ‐thionins Poaceae seeds. Based on the spectrum their antimicriobial activity morphological distortions they induce fungi peptides can divided into two classes. The did not inhibit any three different α‐amylases.
Abstract How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild 26 critical in comparison BALs non-COVID-19 pneumonia normal lung. We use pseudotime inference build T-cell monocyte-to-macrophage trajectories model gene expression changes along them. In COVID-19, CD8 + resident-memory (T RM ) CD4...
Epidemiological and clinical reports indicate that SARS-CoV-2 virulence hinges upon the triggering of an aberrant host immune response, more so than on direct virus-induced cellular damage. To elucidate immunopathology underlying COVID-19 severity, we perform cytokine multiplex profiling in patients. We show hypercytokinemia differs from interferon-gamma-driven storm macrophage activation syndrome, is pronounced critical versus mild-moderate COVID-19. Systems modelling levels paired with...
ABSTRACT We used an assay based on the uptake of SYTOX Green, organic compound that fluoresces upon interaction with nucleic acids and penetrates cells compromised plasma membranes, to investigate membrane permeabilization in fungi. Membrane induced by plant defensins Neurospora crassa was biphasic, depending defensin dose. At high levels (10 40 μM), strong detected could be strongly suppressed cations medium. This appears rely direct peptide-phospholipid interactions. lower (0.1 1 a weaker,...
An antimicrobial protein of about 10 kD, called Ace-AMP1, was isolated from onion (Allium cepa L.) seeds. Based on the near-complete amino acid sequence this protein, oligonucleotides were designed for polymerase chain reaction-based cloning corresponding cDNA. The mature is homologous to plant nonspecific lipid transfer proteins (nsLTPs), but it shares only 76% residues that are conserved among all known nsLTPs and unusually rich in arginine. Ace-AMP1 inhibits 12 tested pathogenic fungi at...
Growth of the yeast species Candida albicans and Pichia pastoris is inhibited by RsAFP2, a plant defensin isolated from radish seed (Raphanus sativus), at micromolar concentrations. In contrast, gcs-deletion mutants both are resistant toward RsAFP2. GCS genes encode UDP-glucose:ceramide glucosyltransferases, which catalyze final step in biosynthesis membrane lipid glucosylceramide. an enzyme-linked immunosorbent assay-based binding assay, RsAFP2 was found to interact with glucosylceramides...
Treatment of hyphae Neurospora crassa with antifungal plant defensins, i.e. Rs-AFP2 and Dm-AMP1 isolated from radish dahlia seed, respectively, induced a rapid K+ efflux, Ca2+ uptake, alkalinization the incubation medium. The Rs-AFP2-induced medium could be inhibited G-protein inhibitors. α-Hordothionin, an thionin barley caused sustained increased uptake at subinhibitory concentrations but only transient inhibitory concentrations. α-Hordothionin also efflux medium, these fluxes occurred...
Although thionins and 2S albumins are generally considered as storage proteins, both classes of seed proteins known to inhibit the growth pathogenic fungi. We have now found that wheat (Triticum aestivum L.) or barley (Hordeum vulgare thionin concentration required for 50% inhibition fungal is lowered 2- 73-fold when combined with (at sub- noninhibitory concentrations) from radish (Raphanus sativus oilseed rape (Brassica napus L.). Furthermore, antifungal activity synergistically enhanced...
RsAFP2 (<i>Raphanus sativus </i>antifungal peptide 2), an antifungal plant defensin isolated from seed of <i>R. sativus</i>, interacts with glucosylceramides (GlcCer) in membranes susceptible yeast and fungi induces membrane permeabilization fungal cell death. However, using carboxyfluorescein-containing small unilamellar vesicles containing purified GlcCer, we could not observe as a consequence insertion such vesicles. Therefore, focused on putative RsAFP2-induced...
Summary The antifungal plant defensin RsAFP2 isolated from radish interacts with fungal glucosylceramides and induces apoptosis in Candida albicans . To further unravel the mechanism of action tolerance mechanisms, we screened a library 2868 heterozygous C. deletion mutants identified 30 RsAFP2‐hypersensitive mutants. most prominent group genes was involved cell wall integrity hyphal growth/septin ring formation. Consistent these genetic data, demonstrated that , which also contains...
We investigated the cellular mechanisms responsible for occurrence of miconazole-tolerant persisters in Candida albicans biofilms. Miconazole induced about 30% killing sessile C. cells at 75 μM. The fraction persisters, i.e., that can survive high doses miconazole (0.6 to 2.4 mM), these biofilms was 1 2%. Since induces reactive oxygen species (ROS) cells, we focused on a role superoxide dismutases (Sods) persistence and found expression Sod-encoding genes by compared levels untreated cells....