A5072651011- Arsenic contamination and mitigation
- Skin and Cellular Biology Research
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Effects and risks of endocrine disrupting chemicals
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Animal testing and alternatives
- Pluripotent Stem Cells Research
- Lung Cancer Treatments and Mutations
- Molecular Biology Techniques and Applications
- Mesenchymal stem cell research
- Heavy Metal Exposure and Toxicity
- Biological Stains and Phytochemicals
- Hair Growth and Disorders
- Nanoparticles: synthesis and applications
- Head and Neck Cancer Studies
- Genetic factors in colorectal cancer
- T-cell and B-cell Immunology
- Retinoids in leukemia and cellular processes
- Dermatological and Skeletal Disorders
- Nonmelanoma Skin Cancer Studies
- Carcinogens and Genotoxicity Assessment
- Historical Gender and Feminism Studies
- Cell Image Analysis Techniques
Thomas Jefferson University
2019-2024
Sidney Kimmel Cancer Center
2021
National Cancer Center
2015-2020
Core Laboratories (United States)
2018
Indian Institute of Toxicology Research
2010-2015
Integral University
2014
Faculty of 1000 (United States)
2010
Circulating tumor DNA (ctDNA) has emerged as a candidate biomarker for cancer screening. However, studies on the usefulness of ctDNA postoperative recurrence monitoring are limited. The present study monitored in blood by employing cancer-specific rearrangements. Personalized rearrangements 25 gastric cancers were analyzed whole-genome sequencing (WGS) and employed with up to 12 months after surgery. identified 19 samples. median lead time, which is duration between positive detection...
Lack of type VII collagen (C7) disrupts cellular proteostasis yet the mechanism remains undescribed. By studying relationship between C7 and extracellular matrix (ECM)-associated proteins thrombospondin-1 (TSP1), XII (C12) tissue transglutaminase (TGM2) in primary human dermal fibroblasts from multiple donors with or without genetic disease recessive dystrophic epidermolysis bullosa (RDEB) (n=31), we demonstrate that secretion each these is increased presence C7. In isolated patients RDEB,...
Squamous cell carcinomas of the head and neck (HNSCC) arise from mucosal keratinocytes upper aero-digestive tract. Despite a common origin similar driver-gene mutations which divert fate differentiation to proliferation, HNSCC are considered heterogeneous group tumors categorized by site within mucosa, presence or absence HPV infection. Tobacco use is major driver carcinogenesis in poor prognosticator that has previously been associated with immune infiltration higher mutation numbers. Here,...
Of late, a consirable interest has grown in literature on early development of arsenicosis and untimely death humans after exposure to iAs drinking water utero or during the childhood. The mechanism this kind intrauterine arsenic poisoning is not known; however it often suggested involve stem cells. We looked into possibility by investigating mice influence chronic arsenical preliminarily multipotent adult cell progenitor counts at beginning neonatal age. found that repeated intake 42.5 85...
Recently, we reported cytoskeleton-associated protein2 (CKAP2) as a possible new prognostic breast cancer marker. However, it has not yet been applied in clinic. Therefore, clinical significance of CKAP2 was evaluated comparison with that Ki-67 cohort patients, and the expression difference analyzed cell cycle-arrested fibroblast cells.A total 579 early patients who underwent surgery at National Cancer Center Hospital Korea between 2001 2005 were accrued. CKAP2-positive count (CPCC) labeling...
The importance of hypomethylation in SLE is well recognized; however, the significance hypermethylation has not been characterized. We screened hypermethylated marks and investigated their possible implications.DNA methylation were whole-blood DNA by microarray, two ( CD3Z VHL hypermethylations) confirmed a single-base extension method independent ethnic cohorts consisting 207 patients 151 controls. correlation with clinical manifestations genetic influence on those epigenetic analysed.Two...
To elucidate the epigenetic mechanisms of drug resistance, epigenetically reprogrammed H460 cancer cells (R-H460) were established by transient introduction reprogramming factors. Then, R-H460 induced to differentiate withdrawal stem cell media for various durations, which resulted in differentiated (dR-H460). Notably, dR-H460 13 days (13dR-H460 cells) formed a significantly greater number colonies showing resistance both cisplatin and paclitaxel, whereas 40 (40dR-H460 lost resistance; this...
Proliferation activity has already been established as a prognostic marker or for anticancer drug sensitivity. In gastric cancer, however, the significance of proliferation is still being debated. Several studies evaluating using Ki-67 have shown controversial results in terms relationship between and overall survival (OS) sensitivity cancer patients. Because cytoskeleton-associated protein 2 (CKAP2) staining recently introduced activity, we analyzed 437 tissues through CKAP2...
Immunofluorescent staining is currently the method of choice for determination protein expression levels in cell-culture systems when morphological information also necessary. The protocol immunocytochemical on paraffin-embedded cell blocks, presented herein, an excellent alternative to immunofluorescent non-paraffin-embedded fixed cells. In this protocol, a paraffin block from HeLa cells was prepared using thromboplastin-plasma method, and immunocytochemistry performed evaluation two...
We examined the clastogenic and cell-transforming potential of ultrafine particulate matter fraction PM0.056 urban ambient aerosol using mammalian cells. PM1.0, PM0.56 fractions were sampled from roadside atmosphere an area cascade impactor MOUDI-NR-110. The to induce cytotoxicity, DNA damage micronuclei formation was at test concentrations 3, 6, 12.5, 25, 50 100 μg/ml 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide...
A method to characterize and culture stem cells from neonate mouse epidermis after transplacental BrdU (bromo-deoxyuridine) administration is described. We have characterized by their properties viz. retain label, adhere rapidly onto collagen-fibronectin substratum express a specific biomarker beta-1-integrin. BrdU-labelled (detected using monoclonal antibody) constituted sum of 18% the total number cells. The ability freshly isolated keratinocytes [LRCs (label-retaining cells)] bind primary...
Abstract A method to characterize and culture stem cells from neonate mouse epidermis after transplacental BrdU (bromo-deoxyuridine) administration is described. We have characterized by their properties viz. retain label, adhere rapidly onto collagen—fibronectin substratum express a specific biomarker beta-1-integrin. BrdU-labelled (detected using monoclonal antibody) constituted sum of 18% the total number cells. The ability freshly isolated keratinocytes [LRCs (label-retaining cells)]...
We have shown both LLC1 expression in the lung epithelium by situ hybridization and its inactivation cancer epigenetic modification. However, protein's cellular localization or role normal tissues has not yet been evaluated. In present study, a monoclonal antibody against recombinant was produced, immunohistochemical staining performed on arrays including various human tissues, non-small cell (NSCLC) for localization. The results showed cilia of normal-airway epithelial cells cytoplasm type...
Immunofluorescent staining is currently the method of choice for determination protein expression levels in cell-culture systems when morphological information also necessary. The protocol immunocytochemical on paraffin-embedded cell blocks, presented herein, an excellent alternative to immunofluorescent non-paraffin-embedded fixed cells. In this protocol, a paraffin block from HeLa cells was prepared using thromboplastin-plasma method, and immunocytochemistry performed evaluation two...
Arsenic is the most toxic metalloid present in natural environment both organic and inorganic arsenic forms. Inorganic often more hazardous than form. Arsenite arsenate compounds are major forms which causing severe human health dysfunction including cancer. Excretion of from system found elusive. Therefore, it interest to screen channel proteins with complex different combination arsenic, GSH (glutathione) selenium using docking methods. The mode removal. structure revealed binding...
A pure-population of keratinocyte stem cell culture is required for applications such as transgenic-mousestem preparation, therapy, tissue engineering, iPS technology, besides study growth & differentiation, toxicology, and related mechanistic aspects.Protocols available in literature do not conform to desired purity better yield short span time, culture-condition rapid growth, characterization methods . We rport here the improved, albeit comprehensive replicable protocols identify, isolate,...