A5102984562- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Simulation Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- dental development and anomalies
- Reproductive Biology and Fertility
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Renal and related cancers
- Silk-based biomaterials and applications
- Electrospun Nanofibers in Biomedical Applications
- Bone and Dental Protein Studies
- Bone Tissue Engineering Materials
- Xenotransplantation and immune response
- Astro and Planetary Science
- Cancer-related gene regulation
- Planetary Science and Exploration
- Epigenetics and DNA Methylation
- Oral and Maxillofacial Pathology
- Systems Engineering Methodologies and Applications
- Spacecraft Design and Technology
- Telomeres, Telomerase, and Senescence
- Sperm and Testicular Function
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
Seoul National University
2013-2025
University of Central Florida
2014-2024
Seoul National University Dental Hospital
2003-2024
Applied Materials (United States)
2024
Jet Propulsion Laboratory
2008-2020
Laboratory of Molecular Genetics
2014
Ono Pharmaceutical (United States)
2013
Envia Systems (United States)
2009
California Institute of Technology
2008
Xenobe Research Institute
2006
The nasolabial fold is absent in the face of newborn, disappears paralyzed face, but retained upon death. There very little information literature on what makes up fold. Four fresh cadavers were studied by taking a strip facial tissue from each at right angles to fold; all soft layers through included. Microscopic studies strips showed be made (1) dense fibrous tissue, (2) muscle fibers branching levator muscles upper lip, and (3) striated bundles originating fascia. also revealed lip...
MicroRNAs are novel key regulators of cellular differentiation. Dlx transcription factors play an important role in osteoblast differentiation, and Dlx5 Dlx2 known targets miR-124. Therefore, the present study, we investigated regulatory effects miR-124 on osteogenic differentiation vivo bone formation mesenchymal stem cells (MSCs). During induction by BMP2, expression levels were inversely correlated with those marker genes human mouse marrow-derived MSCs, MC3T3-E1 C2C12 cells. The...
To study the effects of decreased amounts or absence aldosterone on development and endocrine function, we have disrupted mouse gene, Cyp11b2, coding for synthase (AS) by replacing its first two exons with sequences enhanced green fluorescent protein. The null pups fail to thrive postnatally, about 30% die between d 7 28. Aldosterone in plasma AS mRNA adrenal glands are undetectable mice. Adult AS-null mice small, weigh 75% wild type, hypotensive, increased concentrations K(+)...
Natural IgM antibodies play an important role in the body's defense mechanisms against transformed cells human body and are currently being exploited both prognoses of malignant lesions therapy cancer patients. However, despite growing interest clinical promise, thus far class has failed to gain widespread commercial as these considered be difficult produce recombinantly. IgMs polymeric have a relatively large mass. In addition, molecules heavily glycosylated and, when produced non-human...
ObjectiveTo suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation.DesignProspective approach using animal model.SettingStem cell and bioevaluation laboratory, Seoul National University.Animal(s)F1 (C57BL6 X DBA2) outbred (ICR) mice.Intervention(s)Ovarian stroma of less than 40 μm in diameter were subcultured with fibroblast monolayer, colony-forming characterized.Main Outcome Measure(s)Stemness, genotype, imprinted gene...
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, mechanisms by which nanofibrous matrix promotes mesenchymal stem cell (MSC) are largely unknown. Here, we investigated underlie matrix-induced odontoblastic human dental pulp MSCs (DP-MSCs). An electrospun polystyrene (PSF) was prepared, responses PSF were assessed...
To explore the effects of decreased amounts or absence aldosterone, we have disrupted gene coding for aldosterone synthase (AS) in mice and investigated blood pressure kidney function AS +/+ , +/− −/− mice. normal pressures show no abnormalities electrolytes expression, but they significantly higher than urine volume lower osmolality. In contrast, low pressure, abnormal electrolyte homeostasis (increased plasma concentrations K + Ca 2+ Mg HCO 3 − Cl difference Na level), disturbances water...
Cystic fibrosis is an autosomal recessive disorder affecting chloride transport in pancreas, lung, and other tissues, which caused by mutations the cystic transmembrane conductance regulator (CFTR). Certain alkyl xanthines such as CPX (8-cyclopentyl-1,3-dipropylxanthine) stimulate Cl- efflux from cells bearing ΔF508 genotype common to most cases of fibrosis. We have hypothesized that CFTR molecule itself might be site for action, perhaps region first nucleotide binding fold (NBF-1) domain....
The periodontal ligament-derived mesenchymal stem cell is regarded as a source of adult cells due to its multipotency. However, the proof chondrogenic potential scarce. Therefore, we investigated differentiation capacity ligament derived induced by transforming growth factor (TGF)-β3 and bone morphogenetic protein (BMP)-6. After isolation (PDLSCs) from human ligament, were cultured in Dulbecco's modified Eagle's medium (DMEM) with 20% fetal bovine serum (FBS). A mechanical force initiated...
This study examined the influences of titanium (Ti) discs with similar surface roughnesses (R(a) values), but different topographies and chemical compositions, on adhesion, spreading, alkaline phosphatase (ALP) activity osteoblast-like cells normal human fibroblasts. The presence adhesion molecules Ti surfaces their effects cell were also investigated. Two types prepared. One kind was a mechanically polished disc, other type disc obtained by heating hydroxyapatite (HA) dip-coated Ti....
Abstract The objective of this study was to assess the practical usefulness morphologically poor oocytes (MPCOCs) in relation follicular size and oocyte diameter. Oocytes collected from medium (3–8 mm diameter) small (<3 mm) follicles were classified into five categories good (MGCOCs) (MA, control), MPCOCs with larger smaller diameters (ML MS, respectively), those (SL SS, respectively). examined for maturation developmental competence after parthenogenesis somatic cell nuclear transfer...
Tooth development and regeneration occur through reciprocal interactions between epithelial ectodermal mesenchymal stem cells. However, the current studies on tooth are limited, since cells relatively difficult to obtain maintain. Human embryonic (hESCs) induced pluripotent (hiPSCs) may be alternative options for cell sources. To differentiate hESCs/hiPSCs into dental epithelial-like cells, this study investigated hypothesis that direct such as hESCs or hiPSCs, Hertwig’s root...
Caveolin-1, a tumor suppressor gene, was found to be inactivated by structural abnormalities or epigenetic changes in several types of human cancers. In order investigate the role Caveolin-1 pathogenesis oral squamous cell carcinoma, exon 1 and 3 sequences were investigated 74 carcinomas 15 cancer lines. addition, expression examined Mutations gene identified five cancers (1 missense 4 silent mutations), with all mutations being detected 3. Among containing mutation, case 53 harbored...
Matrix extracellular phosphoglycoprotein (MEPE) is mainly expressed in mineralizing tissues, and its C-terminal proteolytic cleavage product an acidic-serine-asparate-rich-MEPE-associated motif (ASARM) that a strong regulator of body phosphate metabolism mineralization. There sufficient data supporting role for MEPE protein function mineralization, however, little known about the regulation <i>MEPE</i> gene expression. As bone morphogenetic protein-2 (BMP-2) one most important signals...
Abstract M atrix e xtracellular p hosphoglycoprot in (MEPE) is a specific marker of mineralizing osteoblasts and osteocytes. Canonical BMP Wnt signaling pathways are two the strongest paracrine signals stimulating osteogenesis. Our previous results indicated that Mepe expression stimulated by BMP‐2‐signaling pathway. The aim this study addressed whether also controlled signaling, there cross‐regulation between major osteogenic pathways. Treatment with Wnt3a, canonical stimulator, strongly...