A5006589634- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Bone health and treatments
- Bone and Dental Protein Studies
- dental development and anomalies
- Craniofacial Disorders and Treatments
- Bone Tissue Engineering Materials
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Mesenchymal stem cell research
- Dental Implant Techniques and Outcomes
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- Periodontal Regeneration and Treatments
- Cleft Lip and Palate Research
- S100 Proteins and Annexins
- Histone Deacetylase Inhibitors Research
- Alkaline Phosphatase Research Studies
- Heterotopic Ossification and Related Conditions
- Electrospun Nanofibers in Biomedical Applications
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- MicroRNA in disease regulation
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
Seoul National University
2016-2025
Seoul National University Dental Hospital
2011-2025
Laboratory of Molecular Genetics
2014-2018
Yonsei University
2012
National Research Institute of Police Science
2011
Kyungpook National University
1997-2009
Korea Basic Science Institute
2009
Pohang University of Science and Technology
2009
Scripps Research Institute
2009
Daegu Catholic University Medical Center
2008
When C2C12 pluripotent mesenchymal precursor cells are treated with transforming growth factor β1 (TGF-β1), terminal differentiation into myotubes is blocked. Treatment bone morphogenetic protein 2 (BMP-2) not only blocks myogenic of but also induces osteoblast differentiation. The molecular mechanisms governing the ability TGF-β1 and BMP-2 to both induce ligand-specific responses inhibit known. We identified Runx2/PEBP2αA/Cbfa1, a global regulator osteogenesis, as major TGF-β1-responsive...
Runx2/Cbfa1/Pebp2aA is a global regulator of osteogenesis and crucial for regulating the expression bone-specific genes. Runx2 major target bone morphogenetic protein (BMP) pathway. Genetic analysis has revealed that degraded through Smurf-mediated ubiquitination pathway, its activity inhibited by HDAC4. Here, we demonstrate molecular link between Smurf, HDACs Runx2, in BMP signaling. BMP-2 signaling stimulates p300-mediated acetylation, increasing transactivation inhibiting Smurf1-mediated...
Two homeotic genes, Dlx and Msx, appear to regulate development of mineralized tissues, including bone, cartilage, tooth. Expression Msx-1 Msx-2 has been studied during the osteoblast phenotype, but role in this context regulation bone-expressed genes is unknown. We used targeted differential display isolate family that are expressed at defined stages differentiation. These studies were carried out with fetal rat calvarial cells produce bone-like tissue vitro. observed a mineralization...
The bone morphogenetic protein (BMP)-2 is a potent osteoinductive signal, inducing formation in vivo and osteoblast differentiation from non-osseous cells vitro. runt domain-related Cbfa1/PEBP2αA/AML-3 critical component of transcriptional regulator differentiation. To investigate the relationship between extracellular BMP-2 Cbfa1, osteogenesis, we examined expression Cbfa1 osteoblastic genes during induced osteogenic transdifferentiation myoblastic cell line C2C12. treatment completely...
Fibroblast growth factor (FGF)/FGF receptor (FGFR) signaling induces the expression of Runx2, a key transcription in osteoblast differentiation, but little is known about molecular mechanisms that mediate this. Here we examined role protein kinase C (PKC) pathway regulating Runx2 gene and its transactivation function. Treatment with FGF2 or FGF4, transfection vector expressing mutant FGFR2 constitutively activated absence ligand, strongly stimulates expression. Electrophoretic mobility shift...
Bone formation involves several tightly regulated gene expression patterns of bone-related proteins. To determine the proteins during MC3T3-E1 osteoblast-like cell differentiation, we used Northern blotting, enzymatic assay, and histochemistry. We found that were in a temporal manner successive developmental stages including proliferation (days 4-10), bone matrix formation/maturation 10-16), mineralization 16-30). During period cell-cycle related genes such as histone H3 H4, ribosomal...
Alkaline phosphatase (ALP) is a widely accepted bone marker. Its expression stimulated by morphogenetic protein (BMP)-2 treatment, the activation of BMP receptors and R-Smads, Dlx5 Runx2. However, how BMP-2 induces ALP not clearly understood. We dissected murine promoter found within it Dlx5-binding cis-acting element electrophoretic mobility shift assays site-directed mutagenesis element. product its target gene, Runx2, activity in an additive manner. because continued to stimulate Runx2–/–...
Bone formation involves several tightly regulated gene expression patterns of bone-related proteins. To determine the proteins during MC3T3-E1 osteoblast-like cell differentiation, we used Northern blotting, enzymatic assay, and histochemistry. We found that were in a temporal manner successive developmental stages including proliferation (days 4–10), bone matrix formation/maturation 10–16), mineralization 16 –30). During period cell-cycle related genes such as histone H3 H4, ribosomal...
Two major isoforms of the Runx2 gene are expressed by alternative promoter usage: type I (Runx2-I) is derived from proximal (P2), and II (Runx2-II) produced distal (P1). Our previous results indicate that Dlx5 mediates BMP-2-induced expression osteoblast differentiation (Lee, M.-H., Kim, Y-J., H-J., Park, H-D., Kang, A-R., Kyung, H.-M., Sung, J-H., Wozney, J. M., Ryoo, H-M. (2003) Biol. Chem. 278, 34387-34394). However, little known molecular mechanisms which up-regulates in BMP-2 signaling....
Abstract Glycoproteins in human serum play fundamental roles many biological processes, and also have clinical value as biomarkers for disease progression treatment. In this study, we isolated glycoproteins from the sera of three healthy individuals lung adenocarcinoma patients using multilectin affinity chromatography. The recovered were subjected to treatment with peptide‐ N ‐glycosidase F (PNGase F) in‐gel digestion by trypsin. Tryptic peptides analyzed nano‐LC coupled ESI‐MS/MS MS/MS...
Runx2 is a critical transcription factor for osteoblast differentiation. Regulation of expression levels and transcriptional activity important bone morphogenetic protein (BMP)-induced Previous studies have shown that extracellular signal-regulated kinase (Erk) activation enhances the BMP-induced acetylation increases stability activity. Because BMP signaling induces Erk in osteoblasts, we sought to investigate whether regulates stability. by overexpression constitutively active MEK1...
Abstract Sclerostin decreases bone mass by antagonizing the Wnt signaling pathway. We examined whether obesity‐induced loss is associated with expression of sclerostin. Five‐week‐old male mice were assigned to one two groups (n = 10 each) and fed either a control diet (10% kcal from fat; CON) or high‐fat (60% HF) for 12 weeks. Thex final body weight whole fat HF higher than those CON mice. The distal femur cancellous mineral density formation rate was lower in percent erosion surface serum...
Cellular senescence is closely related to tissue aging including bone. Bone homeostasis maintained by the tight balance between bone-forming osteoblasts and bone-resorbing osteoclasts, but it undergoes deregulation with age, causing age-associated osteoporosis, a main cause of which osteoblast dysfunction. Oxidative stress caused accumulation reactive oxygen species (ROS) in bone tissues can accelerate However, regulatory mechanism that controls ROS-induced poorly understood. Here, we...
Although the normal physiological level of oxidative stress is beneficial for maintaining bone homeostasis, imbalance between reactive oxygen species (ROS) production and antioxidant defense can cause various diseases. The purpose this study was to determine whether nicotinamide (NAM), an NAD
Runx2 is a key transcription factor regulating osteoblast differentiation and skeletal morphogenesis, FGF2 one of the most important regulators development. The importance ERK mitogen-activated protein (MAP) kinase pathway in cranial suture development was demonstrated by findings that inhibition FGF/FGF receptor (FGFR) signaling MEK blocker prevents premature closure caused an Fgfr2 mutation mice. We previously activation does not affect <i>Runx2</i> gene expression but it stimulates...