A5101602565- melanin and skin pigmentation
- Skin and Cellular Biology Research
- Dermatology and Skin Diseases
- Skin Protection and Aging
- Hair Growth and Disorders
- Nail Diseases and Treatments
- Dermatologic Treatments and Research
- Cancer and Skin Lesions
- Cutaneous lymphoproliferative disorders research
- Wnt/β-catenin signaling in development and cancer
- Wound Healing and Treatments
- Genetic and rare skin diseases.
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Psoriasis: Treatment and Pathogenesis
- Cell Adhesion Molecules Research
- Acne and Rosacea Treatments and Effects
- Dermatological and Skeletal Disorders
- Blood properties and coagulation
- NF-κB Signaling Pathways
- Nonmelanoma Skin Cancer Studies
- Autoimmune Bullous Skin Diseases
- Cellular Mechanics and Interactions
- Biochemical Analysis and Sensing Techniques
- RNA regulation and disease
Chungnam National University Hospital
2000-2021
Chungnam National University
2011-2020
Daejeon University
2006
National Institutes of Health
1994-1996
Recently, autologous platelet-rich plasma (PRP) has attracted attention in various medical fields, including plastic and orthopedic surgery dermatology, for its ability to promote wound healing. PRP been tested during facelift hair transplantation reduce swelling pain increase density.To investigate the effects of on growth using vivo vitro models.PRP was prepared double-spin method applied dermal papilla (DP) cells. The proliferative effect activated DP cells measured. To understand...
Summary Although many hypo‐pigmenting agents are currently available, the demand for novel whitening is increasing, in part due to weak effectiveness and unwanted side effects of available compounds. To screen agents, methodologies such as cell culture enzymatic assays routinely used. However, these models have disadvantages terms physiological economic relevance. In this study, we validated zebrafish a whole‐animal model phenotype‐based screening melanogenic inhibitors or stimulators. We...
The transglutaminase (TGase) family of enzymes, which seven different members are known in the human genome, participate many biological processes involving cross-linking proteins into large macromolecular assemblies. TGase 2 enzyme is to be present neuronal tissues and may play a role degenerative diseases such as Alzheimer's disease (AD) by aberrantly proteins. In this paper, we demonstrate reverse transcriptase-polymerase chain reaction immunological methods with specific antibodies that...
The scaffolding adaptor protein p62/SQSTM1 (p62) has been shown to be an autophagy receptor that acts as a link between the ubiquitination and machineries. However, roles of p62 in human keratinocytes are not well understood. In this study, we show keratinocyte negatively regulates expression, which is essential for prevention excessive inflammation induction cathelicidin keratinocytes. Stimulation TLR2/6 or TLR4 primary robustly activated pathways up-regulated expression through NADPH...
Background Vitamin D plays an important role in the immune system, and its deficiency has been implicated various skin diseases, including atopic dermatitis psoriasis. Acne is a common inflammatory disease; however, association with vitamin remains unclear. Objectives We evaluated levels patients acne to determine effect of supplementation. Methods This study included 80 healthy controls. Serum 25-hydroxyvitamin (25(OH)D) were measured, demographic data collected. D-deficient treated oral...
The transglutaminase 3 enzyme is expressed during the late stages of terminal differentiation epidermis and in certain cell types hair follicle. thought to be critically involved cross-linking structural proteins formation cornified envelope, thereby contributing rigid structures that play vital roles shape determination and/or barrier functions. To explore mechanisms regulating expression gene (TGM3), 3.0 kilobase pairs sequences upstream from transcription start site were assessed for...
The use of botulinum toxin type A (BoNT) continues to expand. Some physicians have noted a face-lifting effect after intradermal injection BoNT, although the effects are controversial.To investigate in vitro BoNT on human dermal fibroblasts.The proliferation and toxic fibroblasts were measured. To understand mechanism collagen production fibroblasts, procollagen I carboxy-terminal peptide (PIP) was measured using enzyme-linked immunosorbent assay, monitored Western blotting. examine...
Imiquimod is known to exert its effects through Toll-like receptor 7 (TLR7) and/or TLR8, resulting in expression of proinflammatory cytokines and chemokines. Keratinocytes have not been reported constitutively express TLR7 the action imiquimod thought be mediated by adenine receptor, or TLR8. In this study, we revealed keratinocytes after calcium-induced differentiation. After addition calcium cultured keratinocytes, immunological responses induced imiquimod, such as activation NF-κB...
Nrf2 plays a role in protection of cells against oxidative stress and xenobiotic damage by regulating cytoprotective genes. In this study, we investigated the effect on melanogenesis normal human melanocytes (NHMCs). When NHMCs were transduced with recombinant adenovirus expressing Nrf2, melanin synthesis was significantly decreased. Consistent result, overexpression decreased expression tyrosinase tyrosinase-related protein 1. The inhibitory reversed Keap1, an intracellular regulator Nrf2....
Many alpha-helical proteins that form two-chain coiled coils possess a 13-residue trigger motif seems to be required for the stability of coil. However, as currently defined, is absent from intermediate filament (IF) protein chains, which nevertheless segmented coils. In present work, we have searched and identified two regions in IF chains are essential necessary formation coiled-coil molecules thus may function motifs. We made series point substitutions with keratin 5/keratin 14 system....
Mycobacterium ulcerans (MU), an environmental pathogen, causes Buruli ulcer, a severe skin disease. We hypothesized that epidermal keratinocytes might not be simple barrier, but play role during MU infection through pattern-recognition receptors expressed in keratinocytes. found keratinocyte Toll-like (TLRs) 2 and 4 Dectin-1 actively participate the innate immune response to MU, which includes internalization of bacteria, production reactive oxygen species (ROS), expression chemokines LL-37....
In this study, we investigated the expression and putative role of Sox9 in epidermal keratinocyte. Immunohistochemical staining showed that is predominantly expressed basal layer normal human skin epidermis, highly several diseases including psoriasis, cell carcinoma, keratoacanthoma squamous carcinoma. calcium-induced keratinocyte differentiation model, was decreased a time dependent manner. When overexpressed using recombinant adenovirus, growth enhanced, while differentiation-related...
Hair regression and balding are distressing concerns for an increasing number of people due to changes in lifestyle serious nutritional imbalances. Therapies treatment hair loss needed. Among potential therapeutics, adenosine has been suggested as a potent regulator growth. In this study, we investigated the effects on follicles dermal papilla (DP) cells, mechanism underlying action adenosine. organs, including DP that responsible production fibers by inducing maintaining growth phase...
Abstract Alopecia areata (AA) is a chronic, relapsing hair-loss disorder that considered to be T-cell-mediated autoimmune disease. Several animal models for AA have been created investigate the pathophysiology and screen effective therapeutic targets. As C3H/HeJ mice develop spontaneously in low frequency, novel model needed establish an AA-like condition faster more conveniently. In this study, we present non-invasive rodent avoids skin or lymph-node cell transfer. We simply injected...
BACKGROUND Laser and light-based therapies have been used successfully in the treatment of rosacea; however, evidence is lacking regarding efficacy radiofrequency (RF). OBJECTIVE This study evaluated RF rosacea compared with pulsed dye laser (PDL). METHODS Thirty patients (erythematotelangiectatic [ETR], n = 20; papulopustular [PPR], 10) were enrolled a randomized, controlled, split-face study. The treated on one side PDL other side. Each consisted 3 sessions at 4-week intervals followed up...