A5018172871- GABA and Rice Research
- Probiotics and Fermented Foods
- Food Quality and Safety Studies
- Protein Hydrolysis and Bioactive Peptides
- Healthcare and Venom Research
- Biopolymer Synthesis and Applications
- Medicinal Plants and Neuroprotection
- Nutrition, Health and Food Behavior
- Insect Utilization and Effects
- Biochemical effects in animals
- Pharmacological Effects of Natural Compounds
- Proteins in Food Systems
- Biochemical Analysis and Sensing Techniques
- Phytochemical and Pharmacological Studies
- Fungal Biology and Applications
- Enzyme-mediated dye degradation
- S100 Proteins and Annexins
- Polyamine Metabolism and Applications
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Pharmacological Effects and Assays
- Curcumin's Biomedical Applications
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Garlic and Onion Studies
Keimyung University
2014-2022
Korea Testing & Research Institute
2017
Daejeon University
2002-2015
Joongbu University
2014
Gyeongsang National University
2004-2005
Curcuma longa L. is a well-known medicinal plant that has been used for its anti-cancer, neuroprotective, and hepatoprotective effects. However, the neuroprotective effect of fermented C. (FCL) not reported. Therefore, in this study, effectiveness FCL regulation memory dysfunction was investigated two brain cell lines (rat glioma C6 murine microglia BV2) scopolamine-treated mice.C. powder by 5% Lactobacillus plantarum K154 containing 2% (w/v) yeast extract at 30 °C 72 h followed...
Substrates and enzymesFresh Holstein milk was obtained from a Gyeongsang National University livestock farm.Cheddar cheese manufactured according to the methods of Kosikowski (1982), CWP separated process contained 1.03% protein, 4.26% lactose, 0.7% ash 93.53% moisture (determined by AOAC method, 1990).Defatting demineralization were performed ultracentrifuge (Supra 25 K, Hanil Sci., Korea) dialysis (Sigma Co., USA), respectively.CWP adjusted pH 8.0 through addition 0.5 N NaOH then...
Background: HSP27 phosphorylation plays pivotal roles on F-actin polymerization and actin cytoskeleton organization.Results: The loss of hTid-1S expression was observed in lesional human psoriatic skin.Conclusion: binding with MK5 inhibits attenuates polymerization.Significance: lack correlates the aberrant organization skin. organization. Results: Conclusion: polymerization. Significance:
Enzymes and reagentsFresh goat's milk (Saanen; Ovis aegagrus) was purchased from Korean Medi-R Co. then defatted by ultrafiltration (Supra 25 K, Hanil Sci., Korea).The separation of CN performed according to the methods Sofia Malcate (2000).Trypsin (Bovine pancreas, activity 3.3 Anson units g -1 protein) Neutrase 0.8 (Bacillus subtilis, were Novo Nordisk A/S (Bagsvaerd, Denmark), Protease S stearothermophilis, 10,000 Papain W-40 (Carica
Background: Psoriasis is a chronic inflammatory skin disease that affects approximately 1~3% of the general population.Objective: We performed cDNA microarray analysis with using dendrimer labelling method to investigate gene expression profile in peripheral blood mononuclear cells (PBMCs) psoriatic patients.Methods: The 5 patients psoriasis and 8 control subjects were used analyses psoriasis.Results: identified 212 differentially expressed genes showed at least two-fold induction and/or...
Carbon monoxide (CO) is one of the primary components emissions from light-duty vehicles, and reportedly comprises 77% all pollutants emitted in terms concentration. Exposure to CO aggravates cardiovascular disease causes other health disorders. The study was aimed assess negative effects by injecting different amounts concentration directly human volunteers boarding car.Human were exposed concentrations 0, 33.2, 72.4 ppm, respectively during first test 30.3, 48.8 ppm second while seated...
In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as calcium-induced gene.In this study, further verified expression of NGAL cultured keratinocytes well several skin diseases.Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that was markedly increased differentiation vitro.However, our report, not detected normal tissue except hair...
A turmeric and roasted soybean flour mixture (TRSF) was successfully fermented in solid-state using Bacillus subtilis HA Lactobacillus plantarum K154. The co-fermentation optimized the (turmeric: RSF=1:1), 5% MSG, a 2-fold volume of water. TRSF by B. showed consistency 1.23 Pa.sn 3.57% (w/w) mucilage. co-fermented exhibited pH 5.94 1.94% acidity after second lactic acid fermentation. viable cell counts sp. were 4.50 × 107 CFU/g 8.00×109 CFU/g, respectively. In addition, higher GABA content...
경남 합천 일대에서 재배된 보리수나무(Elaeagnus umbellata Thunb.)의 생리활성을 부위별(열매, 잎, 줄기)로 평가한 결과는 다음과 같다. 항산화 특성을 측정한 결과, 총폴리페놀 함량이 높게 나타난 줄기(829.6 mg/g) 에탄올 추출물에서 DPPH 라디칼 소거능(ECSUB50/SUB=54.04 μg/mL)이 가장 우수하였다. 유리 아미노산 분석에서는 잎(6,179.12 mg/100 g) 추출물이 열매(378.88 g)와 줄기(1,211.69 추출물보다 높았으며, 열매의 proline, 잎의 asparagine 및 줄기의 GABA가 대표적인 아미노산인 것을 확인하였다. LC-MS/MS를 이용한 주요 페놀 성분 잎 gallic acid, 줄기 catechin의 HepG2 cell과 Chang cell의 암세포 증식억제효과를 결과 HepG2에 특이적으로 높은 억제 활성이 나타났다. 향후 보리수나무를 국내 우수 자원으로 활용하기 위해서는 추가로 다양한 암세포에...
This study was carried out to separate the calcium-binding protein derived from enzymatic hydrolysates of cheese whey protein.CWPs (cheese protein) heated for 10 min at 100°C were hydrolyzed by trypsin, papain W-40, protease S, neutrase 1.5 and pepsin, then properties hydrolysates, separation analysis ability investigated.The DH (degree hydrolysis) NPN (non nitrogen) heated-CWP commercial enzymes higher in trypsin than those other enzymes.In result SDS-PAGE (sodium dodecyl sulphate...
시판 우유를 이용하여 고초균과 젖산균의 혼합발효에 의한 호상 요구르트 제조에 미치는 영향을 관찰하였다. 우유 원료를 6시간 동안 고초균 발효를 통해서 생균수가 초기 <TEX>$6.0{\times}10^6$</TEX> CFU/mL에서 <TEX>$2.5{</TEX><TEX>\times}10^8$</TEX> CFU/mL로 증가되었다. 2차 젖산균 발효시에 생균수 증가 및 산 생성능이 촉진되었으며, 발효 1일 후에 <TEX>$3.03{\times}10^9$</TEX> CFU/mL을 나타내었으며, 고초균은 <TEX>$4.67{\times}10^5$</TEX> 감소하였다. 단백질 카제인은 1시간 1차 발효에 의해서 급격하게 가수분해되어 저분자 펩타이드로 전환되었으며, 유청분리가 최소화되면서 커드형성이 우수하였다. 3시간까지 젖산균에 의해 커드형성능이 양호하였으며, 그 이상의 발효는 우유의 커드형성을 지연시켰다. 특히 4시간 발효물은 심한 유청분리 현상과 함께 tyrosine 함량이...
Objectives : This research investigates the single oral dose toxicity of ACM in SD rats. Methods ACMs were administered to female and male rats, as an 5000 mg/kg. Animals monitored for mortality changes body weight, clinical signs gross observation during 14 days after dosing, upon necropsy. Results We could not find any mortality. Compared with control group, significant weight change was observed experimental group. First day administration, compound-colored stool all After second more...
Objective : The aim is to examine the effect of Soyeum Pharmacopuncture (SPP) on collagen-induced arthritis (CIA) in DBA/1OlaHsd mice. Methods To determine SPP chronic IFNlammatory joint disease, we induced CIA mice by immunization with bovine type II collagen. Animals were treated intraperitoneal injection doses 2 mg/kg SPP, beginning 3 days before expected onset disease symptoms. Inhibitory Effects observed serum levels TNF-, and IFN-,,, or cell proliferation spleen culture histological...
Toxicodendron vernicifluum extract (TVE) was co-fermented by Saccharomyces cerevisiae and Lactobacillus plantarum EJ2014 to produce higher content of gamma-aminobutyric acid (GABA). The TVE cultured at 30°C for 2 days under static conditions with sucrose 5%, yeast 1%, starter 1%. second lactic fermentation after the primary first performed 7 adding monosodium L-glutamate (MSG) 2.5%, lactose 2.5% bacteria (LAB) fermented showed pH 4.6, 0.1% acidity viable cell counts S. 7.63 log CFU/mL. After...
Objectives : To provide information on the safety of ACM, we carried out a 13-week repeated oral dose toxicity and 4-week recovery test ACM in Sprague-Dawley rats. Methods Female male rats were treated with doses 800, 2000, 5000 mg/kg. The was administered for 13 weeks. Mortality, clinical signs, body weight changes, food consumption, ophthalmologic findings, urinalysis, hematological biochemical parameters, gross organ weights histological markers monitored during study period. Moreover, 4...