The scaffolding adaptor protein p62/SQSTM1 (p62) has been shown to be an autophagy receptor that acts as a link between the ubiquitination and machineries. However, roles of p62 in human keratinocytes are not well understood. In this study, we show keratinocyte negatively regulates expression, which is essential for prevention excessive inflammation induction cathelicidin keratinocytes. Stimulation TLR2/6 or TLR4 primary robustly activated pathways up-regulated expression through NADPH...

Targeted disruption of STAT3 function has proven to be a useful cancer therapeutic approach by inducing apoptotic cell death. Cucurbitacin is currently under development as small molecule inhibitor trigger death in many cancers. Here, we systematically studied the molecular mechanisms underlying cucurbitacin-induced death, particular involvement autophagy. Treatment with cucurbitacin resulted non-apoptotic caspase-independent manner. Notably, enhanced excessive conversion lipidated LC3...

[Purpose] The aim of this study was to investigate the effects aerobic exercise training on a high fat diet (HFD)-induced fatty liver and its metabolic complications in C57BL/6 mice. [Methods] Mice at 5-month old (n = 30) were randomly assigned standard chow (SC + CON, n 10) high-fat (HFD, 20), they subjected SC HFD, respectively, for 23-week. After 15-week mice HFD group further (HFD or EX, groups. EX treadmill running during last 8-week 23-week course. Outcomes included hepatic steatosis,...

The N-degron pathway is a proteolytic system in which the N-terminal degrons (N-degrons) of proteins, such as arginine (Nt-Arg), induce degradation proteins and subcellular organelles via ubiquitin-proteasome (UPS) or macroautophagy/autophagy-lysosome (hereafter autophagy). Here, we developed chemical mimics Nt-Arg pharmaceutical means to targeted intracellular bacteria autophagy, Salmonella enterica serovar Typhimurium (S. Typhimurium), Escherichia coli, Streptococcus pyogenes well...

Abstract Objective Spirituality is what gives people meaning and purpose in life, it has been recognized as a critical factor patients’ well-being, particularly at the ends of their lives. Studies have demonstrated relationships between spirituality patient-reported outcomes such quality life mental health. Although number studies suggested that spiritual belief can be associated with mortality, results are inconsistent. We aimed to determine whether was related survival advanced cancer...

[Purpose] The purpose of the study was to investigate effect 12-week walking exercise on abdominal fat, insulin resistance and serum cytokines in obese women. [Methods] Following baseline measurements, women (N = 20) who met obesity criterion BMI at 25 kg/m2 or greater were randomly assigned control (n 10) groups 10). Women group participated a (with an intensity 50-60% predetermined VO2max, frequency 3 days per week duration 50-70 minutes targeting 400 kcal energy expenditure session) for...

Objectives In Uganda, infection-related cancers have made the greatest contribution to cancer burden in past; however, from lifestyle-related has increased recently. Using Kampala Cancer Registry data, we projected incidence of top five cancers, namely, Kaposi sarcoma (KS), cervical, breast and prostate cancer, non-Hodgkin’s lymphoma (NHL) Uganda. Design Trend analysis registry data. Setting Registry, Main outcome measure data 2001 2015 were used 2030. Population obtained Uganda Bureau...

The joinpoint regression model (JRM) is used to describe trend changes in many applications and relies on the detection of joinpoints (changepoints). However, existing methods, namely, grid search (GS)‐based are computationally demanding, hence, maximum number computable limited. Herein, we developed a genetic algorithm‐based (GAJP) which an explicitly decoupled computing procedure for optimization embed binary algorithm into JRM optimal detection. combinations were represented as...

This study examined the variability in length of stay (LOS) and daily medical expenses (DME) for hypertension pneumonia inpatient care. Using 10 years National Health Insurance Service data (2010-2019), a multilevel analysis assessed at patient institutional levels. During period, mean LOS decreased, whereas DME increased both pneumonia. Institutional level during demonstrating greater than that For conditions, institutional-level was more marked smaller institutions (hospitals clinics)...

Given its importance in various cellular processes, autophagy has attracted growing attention as a promising therapeutic target many human diseases. Herein, we report the construction of high-content screening platform for discovery modulators via monitoring lipid droplets late-stage marker autophagy. Notably, our discriminates effects chemical on autophagic flux without additional transfection, washing, or fixation. This approach allows simultaneous identification completely different types...

Abstract The Arg/N-degron pathway, which is involved in the degradation of proteins bearing an N-terminal signal peptide, connected to p62/SQSTM1-mediated autophagy. However, impact molecular link between N-degron and autophagy pathways largely unknown context systemic inflammation. Here, we show that chemical mimetics Nt-Arg pathway (p62 ligands) decreased mortality sepsis inhibited pathological inflammation by activating mitophagy immunometabolic remodeling. p62 ligands alleviated a mouse...

The global incidence of Mycobacterium abscessus (Mabc), a rapidly growing nontuberculous mycobacterial strain that causes treatment-refractory pulmonary diseases, is increasing. Despite this, the host factors allow for protection against infection are largely unknown. In this study, we found sirtuin 3 (SIRT3), mitochondrial protein deacetylase, plays critical role in defense Mabc infection. decreased SIRT3 and upregulated oxidative stress macrophages. deficiency led to increased bacterial...

As the onset of cancer recurrence is not explicitly recorded in electronic health record (EHR), a high volume manual chart review required to detect recurrence. This study aims develop an automatic rule-based algorithm for detecting ovarian (OC) on basis minimally preprocessed EHR data.

Abstract Ubiquitin regulatory X (UBX) domain-containing protein 6 (UBXN6) is an essential cofactor for the activity of valosin-containing p97, adenosine triphosphatase associated with diverse cellular activities. Nonetheless, its role in cells innate immune system remains largely unexplored. In this study, we report that UBXN6 upregulated humans sepsis and may serve as a pivotal regulator inflammatory responses via activation autophagy. Notably, upregulation patients was negatively...

Despite the progress of advanced target therapeutic agents and immune checkpoint inhibitors, EGFR-TKI resistance is still one biggest obstacles in treating lung cancer. Clinical studies with autophagy inhibitors are actively underway to overcome drug resistance.We used PC9, PC9/GR, HCC827/GR cell lines evaluate activation resistance. Chloroquine was applied as an autophagic blocker verteporfin utilized a YAP inhibitor.In this study, we tried reveal effect adaptor p62 which accumulated by...

This study aimed to estimate the prevalence of pre-existing lung diseases in patients with cancer compared people without and examine association between income levels diseases.Data on (case) general population (non-cancer controls) matched by age, sex region were obtained from Korea National Health Insurance Service-National Information Database (n=51 586). premiums divided into quintiles medicaid patients. Conditional logistic regression models used risk cancer. The relationship level...

Risk factor modification is key to preventing subsequent cardiac events after a heart attack. This study was designed investigate the disparity between preventive guidelines and clinical practice among smoking patients.The carried out in smokers admitted with myocardial infarction (MI). A total of 275 patients who had been regularly followed for over one year MI were randomly selected enrolled this study. We investigated changes behavior adherence rate ACC/AHA Guidelines secondary prevention...

Nontuberculous mycobacterial pulmonary infection is often aggravated due to antibiotic resistance issues. There a need for development of new drugs inducing both host immune responses and antimicrobial activities. This study shows that the rufomycins 4/5/6/7 (Rufomycin 4–7), which targets ClpC1 as subunit caseinolytic protein complex ClpC1/ClpP1/ClpP2 mycobacteria, exhibits dual effect in innate defense vivo activities against rough morphotype Mycobacterium abscessus (Mabs-R), clinically...

Abstract Little is known about patients reporting extremely poor health-related quality of life (HRQoL). This study targeted population with inferior HRQoL and examined their problems experienced dimensions, impacts different morbidities on these problems. Data were obtained from a population-based survey in Korea. was measured by EQ-5D questionnaire low-HRQoL defined as individuals whose utility score among the lowest 5% total population. Logistic regression models used to evaluate impact...

OBJECTIVES: Improved life expectancy has increased the prevalence of older adults living with multimorbidity which likely deteriorates their health-related quality (HRQoL). However, relatively little is known about patterns and relationships by HRQoL status in adults.METHODS: Individuals aged 65 or from Korea National Health Nutrition Examination Survey V-VII (2010-2018) were analyzed. was assessed EuroQoL-5 dimensions questionnaire categorized as poor, normal, good. The impact on evaluated...

Abstract Background Cancer patients’ prognoses are complicated by comorbidities. Prognostic prediction models with inappropriate comorbidity adjustments yield biased survival estimates. However, an appropriate claims-based risk assessment method remains unclear. This study aimed to compare methods used capture comorbidities from claims data and predict non-cancer mortality risks among cancer patients. Methods Data were obtained the National Health Insurance Service-National Sample Cohort...

Background: The aim of the present study was to characterize heart rate dynamics sinus bradycardia (SB) from node dysfunction (SND) using non-linear dynamical system analysis. No data are yet available on how change in presence SND. Methods and Results: Conventional time frequency domain analysis, short- (DFAα1) intermediate-term fractal scaling exponent (DFAα2), approximate entropy (ApEn) sample (SampEn) were calculated 60-min RR interval SB 24-h ambulatory electrocardiograms 110 patients:...

With the remarkable development of digital technology, wireless communication, and smartphone device, there have been made various transformations in a wide range technology. In addition, service platforms appear as well, which gradually lead to interest content portal sites an open cooperative platform. However, practical use contents has not well produced profits by using its strength properly so far. Thus, I investigated impact usage way platform example successful case. For this purpose,...

Venous thromboembolism (VTE) is a hospital-associated severe complication that may adversely affect patient prognosis. In this study, we evaluated the incidence of VTE and its risk factors in patients with epithelial ovarian cancer (EOC).We retrospectively analyzed electronic health record data 1268 EOC who received primary treatment at National Cancer Center, Korea between January 2007 December 2017 to identify developed VTE. Demographic, clinical, surgical characteristics these were...