A5089843095- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- interferon and immune responses
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- Natural product bioactivities and synthesis
- Protein Kinase Regulation and GTPase Signaling
- CRISPR and Genetic Engineering
- Nitric Oxide and Endothelin Effects
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sirtuins and Resveratrol in Medicine
- Hearing, Cochlea, Tinnitus, Genetics
- Endoplasmic Reticulum Stress and Disease
- PI3K/AKT/mTOR signaling in cancer
- Biological Stains and Phytochemicals
- Ion Transport and Channel Regulation
- Pharmacological Effects of Natural Compounds
- Antimicrobial Peptides and Activities
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Hepatitis B Virus Studies
Chungnam National University
2015-2025
Regional Cancer Center
2009-2012
Daejeon University
2012
Indiana University School of Medicine
2012
Seoul National University Bundang Hospital
2007
National Institutes of Health
2000-2006
Center for Cancer Research
2003-2006
City of Hope
2005
Beckman Research Institute
2005
National Cancer Institute
2003
The mechanism of tumor necrosis factor (TNF)-induced nonapoptotic cell death is largely unknown, although the TNF-induced apoptosis has been studied extensively. In wild-type mouse embryonic fibroblast cells under a caspase-inhibited condition, TNF effectively induced that morphologically resembled necrosis. this study, we utilized gene knockout fibroblasts and found receptor (TNFR) I mediates necrotic death, RIP, FADD, TRAF2 are critical components signaling cascade death. Inhibitors NF-κB...
Targeted disruption of STAT3 function has proven to be a useful cancer therapeutic approach by inducing apoptotic cell death. Cucurbitacin is currently under development as small molecule inhibitor trigger death in many cancers. Here, we systematically studied the molecular mechanisms underlying cucurbitacin-induced death, particular involvement autophagy. Treatment with cucurbitacin resulted non-apoptotic caspase-independent manner. Notably, enhanced excessive conversion lipidated LC3...
The transcription factor NF-kappaB is activated when cells are exposed to genotoxic stress. It has been suggested that DNA damage will trigger a cytoplasmic signaling leads the activation of IKK and NF-kappaB, but components upstream have not yet identified. Here we report receptor interacting protein, RIP, component, essential for by damage. Also, our findings suggest this mediated autocrine or TNF-R1 pathway. In wild-type fibroblasts, induced agents such as adriamycin, campthothecin,...
Mycobacterium tuberculosis (Mtb) infection leads to the induction of apoptotic response, which is associated with bacilli killing. The early secreted mycobacterial antigen ESAT‐6 Mtb has been shown induce apoptosis in human macrophages and epithelial cells. In present study, we demonstrate that stimulation A549 cells by induces endoplasmic reticulum (ER) stress response. We observed treatment increases intracellular Ca 2+ concentration, results ROS accumulation, therefore onset ER...
The N-degron pathway is a proteolytic system in which the N-terminal degrons (N-degrons) of proteins, such as arginine (Nt-Arg), induce degradation proteins and subcellular organelles via ubiquitin-proteasome (UPS) or macroautophagy/autophagy-lysosome (hereafter autophagy). Here, we developed chemical mimics Nt-Arg pharmaceutical means to targeted intracellular bacteria autophagy, Salmonella enterica serovar Typhimurium (S. Typhimurium), Escherichia coli, Streptococcus pyogenes well...
Abstract Objective Nuclear factor‐κB (NF‐κB) has been implicated as a therapeutic target for the treatment of rheumatoid arthritis (RA). The purpose this study was to determine whether A20, universal inhibitor NF‐κB, might have antiarthritic effects. Methods An adenovirus containing A20 complementary DNA (AdA20) used deliver human fibroblast‐like synoviocytes (FLS) in vitro well mice with collagen‐induced (CIA) vivo via intraarticular injection into ankle joints bilaterally. Results In...
Abstract TNF receptor 1 can activate signaling pathways leading to the activation of NF-κB. A20, an NF-κB-inducible protein, negatively regulates these and acts as anti-inflammatory mediator. Therefore, A20 is viewed a potential therapeutic target for inflammatory disease. In this study, we examined effect on OVA-induced allergic airway inflammation model in mice. We used adenovirus containing cDNA (Ad-A20) that was delivered intratracheally before OVA challenge. Single administration Ad-A20...
Leucine zipper/EF hand-containing transmembrane-1 (LETM1) is a mitochondrial inner membrane protein that was first identified in Wolf-Hirschhorn syndrome, and deleted nearly all patients with the syndrome. LETM1 encodes for human homologue of yeast Mdm38p, which mitochondria-shaping unclear function. Here, we describe LETM1-mediated regulation ATP production biogenesis. We show overexpression can induce necrotic cell death HeLa cells, reduces biogenesis production. acts as an anchor...
Abstract ISG15 is an interferon-stimulated ubiquitin-like protein (UBL) with multifaceted roles as a posttranslational modifier in conjugation (ISGylation). However, the mechanistic consequences of ISGylation cancer have not been fully elucidated, largely due to lack knowledge on target repertoire. Here, we identified SIRT1, nicotinamide adenine dinucleotide (NAD + )-dependent deacetylase, new for ISGylation. SIRT1 impairs association its negative regulator, deleted breast 1 (DBC1), which...
Midazolam, a benzodiazepine, has hypnotic effect and is widely used as sedative. The role of midazolam in activation macrophages during sepsis not known. aim this study was to evaluate the antiinflammatory actions cultured macrophages.Using macrophage cell line, RAW264.7 cells, on proinflammatory mediators mitogen-activated protein kinase measured by Western blot. Nuclear factor-kappaB (NF-kappaB) translocation p65 subunit NF-kappaB using luciferase assay immunocytochemistry. Superoxide...
3-Phosphoinositide-dependent protein kinase-1 (PDK1) appears to play a central regulatory role in many cell signalings between phosphoinositide-3 kinase and various intracellular serine/threonine kinases. In resting cells, PDK1 is known be constitutively active further activated by tyrosine phosphorylation (Tyr(9) Tyr(373/376)) following the treatment of with insulin or pervanadate. However, little about mechanisms for this additional activation PDK1. Here, we report that SH2 domain Src,...
Objective. Resveratrol is a naturally occurring polyphenol, which possesses chemotherapeutic potential through its ability to trigger apoptosis. The objective of this study was investigate the major determinant for apoptotic cell death induction by resveratrol in fibroblast-like synoviocytes (FLS) derived from patients with RA.
Abstract The senescence of human mesenchymal stem cells (hMSCs) causes disruption tissue and organ maintenance, is thus an obstacle to cell-based therapies for disease. Although some researchers have studied changes in the characteristics hMSCs (decreases differentiation ability self-renewal), comparing young old ages, mechanisms cell not yet been defined. In this study, we developed a growth curve bone marrow derived MSCs (hBMSCs) which into hyperbolic state after passage number 7....
This study characterized the upstream signalling molecules involved in extracellular signal-regulated kinase (ERK) 1/2 activation and determined their effects on differential tumour necrosis factor (TNF)-α expression by monocytes/macrophages infected with virulent or avirulent mycobacteria. The Mycobacterium tuberculosis (MTB) strain H37Ra (MTBRa) induced higher levels of ERK MAPK (MEK)1 and, subsequently, TNF-α human primary monocytes monocyte-derived macrophages, as compared MTB H37Rv...