A5087022387- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Chemokine receptors and signaling
- Infectious Diseases and Tuberculosis
- Allergic Rhinitis and Sensitization
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- Proteoglycans and glycosaminoglycans research
- Parasites and Host Interactions
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Immune cells in cancer
- interferon and immune responses
- Diagnosis and treatment of tuberculosis
- Fibroblast Growth Factor Research
- Parasite Biology and Host Interactions
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Retinoids in leukemia and cellular processes
- Helminth infection and control
- Immunodeficiency and Autoimmune Disorders
- Mast cells and histamine
- Nephrotoxicity and Medicinal Plants
University of London
2020-2025
Brunel University of London
2025
Universidad de Londres
2025
WonKwang Health Science University
2011-2024
London School of Hygiene & Tropical Medicine
2013-2023
Seoul St. Mary's Hospital
2023
Catholic University of Korea
2023
Pfizer (United States)
2021-2023
Halozyme Therapeutics (United States)
2017-2022
Seoul National University Bundang Hospital
2021
Rationale: Contacts of patients with tuberculosis (TB) constitute an important target population for preventive measures because they are at high risk infection Mycobacterium and progression to disease.Objectives: We investigated biosignatures predictive ability incident TB.Methods: In a case–control study nested within the Grand Challenges 6-74 longitudinal HIV-negative African cohort exposed household contacts, we employed RNA sequencing, PCR, pair ratio algorithm in training/test set...
Background. Accurate assessment of treatment efficacy would facilitate clinical trials new antituberculosis drugs. We hypothesized that early alterations in peripheral immunity could be measured by gene expression profiling tuberculosis patients undergoing successful conventional combination treatment.
Abstract Antigen-specific, MHC-restricted αβ T cells are necessary for protective immunity against Mycobacterium tuberculosis , but the ability to broadly study these responses has been limited. In present study, we used single-cell and bulk cell receptor (TCR) sequencing GLIPH2 algorithm analyze M. -specific sequences in two longitudinal cohorts, comprising 166 individuals with infection who progressed either ( n = 48) or controlled 118). We found 24 groups similar TCR-β sequences,...
New biomarkers of tuberculosis (TB) risk and disease are critical for the urgently needed control ongoing TB pandemic. In a prospective multisite study across Subsaharan Africa, we analyzed metabolic profiles in serum plasma from HIV-negative, TB-exposed individuals who either progressed to 3-24 months post-exposure (progressors) or remained healthy (controls). We generated trans-African biosignature TB, which identifies future progressors both on blinded test samples external data sets...
Abstract Improved tuberculosis diagnostics and tools for monitoring treatment response are urgently needed. We developed a robust simple, PCR-based host-blood transcriptomic signature, RISK6, multiple applications: identifying individuals at risk of incident disease, as screening test subclinical or clinical tuberculosis, treatment. RISK6 utility was validated by blind prediction using quantitative real-time (qRT) PCR in seven independent cohorts. Prognostic performance significantly...
Abstract Background Cyclin-dependent kinase 4/6 inhibitor (CDK4/6) therapy plus endocrine (ET) is an effective treatment for patients with hormone receptor-positive/human epidermal receptor 2-negative metastatic breast cancer (HR+/HER2− MBC); however, resistance common and poorly understood. A comprehensive genomic transcriptomic analysis of pretreatment post-treatment tumors from receiving palbociclib ET was performed to delineate molecular mechanisms drug resistance. Methods Tissue...
ABSTRACT Although the 38-kDa glycolipoprotein of Mycobacterium tuberculosis H37Rv is known to evoke prominent cellular and humoral immune responses in human (TB), little information about intracellular regulatory mechanisms involved antigen (Ag)-induced host responses. In this study, we found that purified activates mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase 1/2 [ERK1/2] p38) induces tumor necrosis factor alpha (TNF-α) interleukin 6 (IL-6) monocytes. When...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating condition with unknown aetiology, unclear pathophysiology and no diagnostic test or biomarker available. Many patients report their ME/CFS began after an acute infection, subsequent increased frequency of infections, such as colds influenza, common. These factors imply altered immunological status exists in ME/CFS, at least proportion patients, yet previous studies peripheral immunity have been discrepant...
We previously demonstrated that Mycobacterium tuberculosis (M. tbc)-induced interleukin (IL)-12 expression is negatively regulated by the phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (ERK) 1/2 pathways in human monocyte-derived macrophages (MDMs). To extend these studies, we examined nature of involvement toll-like receptors (TLRs) intracellular signalling downstream from PI3K M. tbc-induced IL-23 MDMs. Akt activation were essentially dependent on TLR2....
Human melanocytes infected with Ki-MSV or Ha-MSV, but not amphotropic MuLV, undergo a series of transformation-related changes that are characteristic malignant melanoma. These (a) expression Ia antigens, in particular DP, DQ, and DR class II histocompatibility gene products, (b) transformed morphology ability to grow soft agar, (c) 5-10-fold increase the cell surface GD3 ganglioside. However, other characteristics melanoma, such as independence from specific growth factors loss adenosine...
The cell wall skeleton of Mycobacterium bovis Bacillus Calmette-Guerin (BCG/CWS) is an effective anti-tumor immunotherapy agent. Here, we demonstrate that BCG/CWS has a radiosensitizing effect on colon cancer cells through the induction autophagic death. Exposure HCT116 to before ionizing radiation (IR) resulted in increased death caspase-independent manner. Treatment with plus IR autophagy cells. Either inhibitor 3-methyladenine or knockdown Beclin-1 Atg7 significantly reduced tumor induced...
Background and purpose We investigated the effect of celecoxib, a selective inhibitor cyclo‐oxygenase 2, in patients with intracerebral hemorrhage ( ICH ). Methods conducted multicenter, randomized, controlled, open blinded end‐point trial 44 Korean 18 years or older within 24 h onset. The intervention group n = 20) received celecoxib (400 mg twice day) for 14 days. control 24) standard medical treatment . primary was number change volume perihematomal edema PHE ) from 1st to 7th ± 1 day...
S100A8 and S100A9 are important proteins in the pathogenesis of allergy.Asthma is an allergic lung disease, characterized by bronchial inflammation due to leukocytes, bronchoconstriction, allergen-specific IgE.In this study, we examined role interaction cytokine release from epithelial cells, with constitutive apoptosis neutrophils.S100A8 induce increased secretion neutrophil survival cytokines such as MCP-1, IL-6 IL-8.This suppressed TLR4 inhibitor), LY294002, AKT inhibitor, PD98059,...
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex chronic condition affecting multiple body systems, with unknown cause, unclear pathogenesis mechanisms, and fluctuating symptoms which may lead to severe debilitation. It frequently reported have been triggered by an infection, but there are no clear differences in exposure to, or seroprevalence of, any particular viruses between people ME/CFS healthy individuals. However, herpes repeatedly hypothesized underlie the...
This study characterized the upstream signalling molecules involved in extracellular signal-regulated kinase (ERK) 1/2 activation and determined their effects on differential tumour necrosis factor (TNF)-α expression by monocytes/macrophages infected with virulent or avirulent mycobacteria. The Mycobacterium tuberculosis (MTB) strain H37Ra (MTBRa) induced higher levels of ERK MAPK (MEK)1 and, subsequently, TNF-α human primary monocytes monocyte-derived macrophages, as compared MTB H37Rv...
Background and objectives: The genetic determinants for developing TB or having recurrent are unknown. present study investigated the relationship between susceptibility to tuberculosis human tumour necrosis factor‐α (TNF‐α) interleukin‐10 genes (IL‐10). Methods: A case–control was conducted using two groups of cases—newly diagnosed (N‐TB) (R‐TB)—and a control group. Results: One hundred seventeen healthy controls, 80 newly patients 65 with were enrolled. There no significant difference in...
The UK ME/CFS Biobank was launched in August 2011 following extensive consultation with professionals and patient representatives. bioresource aims to enhance research on myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), related pathophysiology, biomarkers therapeutic approaches. cohort includes 18–60 year olds, encompassing 284 clinically-confirmed cases, 60 neurologist-diagnosed multiple sclerosis (MS) 135 healthy individuals. contains blood samples, aliquoted into serum,...
Background. Currently, there are no tools to accurately predict tuberculosis relapse. This study aimed determine whether patients who experience relapse have different immune responses mycobacteria in vitro than remain cured for 2 years. Methods. Patients with an initial episode of pulmonary were recruited South Africa. Diluted blood, collected at diagnosis and after 4 weeks treatment, was cultured live Mycobacterium 6 days, cellular RNA frozen. Gene expression samples from 10 subsequently...
PEGylated human hyaluronidase (PEGPH20) enzymatically depletes hyaluronan, an important component of the extracellular matrix, increasing delivery therapeutic molecules. Combinations chemotherapy and PEGPH20, however, have been unsuccessful in Phase III clinical trials. We hypothesize that by tumor oxygenation improving vascular patency perfusion, PEGPH20 will also act as a radiosensitization agent.The effect on radiation treatment was analyzed with respect to growth, survival time, p02,...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating but poorly-understood disease. ME/CFS symptoms can range from mild to severe, and include immune system effects alongside incapacitating post-exertional disease exacerbation. In this study, we examined immunological profiles of people living with by flow cytometry, focusing on cytotoxic cells, determine whether mild/moderate (n= 43) or severe (n=53) expressed different markers. We found that had increased...
ABSTRACT Tuberculosis (TB) is the leading cause of death from a single infectious agent in Korea. In this study, we compared proteins present culture filtrates Mycobacterium tuberculosis strain K, which dominant clinical isolate Korea, with those M. H37Rv. Several differences expression were detected between two strains for molecular mass <20 kDa. ESAT-6, HSP-X, and CFP-10 found to be abundantly expressed K by liquid chromatography-electrospray ionization-time flight spectrometry. The...