A5041535380- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Antifungal resistance and susceptibility
- Infectious Diseases and Tuberculosis
- Fungal Infections and Studies
- Immune Cell Function and Interaction
- DNA and Nucleic Acid Chemistry
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- vaccines and immunoinformatics approaches
- CRISPR and Genetic Engineering
- FOXO transcription factor regulation
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Phagocytosis and Immune Regulation
- Fungal and yeast genetics research
- Melanoma and MAPK Pathways
- Cancer-related gene regulation
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Immune responses and vaccinations
Daegu Health College
2012-2024
UNC Lineberger Comprehensive Cancer Center
2019-2021
University of North Carolina at Chapel Hill
2019-2021
Duke Medical Center
2010-2020
Duke University Hospital
2010-2020
Duke University
2017-2020
London School of Hygiene & Tropical Medicine
2013-2015
Institute of Neuroimmunology of the Slovak Academy of Sciences
2012
Yonsei University
2003-2008
Background. Accurate assessment of treatment efficacy would facilitate clinical trials new antituberculosis drugs. We hypothesized that early alterations in peripheral immunity could be measured by gene expression profiling tuberculosis patients undergoing successful conventional combination treatment.
High levels of transcription in Saccharomyces cerevisiae are associated with increased genetic instability, which has been linked to DNA damage. Here, we describe a pGAL-CAN1 forward mutation assay for studying transcription-associated mutagenesis (TAM) yeast. In wild-type background no alterations repair capacity, ≈50% mutations that arise the CAN1 gene under high-transcription conditions deletions 2–5 bp. Furthermore, characteristic TAM localize discrete hotspots coincide 2–4 copies tandem...
Significance The incidence of infections due to fungal pathogens has dramatically increased in human populations with weakened or suppressed immune systems. Understanding how organisms rapidly adapt during infection enhance virulence and evolve drug resistance is important for developing effective treatments. We find that transposon mobilization the pathogen Cryptococcus causes genomic mutations a murine model promotes antifungal drugs vitro. Thermotolerance key determinant pathogenic fungi...
Background. Currently, there are no tools to accurately predict tuberculosis relapse. This study aimed determine whether patients who experience relapse have different immune responses mycobacteria in vitro than remain cured for 2 years. Methods. Patients with an initial episode of pulmonary were recruited South Africa. Diluted blood, collected at diagnosis and after 4 weeks treatment, was cultured live Mycobacterium 6 days, cellular RNA frozen. Gene expression samples from 10 subsequently...
Abstract Genome integrity and genome engineering require efficient repair of DNA double-strand breaks (DSBs) by non-homologous end joining (NHEJ), homologous recombination (HR), or alternative end-joining pathways. Here we describe two complementary methods for marker-free quantification DSB pathway utilization at Cas9-targeted chromosomal DSBs in mammalian cells. The first assay features the analysis amplicon next-generation sequencing data using ScarMapper, an iterative break-associated...
Topoisomerase I (Top1) resolves supercoils by nicking one DNA strand and facilitating religation after torsional stress has been relieved. During its reaction cycle, Top1 forms a covalent cleavage complex (Top1cc) with the nicked DNA, this intermediate can be converted into toxic double-strand break (DSB) during replication. We previously reported that Top1cc trapping in yeast increases DSB-independent, short deletions at tandemly repeated sequences. In current study, we report type of...
Ribonucleotides are the most abundant non-canonical component of yeast genomic DNA and their persistence is associated with a distinctive mutation signature characterized by deletion single repeat unit from short tandem repeat. These events dependent on topoisomerase I (Top1) initiated Top1 incision at relevant ribonucleotide 3'-phosphodiester. A requirement for re-ligation activity led us to propose sequential cleavage model Top1-dependent mutagenesis ribonucleotides. Here, we test key...
Mycobacterium tuberculosis (MTB) resides and replicates inside macrophages.In our previous report, we reported that CD8+ T cell-mediated immune responses specific for the peptide derived from MTB RNA polymerase beta-subunit (RpoB 127-135 ) could be induced in TB patients expressing HLA-A*0201 subtype.In order to examine whether RpoB cells can recognize infected macrophages vitro, cell lines were generated peripheral blood mononuclear (PBMCs) of healthy subjects by vitro immunization...
Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is causative agent of bovine tuberculosis, contagious disease animals.Mycobacterial culture gold standard for diagnosing but this technique laborious and time-consuming.In present study, performance SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains bovis field isolates collected from animals.The MPT64 test produced positive results 95.5% (63/66) cattle 97.9%...
Background Biomarkers predicting tuberculosis treatment response and cure would facilitate drug development. This study investigated expression patterns of the co-stimulation molecule NKG2D in human to determine its potential usefulness as a host biomarker efficacy. Methods Tuberculosis patients (n = 26) were recruited Lahore, Pakistan, at diagnosis followed up during treatment. Household contacts 24) also recruited. was measured by qRT-PCR RNA samples both ex vivo following overnight...
Abstract DNA Polymerase Theta mediates an end joining pathway (TMEJ) that repairs chromosome breaks. It requires resection of broken ends to generate long, 3’ single stranded tails, annealing complementary sequence segments (microhomologies) in these followed by microhomology-primed synthesis sufficient resolve ends. The means which microhomologies are identified is thus a critical step this pathway, but not understood. Here we show scanning mechanism initiated from the terminus and favoring...
Tuberculosis (TB) remains a major health problem worldwide.TB depends not only on the characteristics of Mycobacterium tuberculosis (MTB) but also genetic susceptibility infected patients.Recent studies have suggested that FOXO3 play an important role in human immune associated disorder, such as TB.It was previously reported variants with risk TB Chinese population.In this study, we confirm whether polymorphism gene, which Chinese, is reproduced Korean population.Of 154 SNPs were extracted...