A5012791367- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Redox biology and oxidative stress
- Hematopoietic Stem Cell Transplantation
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Vitamin D Research Studies
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- NF-κB Signaling Pathways
- Heat shock proteins research
- interferon and immune responses
- Inflammasome and immune disorders
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- Reproductive System and Pregnancy
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Liver physiology and pathology
- Adipokines, Inflammation, and Metabolic Diseases
- Macrophage Migration Inhibitory Factor
- TGF-β signaling in diseases
- Glutathione Transferases and Polymorphisms
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Korea University of Science and Technology
2014-2023
Convergence
2018
Genomics (United Kingdom)
2010
Chungnam National University
2008
Institute of Neuroimmunology of the Slovak Academy of Sciences
2005
Virginia Commonwealth University Medical Center
1990-1991
University of Alabama
1987-1988
Abstract As a result of identifying the regulatory proteins thioredoxin (TRX), murine homologue for human vitamin D3 up-regulated protein 1 (VDUP1) was identified from yeast two-hybrid screen. Cotransfection into 293 cells and precipitation assays confirmed that mouse VDUP1 (mVDUP1) bound to TRX, but it failed bind Cys32 Cys35 mutant suggesting redox-active site is critical binding. mVDUP1 ubiquitously expressed in various tissues located cytoplasm. Biochemical analysis showed inhibited...
The redox-dependent inhibition of thioredoxin (TRX) by thioredoxin-interacting protein (TXNIP) plays a pivotal role in various cancers and metabolic syndromes. However, the molecular mechanism this regulation is largely unknown. Here, we present crystal structure TRX–TXNIP complex demonstrate that TRX TXNIP mediated an intermolecular disulphide interaction resulting from novel bond-switching mechanism. Upon binding to TRX, undergoes structural rearrangement involves switching head-to-tail...
STUDY QUESTIONIs the decreased natural killer (NK) cell cytolytic activity in peritoneal fluid (PF) of endometriosis patients due to primary cytokine activity?
Natural killer (NK) cells play important roles in immune surveillance. However, the tumor microenvironment suppresses NK cell function and allows cancer to evade detection. In this study, we investigated whether thyroid has effect on cells. We found that prostaglandin (PG) E2 produced by suppressed cytolytic activity of inhibiting expression natural cytotoxicity receptors NKp44 NKp30 death receptor necrosis factor-related apoptosis-inducing ligand. PGE2 cyclooxygenase-2 were highly expressed...
The doses of donor-derived natural killer (NK) cells that can be given safely after human leukocyte antigen (HLA)-haploidentical hematopoietic cell transplantation (HCT) remain to defined. Forty-one patients (ages 17 75 years) with hematologic malignancy underwent HLA-haploidentical HCT reduced-intensity conditioning containing busulfan, fludarabine, and antithymocyte globulin. Cell donors 7 62 growth factor-mobilized leukapheresis for 3 4 days. Cells collected on the first 2 days were used...
Thioredoxin-interacting protein (TXNIP) has emerged as a key player in cancer and diabetes since it targets thioredoxin (TRX)-mediated redox regulation glucose transporter (GLUT)-mediated metabolism. TXNIP consists of two arrestin (ARR, N-ARR C-ARR) domains at its amino-terminus PPxY (PY) motifs di-leucine (LL) motif for endocytosis carboxyl-terminus. Here, we report that shuffles between TRX GLUTs to regulate homeostasis intracellular oxidative stress While functions gatekeeper by default,...
Abstract Stimulation of human lung fibroblast cells with TGF-β1 resulted in a transient burst reactive oxygen species maximal increase at 5 min after treatment. This was inhibited by the antioxidant, N-acetyl-l-cysteine (NAC). treatment stimulated IL-6 gene expression and protein synthesis cells. Antioxidants including NAC, glutathione, catalase reduced TGF-β1-induced expression, direct H2O2 induced dose-dependent manner. NAC also AP-1 binding activity, which is involved expression. It has...
Vitamin D3 up-regulated protein 1 (VDUP1) is a stress-response gene that by 1,25(OH)2D3 in many cells. It has been reported VDUP1 expression reduced tumor cells and the enforced of inhibits cell proliferation arresting cycle progression. Here, we found VDUP1-/- fibroblast proliferated more rapidly compared with wild-type p27(kip1), cyclin-dependent kinase inhibitor. JAB1 known to interact p27(kip1) decrease stability p27(kip1). interacted restored JAB1-induced suppression stability. In this...
Activation of hepatic stellate cells (HSC) has been identified as a critical step in fibrogenesis and is regulated by several factors including cytokines oxidative stress. However, the molecular mechanism for HSC inactivation not well understood. We investigated an<i>N</i>-acetyl-l-cysteine (NAC)-mediated signaling pathway involved inactivation. NAC, which acting through its reducing activity, induced cell arrest at G<sub>1</sub> via mitogen-activated protein kinase (MAPK) (MEK)/MAPK...
Abstract NK cells are a key component of innate immune systems, and their activity is regulated by cytokines hormones. Adiponectin, which secreted from white adipose tissues, plays important roles in various diseases, including hypertension, cardiovascular inflammatory disorders, cancer. In this study the effect adiponectin on cell was investigated. Adiponectin found to suppress IL-2-enhanced cytotoxic without affecting basal cytotoxicity inhibit IL-2-induced NF-κB activation via...
Abstract Vitamin D3 upregulated protein 1 (VDUP1) is a candidate tumor suppressor, the expression of which dramatically reduced in various tissues. In this study, we found that VDUP1 suppressed during human hepatic carcinogenesis, and mice lacking are much more susceptible to diethylnitrosamine-induced hepatocarcinogenesis compared with wild type mice. VDUP1-deficient tumors proliferated significantly than had corresponding changes key cell cycle regulatory proteins. addition,...
BackgroundPerforin-1 (Prf1) is the predominant cytolytic protein secreted by natural killer (NK) cells. For a rapid immune response, resting NK cells contain high Prf1 mRNA concentrations while exhibiting minimal cytotoxicity caused blockage of synthesis, implying that an unknown posttranscriptional regulatory mechanism exists.ObjectiveWe sought to determine whether microRNA-150 (miR-150) posttranscriptionally regulates translation in both mouse and human at rest various time points after...