A5102007547- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- NF-κB Signaling Pathways
- Vibrio bacteria research studies
- Cytokine Signaling Pathways and Interactions
- Bone Metabolism and Diseases
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- Immune Cell Function and Interaction
- TGF-β signaling in diseases
- Inflammatory mediators and NSAID effects
- Nitric Oxide and Endothelin Effects
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Hepatitis B Virus Studies
- RNA modifications and cancer
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Redox biology and oxidative stress
- Fungal Biology and Applications
- Retinoids in leukemia and cellular processes
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Vitamin D Research Studies
Seoul National University Dental Hospital
2009-2022
Pohang University of Science and Technology
2008-2022
Seoul Veterans Hospital
2022
Life University
2017
University of Massachusetts Chan Medical School
2017
Soonchunhyang University
2016
Seoul National University
2008-2014
International Vaccine Institute
2002-2011
Korea Research Institute of Bioscience and Biotechnology
2000-2003
Korea Advanced Institute of Science and Technology
1998-2000
Abstract As a result of identifying the regulatory proteins thioredoxin (TRX), murine homologue for human vitamin D3 up-regulated protein 1 (VDUP1) was identified from yeast two-hybrid screen. Cotransfection into 293 cells and precipitation assays confirmed that mouse VDUP1 (mVDUP1) bound to TRX, but it failed bind Cys32 Cys35 mutant suggesting redox-active site is critical binding. mVDUP1 ubiquitously expressed in various tissues located cytoplasm. Biochemical analysis showed inhibited...
Complement-C1q TNF-related protein 1 (CTRP1), a member of the CTRP superfamily, is expressed at high levels in adipose tissues obese Zucker diabetic fatty (fa/fa) rats, and CTRP1 expression induced by proinflammatory cytokines, including TNF-alpha IL-1beta. In present study, we investigated stimulation aldosterone production CTRP1, since it was observed that specifically zona glomerulosa adrenal cortex, where produced. Increased cells human cortical cell line H295R dose-dependent. Expression...
Abstract Stimulation of human lung fibroblast cells with TGF-β1 resulted in a transient burst reactive oxygen species maximal increase at 5 min after treatment. This was inhibited by the antioxidant, N-acetyl-l-cysteine (NAC). treatment stimulated IL-6 gene expression and protein synthesis cells. Antioxidants including NAC, glutathione, catalase reduced TGF-β1-induced expression, direct H2O2 induced dose-dependent manner. NAC also AP-1 binding activity, which is involved expression. It has...
2-Arachidonyl-glycerol (2-Ara-Gl) recently was identified as a putative endogenous ligand for cannabinoid receptor types CB1 and CB2 by competitive binding. More recent immune function assays demonstrated that 2-Ara-Gl possessed immunomodulatory activity. Because several plant-derived cannabinoids inhibit interleukin-2 (IL-2) expression, investigated its ability to modulate this cytokine. The direct addition of mouse splenocyte cultures suppressed phorbol-12-myristate-13-acetate plus...
Silymarin, a polyphenolic flavonoid antioxidant, is known to have anti-inflammatory, hepatoprotective, and anticarcinogenic effects. In the present study, we report inhibitory effect of silymarin on nitric oxide production inducible nitric-oxide synthase (iNOS) gene expression in macrophages. vivo administration attenuated by peritoneal macrophages lipopolysaccharide (LPS)-treated mice. Silymarin also dose dependently suppressed LPS-induced isolated mouse RAW 264.7, murine macrophage-like...
Abstract Background Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and prevent infection caused by SARS-CoV. Polyethylenimine 25K (PEI) a cationic polymer which effectively delivers plasmid DNA. Results In present study, immune responses BALB/c immunized via intranasal (i.n.) route SARS DNA vaccine (pci-S) PEI/pci-S complex form have been examined. The size nanoparticles appeared be...
Abstract Constitutive IL-18 expression is detected from many different cells, including macrophages, keratinocytes, and osteoblasts. It has been known that gene regulated by two promoters (p1 promoter p2 promoter). When RAW 264.7 macrophages were treated with IFN-γ, was increased in a dose- time-dependent manner. IFN-γ activated the inducible 1, but not constitutive 2. Mutagenesis studies indicated an IFN consensus sequence-binding protein (ICSBP) binding site between −39 −22 critical for...
A conserved protein kinase VRK-1 increases life span by activating a key energy sensor AMPK in the roundworm C. elegans .
Abstract The decrease in NO production was found to correlate well with a inducible nitric oxide synthase (iNOS) mRNA expression as demonstrated by Northern blot analysis and quantitative RT‐PCR. Since the promoter iNOS gene contains binding motifs for NF‐κB/Rel, NF‐IL6, Oct which appear be important LPS‐mediated induction, effects of DEX on activation these transcription factors were examined. Treatment RAW 264.7 cells induced dose‐related inhibition NF‐κB/Rel chloramphenicol...
Vaccinia-related kinase 1 (VRK1) and VRK3 are members of the VRK family serine/threonine kinases principally localized in nucleus.Despite crucial roles VRK1/ physiology disease, molecular functional interactions VRK1/VRK3 poorly understood.Here, we identified over 200 unreported VRK1/VRK3-interacting candidate proteins by affinity purification LC-MS/MS.The networks VRK1 interactomes were found to be associated with important biological processes such as cell cycle, DNA repair, chromatin...
Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosides—the major active components of ginseng—exhibit immunomodulatory effects. However, the mechanism and function underlying effects are not fully elucidated, especially monocytes dendritic cells (DCs). We investigated effect ginsenosides from on CD14+ purified adult peripheral blood mononuclear (PBMCs) their...
Deinococcus radiodurans is an extremely resistant bacterium against extracellular stress owing to on its unique physiological functions and the structure of cellular constituents. Interestingly, it has been reported that pattern alteration in proportion skin negatively correlated with inflammatory diseases, whereas Staphylococcus aureus was increased patients chronic diseases. However, biological mechanisms deinococcal interactions other commensal bacteria have not studied. In this study, we...
Abstract Hericium erinaceum , a medicinal mushroom, has long been used as therapeutic due to its immuno‐regulating potentials eliciting anticarcinogenic and antimicrobial efficacies. Since maturation of dendritic cells (DC) is an important process in the initiation regulation immune responses, ability water‐soluble components from H. (WEHE) regulate DC was investigated. Immature were prepared by differentiating human peripheral blood CD14‐positive with GM‐CSF IL‐4. stimulated WEHE at 2–20...
Childhood immunization rates are at an all-time high globally, and national data for China suggests close to universal coverage. Refugees from North Korea their children may have more limited health care access in due legal status. However, there is no on or barriers coverage this population.This study was conducted determine the correlates of immunizations (≥1 year) born Korean refugees Yanbien, China. Child obtained vaccination cards caregiver self-report 7 vaccines 1:3:3:3:1 series....