A5101761961- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Plant and Fungal Interactions Research
- Toxin Mechanisms and Immunotoxins
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Animal Genetics and Reproduction
- Genetically Modified Organisms Research
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Animal Virus Infections Studies
- Insect symbiosis and bacterial influences
- Microbial infections and disease research
- Mycotoxins in Agriculture and Food
- Phytase and its Applications
- Plant Pathogens and Fungal Diseases
- Forest Insect Ecology and Management
- Plant nutrient uptake and metabolism
- Glycosylation and Glycoproteins Research
Jeonbuk National University
2011-2024
Korea Basic Science Institute
2010-2014
Chonbuk National University Hospital
2006-2011
Hue University
2010
Plant Industry
2008
Bank of Korea
2002
Abstract Background The wild grass species Brachypodium distachyon (Brachypodium hereafter) is emerging as a new model system for crop genomics research and biofuel biology. A draft nuclear genome sequence expected to be publicly available in the near future; an explosion of gene expression studies will undoubtedly follow. Therefore, stable reference genes are necessary normalize data. Results systematic exploration suitable presented here. Nine candidates were chosen, their sequences...
Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated culture medium dropped quickly from its maximum 150 microg/L at 5 d after incubation. To overcome this problem, we sought an expression system which heterologous gene could be induced high levels. We selected a rice amylase promoter Ramy3D is to express recombinant protein by sucrose starvation. This...
Summary We examined the biological function of cpmk 1, which encodes a MAPK Cryphonectria parasitica , and its regulation by mycovirus. Sequence comparisons revealed that 1 had highest homology with osm hog 1‐homologue from Magnaporthe grisea . A growth defect was observed in 1‐null mutant under hyperosmotic conditions, indicating functionally belongs to subfamily. Immunoblot analyses indicated CpMK1 pathway affected specifically conditions hypovirus CHV1‐EP713. Moreover, virus‐infected...
DNA extraction methods for PCR-quality from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of pellet ethanol, washing drying pellet, etc. The need a rapid simple procedure is urgent, especially when hundreds samples to analyzed. Here, we describe efficient method isolating high-quality genomic PCR amplification enzyme digestion calluses, various wild-type transgenic plants.We developed new reliable...
Abstract Background Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and prevent infection caused by SARS-CoV. Polyethylenimine 25K (PEI) a cationic polymer which effectively delivers plasmid DNA. Results In present study, immune responses BALB/c immunized via intranasal (i.n.) route SARS DNA vaccine (pci-S) PEI/pci-S complex form have been examined. The size nanoparticles appeared be...
Summary The rice α‐amylase 3D promoter system, which is activated under sucrose‐starved conditions, has emerged as a useful system for producing recombinant proteins. However, using the production therapeutic proteins requires modifications of N ‐glycosylation pattern because potential immunogenicity plant‐specific sugar residues. In this study, glyco‐engineered were generated host glycoproteins, RNA interference (RNAi) technology to down‐regulate endogenous α‐ 1,3‐fucosyltransferase (...
It has been suggested that plant cell culture is the most suitable system for producing small-to-medium quantities of specialized, expensive, and high-purity proteins. Here, we report a heterodimeric protein, human interleukin-12 (hIL-12), was expressed secreted into medium in biologically active form. A transgenic expressing hIL-12 constructed by sexual crossing plants each subunit protein. From piece plant, callus induced suspension established. The biological activity amount were analyzed...
In order to enhance vaccine uptake by the immune cells in vivo, molecular engineering approach was employed construct a polymeric immunoglobulin G scaffold (PIGS) that incorporates multiple copies of an antigen and targets Fc gamma receptors on antigen-presenting cells. These self-adjuvanting immunogens were tested context dengue infection, for which there is currently no globally licensed yet. Thus, consensus domain III sequence (cEDIII) glycoprotein E incorporated into PIGS expressed both...
Dengue is a major global disease requiring improved treatment and prevention strategies. The recently licensed Sanofi Pasteur Dengvaxia vaccine does not protect children under the age of nine, additional strategies are thus needed to halt this expanding epidemic. Here, we employed molecular engineering approach plant expression produce humanized highly immunogenic poly-immunoglobulin G scaffold (PIGS) fused consensus dengue envelope protein III domain (cEDIII). immunogenicity IgG Fc...