A5030232851- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Enzyme Structure and Function
- Hippo pathway signaling and YAP/TAZ
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- SARS-CoV-2 detection and testing
- Immune responses and vaccinations
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Microbial Natural Products and Biosynthesis
- Biosensors and Analytical Detection
- Redox biology and oxidative stress
- Cancer-related Molecular Pathways
- Genetics and Neurodevelopmental Disorders
- Angiogenesis and VEGF in Cancer
- interferon and immune responses
- Heat shock proteins research
- Clostridium difficile and Clostridium perfringens research
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
Korea Research Institute of Bioscience and Biotechnology
2019-2025
Korea Basic Science Institute
2012-2023
Pusan National University Yangsan Hospital
2023
Chonnam National University
2017-2022
Korea University
2012-2014
Korea University of Science and Technology
2010
The redox-dependent inhibition of thioredoxin (TRX) by thioredoxin-interacting protein (TXNIP) plays a pivotal role in various cancers and metabolic syndromes. However, the molecular mechanism this regulation is largely unknown. Here, we present crystal structure TRX–TXNIP complex demonstrate that TRX TXNIP mediated an intermolecular disulphide interaction resulting from novel bond-switching mechanism. Upon binding to TRX, undergoes structural rearrangement involves switching head-to-tail...
In eukaryotic cells, apoptosis and cell cycle arrest by the Ras --> RASSF MST pathway are controlled interaction of SARAH (for Salvador/Rassf/Hippo) domains in C-terminal part tumor suppressor proteins. The Mst1 domain interacts with its homologous Rassf1 Rassf5 (also known as Nore1) forming a heterodimer that mediates process. Here, we describe homodimeric structure human heterotypic Salvador (Sav) domain. forms homodimer containing two helices per monomer. An antiparallel arrangement long...
NMR structural studies of membrane proteins (MP) are hampered by complications in MP expression, technical difficulties associated with the slow process spectral peak assignment, and limited distance information obtainable for transmembrane (TM) helices. To overcome inherent challenges determination structures, we have developed a rapid cost-efficient strategy that combines cell-free (CF) protein synthesis, optimized combinatorial dual-isotope labeling nearly instant resonance fast...
A previously unknown SARS-CoV-2 vaccine protecting nonhuman primates from challenge was developed using RBD-P2 and N.
Abstract The E6 and E7 proteins of specific subtypes human papillomavirus (HPV), including HPV 16 18, are highly associated with cervical cancer as they modulate cell cycle regulation. aim this study was to investigate the potential antitumor effects a messenger RNA‐HPV therapeutic vaccine (mHTV) containing nononcogenic proteins. To achieve this, C57BL/6j mice were injected via both intramuscular subcutaneous routes, resulting evaluated. mHTV immunization markedly induced robust T...
Despite recent progress in research on the Hippo signalling pathway, structural information available this area is extremely limited. Intriguingly, homodimeric and heterodimeric interactions of mammalian sterile 20-like (MST) kinases through so-called `SARAH' (SAV/RASSF/HPO) domains play a critical role cellular homeostasis, dictating fate cell regarding proliferation or apoptosis. To understand mechanism heterodimerization SARAH domains, three-dimensional structures an MST1–RASSF5...
Abstract Using a reliable primate model is critical for developing therapeutic advances to treat humans infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we exposed macaques high titers of SARS-CoV-2 via combined transmission routes. We observed interstitial pneumonia endotheliitis in the lungs all macaques. All had significant loss total lymphocytes during infection, which were restored over time. These data show that causes coronavirus disease 2019...
Human gastric adenocarcinoma (AGS) is one of the most common types malignant tumor and third-leading cause tumor-associated mortality worldwide. Withaferin A (WA), a steroidal lactone derived from Withania somnifera, exhibits antitumor activity in variety cancer models. However, to best our knowledge, direct effect WA on AGS cells has not previously been determined. The present study investigated effects proliferation metastatic cells. exerted dose-dependent cytotoxic was associated with...
Hyeon-Gu Yeo, Jung Joo Hong, Youngjeon Lee, Kyung Sik Yi, Chang-Yeop Jeon, Junghyung Park, Jinyoung Won, Jincheol Seo, Yu-Jin Ahn, Keonwoo Kim, Seung Ho Baek, Eun-Ha Hwang, Green Yeung Bae Jin, Kang-Jin Jeong, Bon-Sang Koo, Philyong Kang, Seob Lim, Sun-Uk Jae-Won Huh, Young-Hyun Yeonghoon Son, Ji-Su Chi-Hoon Choi, Sang-Hoon Cha and Sang-Rae Lee. Exp Neurobiol 2019;28:458-73. https://doi.org/10.5607/en.2019.28.4.458
In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto origin of chromosome. The C-terminal domain mouse (mCdt1C), most conserved region in Cdt1, is essential for licensing and directly interacts with complex. We have determined structures mCdt1CS (mCdt1C_small; residues 452 to 557) mCdt1CL (mCdt1C_large; 420 using X-ray crystallography solution NMR spectroscopy, respectively. While N-terminal 31 form flexible loop short helix near middle, rest...
Much attention has been focused on the design and synthesis of potent, cationic antimicrobial peptides (AMPs) that possess both anti-inflammatory activities. However, their development into therapeutic agents limited mainly due to large size (12 50 residues in length) poor protease stability.In an attempt overcome issues described above, a set ultra-short, His-derived (HDAMPs) developed for first time. Through systematic tuning pendant hydrophobic alkyl tails at N(π)- N(τ)-positions His,...
Background Polo-like kinase-1 (Plk1) plays a crucial role in cell proliferation and the inhibition of Plk1 has been considered as potential target for specific inhibitory drugs anti-cancer therapy. Several research groups have identified peptide-based inhibitors that polo-box domain (PBD) bind to protein with high affinity vitro assays. However, inadequate proteolytic resistance permeability peptides hinder development these into novel therapeutic compounds. Methodology/Principal Findings In...
Abstract DJ‐1, a protein encoded by PARK7 plays protective role against neurodegeneration. Since its glyoxalase III activity catalyzing methylglyoxal (MG) to lactate was discovered, DJ‐1 has been re‐established as deglycase decomposing the MG‐intermediates with amino acids and nucleotides (hemithioacetal hemiaminal) rather than MG itself, but it is still debatable. Here, we have clarified that human directly recognizes MG, not MG‐intermediates, monitoring detailed catalytic processes...
Abstract Considering the emergence of bacterial resistance and low proteolytic stability antimicrobial peptides (AMPs), herein we developed a series ultra-short triazine based amphipathic polymers (TZP) that are connected with ethylene diamine linkers instead protease sensitive amide bond. The most potent oligomers, TZP3 TZP5 not only displayed antibacterial action on various drug-resistant pathogens but also exhibited strong synergic activity in combination chloramphenicol against...
Abstract Recently emerging severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) Omicron variants are generally less pathogenic than previous strains. However, elucidating the molecular basis for pulmonary immune response alterations is challenging owing to virus's heterogeneous distribution within complex tissue structure. Here, we revealed spatial transcriptomic profiles of microstructures at SARS‐CoV‐2 infection site in nine cynomolgus macaques upon inoculation with Delta and...
Over the years, a great deal of effort has been focused on design and synthesis potent, linear peptide inhibitors targeting polo-like kinase 1 (Plk1), which is critically involved in multiple mitotic processes established as an adverse prognostic marker for tumor patients. Plk1 localizes to its intracellular anchoring sites via polo-box domain, inhibiting domain considered approach circumvent specificity problems associated with conserved adenosine triphosphate-binding pocket. The consists...
The two members of the cytoplasmic FMR1-interacting protein family, CYFIP1 and CYFIP2, are evolutionarily conserved multifunctional proteins whose defects associated with distinct types brain disorders. Even high sequence homology between several lines evidence indicate their different functions in brain; however, underlying mechanisms remain largely unknown. Here, we performed reciprocal immunoprecipitation experiments using CYFIP1-2 × Myc CYFIP2-3 Flag knock-in mice found that CYFIP2 not...
Characterizing the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at molecular level is necessary understand viral pathogenesis and identify clinically relevant biomarkers. However, in humans, pulmonary during disease onset remains poorly understood. Herein, we utilized a spatial transcriptome atlas microstructure-specific COVID-19 gene signatures phase of lung infection cynomolgus macaques. The innate immune virus-induced cell death was primarily active...