A5056640582- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Immune responses and vaccinations
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Nutrition and Physiology
- Animal Disease Management and Epidemiology
- Gut microbiota and health
- Virus-based gene therapy research
- Parkinson's Disease Mechanisms and Treatments
- SARS-CoV-2 detection and testing
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Acute Ischemic Stroke Management
- Aortic aneurysm repair treatments
- Alzheimer's disease research and treatments
- Parvovirus B19 Infection Studies
- Aquaculture disease management and microbiota
- Respiratory viral infections research
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Korea University of Science and Technology
2022-2025
Chungbuk National University
2011-2017
Asan Medical Center
2003
University of Ulsan
2003
Abstract Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting receptor binding domain (RBD) of viral spike protein via antibody library constructed from peripheral blood mononuclear cells a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including D614G variant without antibody-dependent enhancement effect. Complex crystal...
SIRT3 (sirtuin 3), a mitochondrial protein deacetylase, maintains respiratory function, but its role in the regulation of innate immune defense is largely unknown. Herein, we show that coordinates function and macroautophagy/autophagy activation to promote anti-mycobacterial responses through PPARA (peroxisome proliferator activated receptor alpha). deficiency enhanced inflammatory dysfunction, leading defective host pathological inflammation during mycobacterial infection. Antibody-mediated...
Vaccination constitutes one of the most important means preventing infectious disease in captive nonhuman primates (NHPs). protocols for NHPs vary, as they are mostly guided by institutional preference, infection pressure, local availability, and recommendations non-peer reviewed resources. Currently, no updated literature review about vaccination options NHP is available. Therefore, we provide a detailed overview published NHP. Our findings demonstrate that, while there often insufficient...
Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters cynomolgus and rhesus monkeys limited. In the present study, we established hematologic biochemical intervals healthy anesthetized with ketamine hydrochloride. A total of 142 (28 males 114 females) 42 (22 20 were selected analyzed order to examine hematological 16 parameters. The effects sex also investigated. Reference separately by species (cynomolgus rhesus) (male...
Bat species around the world have recently been recognized as major reservoirs of several zoonotic viruses, such severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East (MERS-CoV), Nipah virus and Hendra virus. In this study, consensus primer-based reverse transcriptase polymerase chain reactions (RT-PCRs) high-throughput sequencing were performed to investigate viruses in bat faecal samples collected at 11 natural habitat sites from July December 2015 Korea. Diverse...
X-box binding protein 1 (XBP1) mRNA processing plays a crucial role in the unfolded response (UPR), which is activated to endoplasmic reticulum (ER) stress. Upon accumulation of UPR-converted XBP1 splicing from an unspliced (u) (inactive) isoform spliced (s) (active) isoform, inositol-requiring enzyme α (IRE1α) removes 26-nucleotide intron uXBP1 mRNA. Recent studies have reported assessment ER stress by examining ratio sXBP1 (s/uXBP1 ratio) via densitometric analysis PCR bands relative...
A previously unknown SARS-CoV-2 vaccine protecting nonhuman primates from challenge was developed using RBD-P2 and N.
The unprecedented and rapid spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) has motivated the need for a rapidly producible scalable vaccine. Here, we developed synthetic soluble spike (S) DNA-based vaccine candidate, GX-19. In mice, immunization with GX-19 elicited not only S-specific systemic pulmonary antibody responses but also Th1-biased T cell in dose-dependent manner. GX-19-vaccinated nonhuman primates seroconverted exhibited detectable neutralizing response as...
Mitochondria continuously fuse and divide to maintain homeostasis. An impairment in the balance between fusion fission processes can trigger mitochondrial dysfunction. Accumulating evidence suggests that dysfunction is related neurodegenerative diseases such as Parkinson's disease (PD), with excessive dopaminergic neurons being one of pathological mechanisms PD. Here, we investigated substantia nigra a non-human primate model 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced We...
Abstract Using a reliable primate model is critical for developing therapeutic advances to treat humans infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we exposed macaques high titers of SARS-CoV-2 via combined transmission routes. We observed interstitial pneumonia endotheliitis in the lungs all macaques. All had significant loss total lymphocytes during infection, which were restored over time. These data show that causes coronavirus disease 2019...
Since the outbreak of coronavirus disease (COVID-19) in 2019, severe acute respiratory syndrome 2 (SARS-CoV-2) has evolved into diverse variants. Here, an early isolate SARS-CoV-2 was serially passaged multiple cell lines human origin triplicate, and selected mutations were compared to those found natural In spike protein, Q493R Q498R substitutions from viruses consistent with B.1.1.529 (Omicron) variant. Y144del H655Y also reported B.1.1.7 (Alpha), P.1 (Gamma), Several single nucleotide...
Several enteric viruses have increasingly received attention as potential causative agents of runting-stunting syndrome (RSS) in chickens. A molecular survey was performed to determine the presence a broad range viruses, namely chicken astrovirus (CAstV), avian nephritis virus (ANV), parvovirus (ChPV), infectious bronchitis (IBV), rotavirus (AvRV), reovirus (ARV), and fowl adenovirus (FAdV), intestinal samples derived from 34 commercial flocks that experienced enteritis outbreaks between...
The guidelines for applying individual adjustments to macaques according the severity of behavioral symptoms during 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment were provided reproduce stable chronic Parkinsonism in a recent study (Potts et al., 2014). But, since there are insufficient regarding objective criteria MPTP treatment, it is difficult develop MPTP-induced non-human primate (NHP) models with symptoms.The administration based on results automatic quantification...
Study of interactions between the nervous system and immunity offers insights into pathogenesis Parkinson's disease (PD) potential therapeutic strategies for neurodegenerative diseases. Studies on rodents have revealed regulatory mechanisms microglial activation T lymphocyte recruitment in PD. However, underlying chronic infiltration brain after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injection a non-human primate (NHP) model PD remain unknown. This study aimed to investigate...
Hyeon-Gu Yeo, Jung Joo Hong, Youngjeon Lee, Kyung Sik Yi, Chang-Yeop Jeon, Junghyung Park, Jinyoung Won, Jincheol Seo, Yu-Jin Ahn, Keonwoo Kim, Seung Ho Baek, Eun-Ha Hwang, Green Yeung Bae Jin, Kang-Jin Jeong, Bon-Sang Koo, Philyong Kang, Seob Lim, Sun-Uk Jae-Won Huh, Young-Hyun Yeonghoon Son, Ji-Su Chi-Hoon Choi, Sang-Hoon Cha and Sang-Rae Lee. Exp Neurobiol 2019;28:458-73. https://doi.org/10.5607/en.2019.28.4.458
Chicken parvovirus (ChPV) is one of the causative agents viral enteritis. Recently, genome ABU-P1 strain ChPV was fully sequenced and determined to have a distinct genomic composition compared with that vertebrate parvoviruses. However, no comparative sequence analysis coding regions ChPVs possible because lack other information. In this study, we obtained nucleotide sequences all three by polymerase chain reaction using 13 primer sets, deduced amino acid from sequences. The non-structural...
Infectious coryza (IC) is an infectious disease caused by Avibacterium (Av.) paragallinarum.IC known to cause economic losses in the poultry industry via decreased egg production layers.Between 2012 and 2013, Av. paragallinarum was isolated from seven chicken farms Chungbuk National University.We identified paragallinarum, causative pathogen of IC polymerase chain reaction (PCR) serovar serotype A, multiplex PCR.Antibiotic sensitivity tests indicated that a few field-isolated strains showed...
The microbial community is known to have a key role during the rearing period of broilers.In this study, gut composition and diversity were examined evaluate relationships between these factors broiler growth performance.By applying 454-pyrosequencing V1-V3 regions bacterial 16S rRNA genes, six fecal samples from four-and 28-day-old chickens three farms 24 intestinal broilers with heavy light body weights analyzed.Microbial assessment revealed Firmicutes be most prevalent phylum at farm A,...
Infectious bronchitis virus (IBV) replicates primarily in the respiratory tract and grows various organs chickens, with or without pathological effects. The diversity of this has been verified by sequence analysis S1 glycoprotein gene, but method must be supplemented further for characterization agent. To increase our understanding pathogenesis disease caused virus, we investigated response chickens to 2 IBV different genotypes, KIIa ChVI. clinical signs induced viruses were observed. In...
Outbreaks of highly pathogenic avian influenza (HPAI) virus, subtype H5N8, were observed in two different flocks local broiler breeder farms and a commercial layer farm South Korea. Clinically, the cases characterized by gradual increase mortality, slow transmission, unrecognizable clinical signs HPAI. Gross observations both included hemorrhagic or necrotic lesions internal organs, such as serosal mucosal membranes, spleen, pancreas. Both exhibited similar histopathologic lesions, including...
A molecular study of intestinal samples from 21 broiler flocks with a history enteritis revealed that 23.8% and 14.3% were positive for chicken astrovirus (CAstV) avian rotavirus (ARV), respectively.CAstV group ARV simultaneously detected in only one flock.Birds this developed the significant lesions characterized by frothy contents, paleness, thin walls.In report we present an unusual case runting stunting syndrome (RSS) high mortality growth retardation chickens.We also make first...
Eggs exhibiting eggshell apex abnormalities (EAA) were evaluated for changes in shell characteristics such as strength, thickness, and ultrastructure. Mycoplasma synoviae (MS) infection was confirmed by serological assay along with isolation of MS from the trachea oviduct. Changes quality shown to be statistically significant (p < 0.01). We also identified ultrastructural mammillary knob layer Scanning Electron Microscopy. While eggs may seem structurally sound, evaluation showed that...
Abstract Recently emerging severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) Omicron variants are generally less pathogenic than previous strains. However, elucidating the molecular basis for pulmonary immune response alterations is challenging owing to virus's heterogeneous distribution within complex tissue structure. Here, we revealed spatial transcriptomic profiles of microstructures at SARS‐CoV‐2 infection site in nine cynomolgus macaques upon inoculation with Delta and...
A total of 600 wild birds were analyzed for the causes mortality in Republic Korea (ROK) from 2011 to 2013. Avian poxvirus (APV) infections identified as primary cause 39% (29/74) Oriental Turtle Doves (Streptopelia orientalis). At necropsy, all 29 S. orientalis birds, which, 76% (22/29) juveniles, had severe diphtheritic lesions their oral and nasal cavities on eyelids, which APV that resulted mortality. We detected infection by chorioallantoic membrane inoculation molecular study partial...