A5101515800- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Microtubule and mitosis dynamics
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- MicroRNA in disease regulation
- Virus-based gene therapy research
- DNA Repair Mechanisms
- Mycotoxins in Agriculture and Food
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Prenatal Screening and Diagnostics
- RNA Research and Splicing
- Reproductive System and Pregnancy
- Autophagy in Disease and Therapy
- Ovarian function and disorders
- Genetics, Aging, and Longevity in Model Organisms
- Viral Infectious Diseases and Gene Expression in Insects
- Xenotransplantation and immune response
- Wnt/β-catenin signaling in development and cancer
Wuyi University
2021-2024
Jilin University
2016-2024
Haier Group (China)
2024
Chungbuk National University
2014-2023
Wuyi University
2020-2022
Jilin Medical University
2020
Seoul National University Bundang Hospital
2018
Seoul National University
2018
Nanjing Agricultural University
2014
Catholic University of Daegu
2012
Mammalian oocyte meiotic maturation involves polarization and a unique asymmetric division, but until now, the underlying mechanisms have been poorly understood. Arp2/3 complex has shown to regulate actin nucleation is widely involved in diverse range of processes such as cell locomotion, phagocytosis establishment polarity. Whether participates division unknown. The present study investigated expression functions during mouse maturation. Immunofluorescent staining showed that was restricted...
// Teng Wang 1 , Jun Han Xing Duan Bo Xiong Xiang-Shun Cui 2 Nam-Hyung Kim Hong-Lin Liu Shao-Chen Sun College of Animal Science and Technology, Nanjing Agricultural University, 210095, China Department Sciences, Chungbuk National Cheongju 361-763, Korea Correspondence to: Sun, e-mail: sunsc@njau.edu.cn Keywords: BPA, cytoskeleton, autophagy/apoptosis, oxidative stress, epigenetics Received: January 20, 2016 Accepted: March 28, Published: April 11, 2016 ABSTRACT...
T-2 toxin is a main type A trichothecene mycotoxin which the most toxic trichothecence. has posed various effects on human and animals in vigorous cell proliferation tissues like lymphoid, hematopoietic gastrointestinal tissues, while HT-2 major metabolite deacetylated by toxin. In this study, we focused of porcine oocyte maturation. We treated with vitro, first found that treatment inhibited polar body extrusion cumulus expansion. observed disrupted meiotic spindle morphology after...
If no fertilization occurs at an appropriate time after ovulation, oocyte quality deteriorates rapidly as a process called postovulatory aging. Because the aging of oocytes has detrimental effects on embryo development and offspring, many efforts have been made to prevent Here we showed that quercetin prevented decline in during oocytes. Quercetin treatment reduced aging-induced morphological changes reactive oxygen species accumulation. Moreover, attenuated aging-associated abnormalities...
In 2011, DEHP (plasticizer) was reported to illegally be added in food and beverage products Taiwan, which caused great concerns about safety worldwide. DHEP has multiple toxic effects human animals such as endocrine disruption, cardiotoxicity, reproductive function, development defects. However, the of on mammalian oocyte quality are still unclear. Since MEHP is active metabolite vivo, this study we used porcine model explore maturation also studied melatonin administration exposure-induced...
Microtubule and microfilament organization in porcine oocytes during maturation vivo vitro was imaged by immunocytochemistry laser scanning confocal microscopy. At the germinal vesicle stage, microtubules were not detected oocyte. After breakdown, a small microtubule aster observed near condensed chromatin. During prometaphase asters found association with each chromatin mass. The then elongated encompassed at metaphase-I stage. anaphase-I telophase-I meiotic spindle. Microtubules only...
The objective of this study was to examine the changes in microtubule and microfilament assembly aged porcine oocytes determine their developmental pattern after parthenogenetic activation. Porcine were cultured Whitten's medium containing 10% follicular fluid with hormonal supplements (eCG hCG) for 22 hr 40 additional culture without supplements. At 40, 50, 60 culture, fixed immunocytochemistry or activated by electrical pulse. In metaphase II stage oocytes, microtubules detected only...
This study was carried out to optimize conditions for using real time RT-PCR as an efficient and precise quantitative method estimating the transcript levels of genes expressed in samples containing miniscule amounts RNA, such single mammalian oocytes embryos. First, mouse eggs blastocysts, we tested three kinds RNA isolation or collection methods: TRIZOL reagent, oligo-dT conjugated beads, freeze/thaw cycles with reverse transcription buffer. There were no significant differences among...
Mammalian oocyte maturation is distinguished by asymmetric division that regulated primarily cytoskeleton, including microtubules and microfilaments. Small Rho GTPase RhoA a key regulator of cytoskeletal organization which regulates cell polarity, migration, division. In this study, we investigated the roles in mammalian meiosis early embryo cleavage. (1) Disrupting activity or knock down expression caused failure polar body emission. This may have been due to decreased actin assembly...
ATP is critical for oocyte maturation, fertilization, and subsequent embryo development. Both mitochondrial membrane potential copy number expand during maturation. In order to differentiate the roles of metabolic activity mtDNA we used two inhibitors, FCCP (carbonyl cyanide p-(tri-fluromethoxy)phenyl-hydrazone) ddC (2'3-dideoxycytidine), deplete (Δφm) number, respectively. (2000 nM) reduced production by affecting Δφm, decreased mRNA expression Bmp15 (bone morphogenetic protein 15),...
During oocyte meiosis, a spindle forms in the central cytoplasm and migrates to cortex. Subsequently, extrudes small body highly polarized egg; this process is regulated primarily by actin. ROCK Rho-GTPase effector that involved various cellular functions, such as stress fiber formation, cell migration, tumor invasion, motility. In study, we investigated possible roles for mouse meiosis. was localized around spindles after germinal vesicle breakdown colocalized with cytoplasmic actin...
Melatonin has antioxidant and scavenger effects in the cellular system. This research investigated protective underlying mechanisms of melatonin action porcine somatic cell nuclear transfer (SCNT) embryos. The results suggested that developmental competence SCNT embryos was considerably enhanced after treatment. In addition, attenuated increase reactive oxygen species levels induced by oxidative stress, decrease glutathione levels, mitochondrial dysfunction. Importantly, inhibited...
Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be endocrine disruptor and adverse effect on embryonic development of mammals. However, mechanism action limited. In this study, we investigated role porcine development. First, parthenotes were treated with different concentrations BPA. We found that blastocyst formation was impaired arrested at 4-cell stage after treatment 100 μm Second, ROS increased following addition BPA,...
Chrysoeriol (CHE) is a flavonoid substance that exists in many plants. It has various physiological and pharmacological effects, including anti-inflammatory, antioxidant, anti-tumor, protective activity, especially for the cardiovascular system liver. Among common livestock embryos, porcine embryos are often considered high-quality objects studying antioxidant mechanisms of oocytes. Because contain high levels lipids, they more vulnerable to external stimuli, which affect development. Our...