A5100628467- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Sperm and Testicular Function
- Renal and related cancers
- RNA modifications and cancer
- Prenatal Screening and Diagnostics
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Birth, Development, and Health
- Ovarian function and disorders
- Plant Molecular Biology Research
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- Lipid metabolism and biosynthesis
- Ubiquitin and proteasome pathways
Third Affiliated Hospital of Southern Medical University
2024-2025
Hainan Medical University
2025
Henan Agricultural University
2024-2025
Hainan General Hospital
2025
Institute of Zoology
2015-2024
Chinese Academy of Sciences
2015-2024
University of Chinese Academy of Sciences
2016-2024
State Key Laboratory of Stem Cell and Reproductive Biology
2020-2023
Ningxia Medical University
2023
Binzhou Medical University
2012-2023
In animals, mtDNA is always transmitted through the female and this termed “maternal inheritance.” Recently, autophagy was reported to be involved in maternal inheritance by elimination of paternal mitochondria Caenorhabditis elegans ; moreover, immunofluorescence, P62 LC3 proteins were also found colocalize sperm after fertilization mice. Thus, it has been speculated that may an evolutionary conserved mechanism for mitochondrial elimination. However, using two transgenic mouse strains, one...
Mammalian oocyte meiotic maturation involves polarization and a unique asymmetric division, but until now, the underlying mechanisms have been poorly understood. Arp2/3 complex has shown to regulate actin nucleation is widely involved in diverse range of processes such as cell locomotion, phagocytosis establishment polarity. Whether participates division unknown. The present study investigated expression functions during mouse maturation. Immunofluorescent staining showed that was restricted...
Abstract Proper follicle development is very important for the production of mature oocytes, which essential maintenance female fertility. This complex biological process requires precise gene regulation. The most abundant modification mRNA, N 6 -methyladenosine (m A), involved in many RNA metabolism processes, including splicing, translation, stability, and degradation. Here, we report that m A plays roles during oocyte development. Oocyte-specific inactivation key methyltransferase Mettl3...
Abstract Sperm‐induced Ca 2+ rise is critical for driving oocyte activation and subsequent embryonic development, but little known about how lasting oscillations are regulated. Here it shown that NLRP14, a maternal effect factor, essential keeping early development. Few embryos lacking NLRP14 can develop beyond the 2‐cell stage. The impaired developmental potential of Nlrp14 ‐deficient oocytes mainly caused by disrupted cytoplasmic function calcium homeostasis due to altered mitochondrial...
In mitosis, the spindle assembly checkpoint (SAC) prevents anaphase onset until all chromosomes have been attached to microtubules and aligned correctly at equatorial metaphase plate. The major proteins in mitosis consist of mitotic arrest-deficient (Mad)1-3, budding uninhibited by benzimidazole (Bub)1, Bub3, monopolar 1(Mps1). During meiosis, for formation a haploid gamete, two consecutive rounds chromosome segregation occur with only one round DNA replication. To pull homologous opposite...
ABSTRACT N 6‐methyladenosine (m 6 A) is the most prevalent and reversible internal modification of mammalian messenger noncoding RNAs mediated by specific m A writer, reader, eraser proteins. As an methyltransferase‐like 3‐methyltransferase–like 14 (METTL14)–Wilms tumor 1–associated protein complex dynamically regulates plays important roles in diverse biologic processes. However, our knowledge about complete functions this RNA methyltransferase complex, contributions each component to...
Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs delivers some to oocyte at fertilization. However, functions of carried by fertilization in embryonic development remains a mystery. In this study, spermatozoa were treated with lysolecithin, pronase RNases (RNase A RNase H) remove sperm-carried RNAs, then injected into normal oocyte. The results showed after treatment, content was decreased about 90%. blastocyst formation rate live birth...
During oocyte meiosis, a spindle forms in the central cytoplasm and migrates to cortex. Subsequently, extrudes small body highly polarized egg; this process is regulated primarily by actin. ROCK Rho-GTPase effector that involved various cellular functions, such as stress fiber formation, cell migration, tumor invasion, motility. In study, we investigated possible roles for mouse meiosis. was localized around spindles after germinal vesicle breakdown colocalized with cytoplasmic actin...
Elder women suffer from low or loss of fertility because decreasing oocyte quality as maternal aging. As energy resource, mitochondria play pivotal roles in development, determining quality. With advanced age, increased dysfunctions emerge mitochondria, which decrease and its developmental potential. Mitochondria supplement a possible strategy for improving egg has been debate due to ethnic problems. Heterogeneity is an intractable problem even transfer germinal vesicle, spindle, pronuclei...
Abstract Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that expressed in mouse oocytes from germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well time lapse live imaging after injection mRNA encoding fusion GFP identified was localized meiotic spindle, especially spindle poles at metaphase, while it concentrated midbody telophase oocytes. Knockdown by specific siRNA caused dissociation γ-tubulin poles,...
The quality of post-ovulatory oocytes decreases with aging. In this study, we aimed to investigate the effects N-acetyl-L-cysteine (NAC), a broadly used antioxidant, on oocyte in mouse aging vitro. NAC at 0.6mM concentration was added culture medium (M2), and analyzed 6h, 12h, 18h 24h culture. We found that frequency spindle defects decreased NAC-treated compared those without treatment. treatment significantly abnormal distribution cortical granules (CGs) during for 24h. Decreased...
Liver Kinase b1 (LKB1/STK11)is a tumor suppressor responsible for the Peutz-Jeghers syndrome, an autosomal-dominant, cancer-prone disorder in which patients develop neoplasms several organs, including oviduct, ovary, and cervix. Besides, C allele of SNP Lkb1 gene impedes likelihood ovulation polycystic ovary syndrome (PCOS) women treated with metformin, known LKB1-AMPK activator. It is very likely that LKB1 plays roles female fertility. To identify physiological functions mouse we...
Meiosis initiation is a crucial step for the production of haploid gametes, which occurs from anterior to posterior in fetal ovaries. The asynchrony transition mitosis meiosis results heterogeneity female germ cell populations, limits studies and progression at higher resolution level. To dissect process initiation, we investigated transcriptional profiles 19 363 single cells collected E12.5, E14.5, E16.5 mouse Clustering analysis identified seven groups defined dozens corresponding...
Abstract The formation of zygote is the beginning mammalian life, and dynamic epigenetic modifications are essential for normal development. H3K27 di-methylation (H3K27me2) tri-methylation (H3K27me3) marks facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, mechanism underlying establishment regulation asymmetry remains obscure. Here we show that maternal EZH2 required H3K27me3 mouse zygotes. combined...
Abstract Background Female infertility is a worldwide concern and the etiology of has not been thoroughly demonstrated. Although mouse good model system to perform functional studies, differences between human also need be considered. The objective this study elucidate different molecular mechanisms underlying oocyte maturation fertilization mouse. Results A comparative transcriptome analysis was performed identify differentially expressed genes associated biological processes oocytes. In...
Background Recently, a variant of ER-α, ER-α36 was identified and cloned. lacks intrinsic transcription activity mainly mediates non-genomic estrogen signaling. The purpose this study to investigate the function underlying mechanisms in growth regulation endometrial Ishikawa cancer cells. Methods cellular localization ER-α66 were determined by immunofluorescence control cells transfected with an empty expression vector, shRNA knockdown (Ishikawa/RNAiER36) (Ishikawa/RNAiER66) treated E2...
GM130, a cis-Golgi protein, plays key roles in various mitotic events, but its function mammalian oocyte meiosis remains unknown. In this study, we found that GM130 was localized to the spindle poles at both metaphase I and II stages associated with midbody telophase stage. The association of further confirmed by colocalization centrosome-associated proteins, MEK1/2. By nocodazole treatment, clarified localization consistently dependent on assembly. Then investigated possible specific...
Mammalian oocyte maturation is distinguished by highly asymmetric meiotic divisions during which a haploid female gamete produced and almost all the cytoplasm maintained in egg for embryo development. Actin-dependent meiosis I spindle positioning to cortex induces formation of polarized actin cap polarity, it determines resulting two polar bodies. Here we investigate functions Cdc42 oocyte-specific deletion through Cre-loxP conditional knockout technology. We find that causes infertility...