A5012207911- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- dental development and anomalies
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Craniofacial Disorders and Treatments
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- Cleft Lip and Palate Research
- Vascular Malformations and Hemangiomas
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Seed Germination and Physiology
- Enzyme Structure and Function
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
First Affiliated Hospital of Jiangxi Medical College
2025
Nanchang University
2025
Shanghai Jiao Tong University
2020-2024
Shanghai Ninth People's Hospital
2020-2024
National Clinical Research Center for Digestive Diseases
2023
Changhai Hospital
2023
Second Military Medical University
2023
Shanghai Mental Health Center
2022
University of California, Berkeley
2013-2020
Howard Hughes Medical Institute
2013-2020
Abstract Exploitation of custom-designed nucleases to induce DNA double-strand breaks (DSBs) at genomic locations choice has transformed our ability edit genomes, regardless their complexity. DSBs can trigger either error-prone repair pathways that random mutations the break sites or precise homology-directed generate specific insertions deletions guided by exogenously supplied DNA. Prior editing strategies using site-specific modify Caenorhabditis elegans genome achieved only heritable...
Increasing evidence points to nuclear compartmentalization as a contributing mechanism for gene regulation, yet mechanisms remain unclear. In this paper, we use autonomous targeting of bacterial artificial chromosome (BAC) transgenes reveal cis requirements peripheral targeting. Three regions (PTRs) within an HBB BAC bias competition between pericentric versus heterochromatin toward the periphery, which correlates with increased H3K9me3 across β-globin cluster and locus control region....
Mild mitochondrial stress experienced early in life can have beneficial effects on the span of organisms through epigenetic regulations. Here, we report that acetyl-coenzyme A (CoA) represents a critical signal to regulate aging chromatin remodeling and histone deacetylase complex (NuRD) Caenorhabditis elegans. Upon stress, impaired tricarboxylic acid cycle results decreased level citrate, which accounts for reduced production acetyl-CoA consequently induces nuclear accumulation NuRD...
Genomic regions preferentially associate with of similar transcriptional activity, partitioning genomes into active and inactive compartments within the nucleus. Here we explore mechanisms controlling genome compartment organization in Caenorhabditis elegans investigate roles for regulating gene expression. Distal arms C. chromosomes, which are enriched heterochromatic histone modifications H3K9me1/me2/me3, interact each other both cis trans, while interacting less frequently central...
During meiosis, chromosomes exhibit dramatic changes in morphology and intranuclear positioning. How these influence homolog pairing, alignment, recombination remain elusive. Using Hi-C, we systematically mapped 3D genome architecture throughout all meiotic prophase substages during mouse spermatogenesis. Our data uncover two major chromosome organizational features varying along the axis early prophase, when alignment occurs. First, transcriptionally active inactive genomic regions form...
Special AT-rich sequence binding protein 1 (SATB1) has long been proposed to act as a global chromatin loop organizer in T cells. However, the exact functions of SATB1 spatial genome organization remain elusive. Here we show that depletion human and murine cells leads transcriptional dysregulation for genes involved cell activation, well alterations 3D architecture at multiple levels, including compartments, topologically associating domains, loops. Importantly, extensively colocalizes with...
Abstract Telomeres, TTAGGGn DNA repeat sequences located at the ends of eukaryotic chromosomes, play a pivotal role in aging and are targets damage response. Although we others have demonstrated presence short telomeres genetic cardiomyopathic heart failure cardiomyocytes, little is known about telomere lengths cardiomyocyte. Here, demonstrate that patient shortened compared to healthy controls. We generated isogenic human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) with...
Abstract Chromosomes pair and synapse with their homologous partners to segregate correctly at the first meiotic division. Association of telomeres LINC (Linker Nucleoskeleton Cytoskeleton) complex composed SUN1 KASH5 enables telomere-led chromosome movements telomere bouquet formation, facilitating precise pairwise alignment homologs. Here, we identify a direct interaction between Speedy A (SPDYA) determine crystal structure human SUN1-SPDYA-CDK2 ternary complex. Analysis meiosis prophase I...
Abstract Chemicals or drugs can accumulate within biomolecular condensates formed through phase separation in cells. Here, we use super-resolution imaging to search for chemicals that induce transition chromatin at the microscale. This microscopic screening approach reveals adriamycin (doxorubicin) — a widely used anticancer drug is known interact with specifically induces visible local condensation and global conformational change of cancer primary Hi-C ATAC-seq experiments systematically...
Interphase chromatin compaction well above the 30-nm fiber is documented, but structural motifs underlying this level of folding remain unknown. Taking a reductionist approach, we analyzed in mouse embryonic stem (ES) cells and ES-derived fibroblasts erythroblasts 10–160-megabase pair engineered chromosome regions consisting tandem repeats bacterial artificial chromosomes (BACs) containing ∼200 kilobases mammalian genomic DNA tagged with lac operator (LacO) arrays. Unexpectedly, linear...
Changes in chromosome number impair fitness by disrupting the balance of gene expression. Here we analyze mechanisms to compensate for changes dose that accompanied evolution sex chromosomes from autosomes. Using single-copy transgenes integrated throughout Caenorhabditis elegans genome, show expression all X-linked is balanced between XX hermaphrodites and XO males. However, proximity a dosage compensation complex (DCC) binding site (rex site) neither necessary repress nor sufficient on...
The metabolism and remodeling of alveolar bone are the most active among whole skeletal system, which is related to biological characteristics heterogeneity mesenchymal stromal cells (MSCs). However, there a lack systematic description MSC-derived osteoblastic lineage as well their distinct osteogenic differentiation trajectory bone. In this study, we constructed single-cell atlas mouse through RNA sequencing (scRNA-seq). Remarkably, by comparing cell compositions between long bone,...
Chromosome position effects combined with transgene silencing of multi-copy plasmid insertions lead to highly variable and usually quite low expression levels mini-genes integrated into mammalian chromosomes. Together, these greatly complicate obtaining high-level therapeutic proteins in cells or reproducible individual multiple transgenes. Here, we report a simple, one-step procedure for high-level, mini-gene cells. By inserting at different locations within BAC containing the DHFR...
During vertebrate embryonic development, neural crest-derived ectomesenchyme within the maxillary prominences undergoes precisely coordinated proliferation and differentiation to give rise diverse craniofacial structures, such as tooth palate. However, transcriptional regulatory networks underpinning an intricate process have not been fully elucidated. Here, we perform single-cell RNA-Seq comprehensively characterize dynamics during mouse development from day (E) 10.5-E14.5. Our...