A5038255472- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- RNA Research and Splicing
- Renal and related cancers
- Epigenetics and DNA Methylation
- Robotics and Sensor-Based Localization
- Advanced Image and Video Retrieval Techniques
- RNA modifications and cancer
- Wastewater Treatment and Nitrogen Removal
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Water Quality Monitoring and Analysis
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- Odor and Emission Control Technologies
- Plant-based Medicinal Research
- Biofuel production and bioconversion
- Iron Metabolism and Disorders
Kunming Institute of Zoology
2015-2024
Chinese Academy of Sciences
2015-2024
Northeast Agricultural University
2014-2024
University of Hong Kong
2019-2024
Hangzhou Medical College
2024
Zhejiang Provincial People's Hospital
2024
Pudong New Area People's Hospital
2020-2024
Ningxia Medical University
2024
University of Chinese Academy of Sciences
2019-2024
Sichuan Agricultural University
2021-2024
In vitro development of monkey embryos Owing to technical and ethical limitations, the molecular cellular mechanisms underlying primate gastrulation are far from clear (see Perspective by Tam). Two independent studies used an in culture system study cynomolgus embryo postimplantation up beyond (day 9 day 20). Niu et al. observed vivo morphogenetic events single-cell RNA sequencing chromatin accessibility distinct cell lineages developing embryos. Ma also that key early were recapitulated...
During oogenesis, mammalian eggs accumulate proteins required for early embryogenesis. Although limited data suggest a vital role of these maternal factors in chromatin reprogramming and embryonic genome activation, the full range their functions preimplantation development remains largely unknown. Here we report maintaining chromosome stability euploidy early-cleavage mouse Filia, expressed growing oocytes, encodes protein that binds to MATER participates subcortical complex essential...
Initial cell lineages that presage the inner mass and extra-embryonic trophectoderm are established when eight blastomeres compact to form polarized morulae in preimplantation mouse development. FILIA has been identified as a binding partner MATER (maternal antigen embryos require; also known NLRP5), which is encoded by maternal effect gene. Products of each gene detected growing oocytes and, although transcripts degraded before fertilization, cognate proteins persist early blastocysts. The...
Morphine is frequently used for pain relief, but long-term morphine therapy in patients with chronic results analgesic tolerance and hyperalgesia. There are no effective therapeutic treatments that limit these detrimental side effects. We found pretreatment melatonin could decrease morphine-induced tolerance. was a significant activation of the NLRP3 inflammasome prefrontal cortex peripheral blood morphine-treated mice compared to control animals, which be blocked by melatonin. The induced...
Chinese tree shrews (Tupaia belangeri chinensis) have become an increasingly important experimental animal in biomedical research due to their close relationship primates. An accurately sequenced and assembled genome is essential for understanding the genetic features biology of this animal. In study, we used long-read single-molecule sequencing high-throughput chromosome conformation capture (Hi-C) technology obtain a high-qualitychromosome-scale scaffolding shrew genome. The new reference...
Are active ovarian germ stem cells present in postnatal mouse ovaries under physiological conditions? Active exist and function adult conditions. In vitro studies suggested the existence of mouse, pig human. However, vivo provided evidence against ovaries. Thus, it remains controversial whether such really mammalian Octamer-binding transcription factor 4 (Oct4)-MerCreMer transgenic mice were crossed with R26R-enhanced yellow fluorescent protein (EYFP) to establish a tamoxifen-inducible...
Preimplantation embryogenesis encompasses several critical events including genome reprogramming, zygotic activation (ZGA), and cell-fate commitment. The molecular basis of these processes remains obscure in primates which there is a high rate embryo wastage. Thus, understanding the factors involved reprogramming ZGA might help reproductive success during this susceptible period early development generate induced pluripotent stem cells with greater efficiency. Moreover, explaining...
Recurrent pregnancy loss (RPL) is an important complication in reproductive health. About 50% of RPL cases are unexplained, and understanding the genetic basis essential for its diagnosis prognosis. Herein, we report causal KH domain containing 3 like (KHDC3L) mutations RPL. KHDC3L expressed human epiblast cells ensures their genome stability viability. Mechanistically, binds to poly(ADP-ribose) polymerase 1 (PARP1) stimulate activity. In response DNA damage, also localizes damage sites...
Automatic segmentation of brain tumours using deep learning algorithms is currently one the research hotspots in medical image field. An improved U-Net network proposed to segment improve effect tumours.To solve problems other tumour models such as U-Net, including insufficient ability edge details and reuse feature information, poor extraction location information commonly used binary cross-entropy Dice loss are often ineffective when functions for models, we propose a serial...
Aiming at precise evaluation of the performance soybean seed metering devices, a photoelectric sensor-based real-time monitoring system was designed. The proposed mainly included sensor module for seeding signal collecting, Hall sensors speeding module, microcontroller unit (MCU), light and sound alarm human–machine interface (HMI), other parts. indexes miss, multiples, flow rate, application rate were estimated on basis seeder speed, disk rotation signals, clock signals. These statistics...
DNA repair is essential for maintaining genomic integrity, and may be required in the early embryo to correct damage inherited via gametes, that arises during replication, or response exposure genotoxic agents. The capacity of preimplantation stage mammalian embryos damaged has not been well characterized, particularly primate embryos. In this study, we examined expression 48 mRNAs related sensing different kinds damage, repairing controlling cell cycle provide an opportunity repair. data...
Abstract Background All-trans retinoic acid (RA) is one of the most important morphogens with pleiotropic actions. Its embryonic distribution correlates neural differentiation in developing central nervous system. To explore precise effects RA on mouse stem cells (ESCs), we detected expression nuclear receptors and RA-metabolizing enzymes ESCs investigated roles adherent monolayer culture. Results Upon addition RA, cell was directed rapidly exclusively into lineage. Conversely,...
Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs other regions of genome or genomes have not yet been documented. By exploring non-polyadenylated transcriptomes human, rhesus and mouse, we systematically annotated in all three species. In total, using available data a limited set cell lines, 19 identified tissue- species-specific expression patterns....
Gene knockout has been used as a common strategy to improve microbial strains for producing chemicals. Several algorithms are available predict the target reactions be deleted. Most of them apply mixed integer bi-level linear programming (MIBLP) based on metabolic networks, and use duality theory transform optimization problem large-scale MIBLP single-level programming. However, validity transformation was not proved. Solution depends structure inner problem. If is continuous,...
BackgroundSchizophrenia is a complex mental disorder resulting in poor life quality and high social economic burden. Despite the fact that genome-wide association studies (GWASs) have successfully identified number of risk loci for schizophrenia, identifying causal genes at elucidating their roles disease pathogenesis remain major challenges.MethodsThe summary data-based Mendelian randomization analysis (SMR) was used to integrate large-scale GWAS schizophrenia with brain expression...
Unmanned aerial vehicle (UAV) ad hoc networks can be deployed flexibly and rapidly without any infrastructure, so as to play an irreplaceable role in postdisaster relief. However, the dynamic topology energy constraints of UAVs always bring new challenges design effective network architectures routing strategies. In this article, a three-layer UAV architecture is constructed, channel models for different layers are discussed categorically. A greedy perimeter comprehensive evaluation (GCER)...