A5057064387- Reproductive Biology and Fertility
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Pregnancy and preeclampsia studies
- Renal and related cancers
- Genomic variations and chromosomal abnormalities
- TGF-β signaling in diseases
- Ovarian cancer diagnosis and treatment
- Gynecological conditions and treatments
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Syndromes and Imprinting
- Growth Hormone and Insulin-like Growth Factors
- Chromosomal and Genetic Variations
- Hypothalamic control of reproductive hormones
First Affiliated Hospital of Zhengzhou University
2016-2025
Zhengzhou University
2009-2022
National Clinical Research Center for Digestive Diseases
2021
Institute of Biophysics
2016
Chinese Academy of Sciences
2016
Seattle Reproductive Medicine
2014
5-methylcytosine (m5C) is a post-transcriptional RNA modification identified in both stable and highly abundant tRNAs rRNAs, mRNAs. However, its regulatory role mRNA metabolism still largely unknown. Here, we reveal that m5C modification enriched CG-rich regions immediately downstream of translation initiation sites has conserved, tissue-specific dynamic features across mammalian transcriptomes. Moreover, formation mRNAs mainly catalyzed by the methyltransferase NSUN2, specifically recognized...
Recently, two patients with primary ovarian insufficiency (POI) delivered healthy babies after in vitro activation (IVA) treatment followed by auto-transplantation of frozen-thawed tissues.This study sought to report the first case live birth IVA following fresh tissue grafting POI, together monitoring follicle development and serum hormonal changes.This was a prospective observational cohort study.We performed 14 POI mean age 29 years, duration since last menses 3.8 average basal FSH level...
N6-methyladenosine (m6A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis embryogenesis. However, the landscape function of m6A at single cell resolution have not extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell sequencing (scm6A-seq) method simultaneously profile methylome transcriptome oocytes/blastomeres cleavage-stage embryos. We found that deficiency leads aberrant...
Background: The sperm DNA fragmentation index (DFI) is widely regarded as a key measure for assessing male fertility, but the predictive value of DFI outcomes assisted reproductive technology (ART) remains under debate. In this study, we used large sample to analyze effect on pregnancy following ART and its relationship with oocyte fertilization embryo development in vitro (IVF)/intracytoplasmic injection (ICSI). We also explore (SDF) associated factors fertility. Methods: between measured...
Abstract Pre-eclampsia is a pregnancy-related disease that may cause maternal, neonatal and fetal morbidity mortality exists in 3–5% of pregnancies worldwide. The discovery dysregulated microRNAs their roles placental development has provided new avenue for elucidating the mechanism involved this pregnancy-specific disorder. Here, human miR-181a-5p, microRNA increased both plasma placenta severe pre-eclamptic patients, invasion migration trophoblasts were investigated. Ectopic-expression...
Introduction Age, polycystic ovary syndrome (PCOS), low body mass index (BMI), high antral follicle count (AFC), increased anti-Muller hormone (AMH) levels, and elevated serum estradiol (E2) concentrations are risk factors for ovarian hyperstimulation (OHSS). However, data on the relationship between OHSS severity in patients with PCOS rare. Objective This retrospective study examined their effect undergoing vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). Method The...
Abstract The ovary ages earlier than most other tissues, yet the underlying mechanisms remain elusive. Here a comprehensive analysis of transcriptomic landscapes in different organs young and middle-aged mice revealed that ovaries showed expression age-associated genes, identifying increased NADase CD38 decreased NAD + levels mice. Bulk single-cell RNA sequencing deletion mitigated ovarian aging, preserving fertility follicle reserve aged by countering age-related gene changes intercellular...
Recent advances highlight the pivotal role of nicotinamide adenine dinucleotide (NAD+ ) in ovarian aging. However, roles de novo NAD+ biosynthesis on aging are still unknown. Here, we found that genetic ablation Ido1 (indoleamine-2,3-dioxygenase 1) or Qprt (Quinolinate phosphoribosyl transferase), two critical genes biosynthesis, resulted decreased levels middle-aged mice, leading to subfertility, irregular estrous cycles, reduced reserve, and accelerated Moreover, observed impaired oocyte...
ABSTRACT Recent studies have shown that disruptions in the nicotinamide adenine dinucleotide (NAD + ) de novo synthesis pathway accelerate ovarian aging, yet its role spermatogenesis remains largely unknown. In this study, we investigated impact of NAD on by generating Qprt ‐deficient mice using CRISPR‐Cas9 to target quinolinate phosphoribosyl transferase ( ), a key enzyme predominantly expressed spermatocytes. Our results revealed deletion did not affect levels or testes 3‐month‐old mice....
Are there any correlations between the phenotypes of polycystic ovary syndrome (PCOS) and genotypes PCOS susceptibility single nucleotide polymorphisms (SNPs) in THADA, DENND1A LHCGR? The genes, THADA DENND1A, carry risk alleles that are associated with endocrine metabolic disturbances patients PCOS. is a heterogeneous endocrinopathy characterized by oligo-anovulation, hyperandrogenism ovaries. In previous genome-wide association study, SNP variants rs13429458, rs12478601, rs2479106,...
What is the relationship between telomere length in sperm and early embryonic development vitro fertilization (IVF)? Sperm (STL) positively associated with embryo quality IVF. Previous studies have shown that STL differs among human males. In order to determine associations STL, laboratory parameters clinical pregnancy IVF, 418 couples were recruited from August 2013 2014. We collected semen samples used quantitative PCR technique detect mean for each patient. These data compared IVF...
Regulation of progesterone production in granulosa cells is important for normal reproductive functions. Steroidogenic acute regulatory protein (StAR) recognized as the key involved rate-limiting step steroidogenesis. TGF-β1 detected human follicular fluid, and its receptors are expressed cells. However, functional role regulation StAR expression remains unknown.Our objective was to investigate effects on cells.SVOG that were obtained from women undergoing vitro fertilization immortalized...
The quality of postovulatory metaphase II oocytes undergoes a time-dependent deterioration as result the aging process. Melatonin is considered to be an anti-aging agent. However, underlying mechanisms how melatonin improves aged remain largely unclear. In this study, by using mouse model, we found that there were elevated reactive oxygen species levels and impaired mitochondrial function demonstrated reduced membrane potential increased aggregation in 24 h, accompanied number meiotic...
Abstract Polycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine disorder characterized by ovulation dysfunction, androgen excess, ovarian polycystic changes, insulin resistance, and infertility. Although underlying mechanisms for PCOS are still unknown, inflammation mitochondrial dysfunction in granulosa cells (GCs) of patients have been reported. Here, we found that Nicotinamide Adenine Dinucleotide (NAD+) levels GCs was significantly decreased when compared with controls....