A5101743793- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Ovarian function and disorders
- Genomic variations and chromosomal abnormalities
- Biotin and Related Studies
- Genetic and Kidney Cyst Diseases
- Genetic factors in colorectal cancer
- Reproductive Health and Technologies
- Immunodeficiency and Autoimmune Disorders
- Pluripotent Stem Cells Research
- Digital Transformation in Industry
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Histone Deacetylase Inhibitors Research
- Urological Disorders and Treatments
- Genetic Syndromes and Imprinting
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Medical Imaging and Pathology Studies
- RNA Interference and Gene Delivery
- Enzyme Structure and Function
First Affiliated Hospital of Zhengzhou University
2014-2025
Shanghai Jiao Tong University
2020-2023
Zhengzhou University
2008
Histone modifications regulate gene expression and development. To address how they are reprogrammed in human early development, we investigated key histone marks oocytes embryos. Unlike that mouse oocytes, the permissive mark trimethylated H3 lysine 4 (H3K4me3) largely exhibits canonical patterns at promoters oocytes. After fertilization, prezygotic genome activation (pre-ZGA) embryos acquire chromatin widespread H3K4me3 CpG-rich regulatory regions. By contrast, repressive H3K27me3...
Abstract Clinically discarded human embryos, which are generated from both normal and abnormal fertilizations, have the potential of developing into blastocysts. A total 1,649 including zygotes containing (2PN) (0PN, 1PN, 3PN ≥4PN) pronuclei prematurely cleaved embryos (2Cell), were collected for in vitro culture to investigate their developmental chromosomal constitution using an SNP array-based analysis. We found that blastocyst formation rates 63.8% (for 2Cell embryos), 22.6% (2PN), 16.7%...
Human mutL homologl (MLH1) works coordinately in sequential steps to initiate repair of DNA mismatches, and aberrant MLH1 expression is related spermatogenetic malfunction. In the present study, patients with azoospermia was investigated, moderating effects miR-188-3p on spermatogenesis were identified.Testicular tissues from 16 obstructive (OA) non-obstructive (NOA), eight healthy collected. Real-time PCR, Western blotting immunohistochemical staining used detect expression. Chromatin...
Abstract The short‐chain dehydrogenase/reductase (SDR) superfamily members acyl‐ACP reductases FabG and FabI are indispensable core enzymatic modules catalytic orientation controllers in type‐II fatty acid biosynthesis. Herein, we report their distinct substrate allosteric recognition enantioselective reduction mechanisms. achieves regulation of ACP NADPH through binding across two adjacent monomers, while follows an irreversible compulsory order that NADH must precede on a discrete monomer....
Severe combined immunodeficiency (SCID) is a set of rare monogenic inherited diseases that together represent the most severe form primary disease phenotype. Preimplantation genetic testing for defects (PGT-M) an effective reproductive technology strategy to prevent disease-causing gene mutations from being transmitted offspring. The aim this study was report use PGT-M based on karyomapping in four families avoid birth SCID children. Four couples underwent due SCID. started with biopsy...
As one of the non-classical major histocompatibility complex(MHC)-1 antigens, Human Leukocyte Antigen G (HLA-G), has been suggested as a prognostic marker to identify embryo developmental potential. In present study, we investigated potential roles HLA-G in human spermatogenesis and early embryonic development. Quantitative real-time PCR analysis revealed that HLA-G's expression was increased with Johnsen score testicular tissues. There no significant difference mRNA between tissues 8–9...
Peutz Jeghers syndrome (PJS) is an autosomal dominant genetic disorder caused by STK11 mutation with a predisposition to gastrointestinal polyposis and cancer. PJS patients suffer poor quality of life are highly concerned about whether deleterious mutations transmit their offspring. Therefore, this study aimed propose feasible clinical management provide effective preimplantation testing for monogenic defect (PGT-M) strategies protect offspring from inheriting the disease.A hospital-based...
Uniparental embryos derived from only the mother (gynogenetic [GG]) or father (androgenetic [AG]) are unique models for studying genomic imprinting and parental contributions to embryonic development. Human parthenogenetic can be obtained following artificial activation of unfertilized oocytes, but production AG by injection two sperm into one denucleated oocyte leads an extra centriole, resulting in multipolar spindles, abnormal cell division, developmental defects. Here, we improved...
Is it possible to predict blastocyst quality, embryo chromosomal ploidy, and clinical pregnancy outcome after single transfer from developmental morphokinetic parameters? The parameters of 1011 blastocysts 227 patients undergoing preimplantation genetic testing were examined. Correlations between the quality blastocysts, outcomes following retrospectively analyzed. embryos in high-quality group significantly shorter than those low-quality (p < 0.05). In contrast, CC2 time was prolonged On...
Objective To investigate CT and MRI features of hepatic sclerosed hemangioma (HSH). Methods CT findings were retrospectively reviewed in 20 cases HSH, all which confirmed pathologically after surgery. Twenty patients underwent scan,4 MRI. Meanwhile, the enhancement pattern signal intensity analyzed either. Results Twenty showed main part tumor was hypo-attenuating on plain scanning, 16 central area markedly more scanning. After administration intravenous contrast media,...
Abstract Background: Achievement of a live birth is the marker success in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatments. For patients with polycystic ovary syndrome (PCOS) who are undergoing these treatments, some predictive models and nomograms have been published. However, further development required for their useful application clinical settings. Method: To establish validate prediction model nomogram to predict rate women PCOS IVF/ICSI. Records on 1193...