A5084043651- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Genomic variations and chromosomal abnormalities
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Gynecological conditions and treatments
- Ovarian cancer diagnosis and treatment
- Chromosomal and Genetic Variations
- Phenothiazines and Benzothiazines Synthesis and Activities
- RNA modifications and cancer
- Synthesis and Characterization of Heterocyclic Compounds
- Reproductive Health and Contraception
- Congenital Anomalies and Fetal Surgery
- Mesenchymal stem cell research
- Ectopic Pregnancy Diagnosis and Management
- Uterine Myomas and Treatments
- NF-κB Signaling Pathways
- Genetic Syndromes and Imprinting
Beijing Obstetrics and Gynecology Hospital
2019-2024
Capital Medical University
2019-2024
National Natural Science Foundation of China
2023
Beijing Tongren Hospital
2019
First Affiliated Hospital of Zhengzhou University
2004-2016
Zhengzhou University
2011-2013
Huazhong University of Science and Technology
2004
Abstract Clinically discarded human embryos, which are generated from both normal and abnormal fertilizations, have the potential of developing into blastocysts. A total 1,649 including zygotes containing (2PN) (0PN, 1PN, 3PN ≥4PN) pronuclei prematurely cleaved embryos (2Cell), were collected for in vitro culture to investigate their developmental chromosomal constitution using an SNP array-based analysis. We found that blastocyst formation rates 63.8% (for 2Cell embryos), 22.6% (2PN), 16.7%...
Abstract Chemotherapeutic agents are extensively used to treat malignancies. However, chemotherapy-induced ovarian damage and reduced fertility severe side effects. Recently, stem cell transplantation has been reported be an effective strategy for premature insufficiency (POI) treatment, but safety can still issue in cell-based therapy. Here, we show the protective effects of human umbilical cord mesenchymal cell-derived conditioned medium (hUCMSC-CM) on a cisplatin (Cs)-induced injury...
The development of an effective oocyte cryopreservation system will have a significant impact on the clinical practice reproductive medicine. However, important option emergency has yet to be well documented. In this report, we review cases 15 women with male partners who were diagnosed nonobstructive azoospermia and for whom testicular sperm extraction day retrieval failed. Emergency vitrification was performed after two months, vitrified oocytes warmed surviving inseminated frozen-thawed...
Abstract Background Women with endometriosis and previous cystectomy may respond less well to gonadotropin stimulation, which results in fewer oocytes retrieved poor pregnancy outcomes. Choosing an appropriate protocol for such populations is essential. This study involved analysis of the effect different controlled ovarian stimulation (COS) protocols on clinical outcomes vitro fertilization-embryo transfer (IVF-ET) women diminished reserve (DOR) who underwent endometrioma cystectomy....
We successfully performed preimplantation genetic diagnosis (PGD) and simultaneous screening (PGS) using single nucleotide polymorphism (SNP) microarrays for couples with balanced chromosome rearrangements in China. A total of 428 molecular karyotypes were diagnosed from 62 undergoing 68 vitro fertilization (IVF) cycles. Of these, 48.1% the embryos chromosomally normal without translocation errors or aneuploidy. embryos, 18.0% euploid, but imbalanced due to transmission derivatives. 6.5% had...
Several studies have reported improved IVF by shortening the time of sperm-oocyte coincubation from 16-18 hours to 1-4 hours. The objective this study was examine advantages and disadvantages a shortened in order assess effects insemination method for clinical practice. Two methods, (4 hours) standard (16-18 sperm-oocytes two groups patients based on quality sperm were compared. Group I, composed couples without male factor; II, involved with mild factor. Fertilization, good embryos,...
Premature ovarian insufficiency (POI), which is defined as loss of function that occurs before the age 40, causes menstrual disturbances, infertility, and diverse health problems in females. Despite limited understanding molecular basis underlying POI pathology, we had previously demonstrated cooperation miR-106a FBXO31 plays a pivotal role diminished reserve (DOR), with serving putative target miR-106a. In this study, found aberrantly expressed granulosa cells patients, leading to...
Abstract Purpose Problems with fallopian tubes are one of the main reasons for women to undergo in vitro fertilization-embryo transfer (IVF-ET). A large proportion ectopic pregnancy, tube obstruction and hydrosalpinx have had or both removed by salpingectomy. With increasing age, ovarian reserve deteriorates, numbers retrieved oocytes, available embryos high-quality reduced, live birth rate treated IVF treatment is affected. Thus, it important understand how salpingectomy affects rates...
Objective To evaluate the effects on pregnancy outcome of freezing time from oocyte retrieval and thawing method for metaphase Ⅱ human oocytes vitrification. Methods From Mar 2007 to 2009, clinical 30 infertile women undergoing vitrified-thawing in vitro fertilizationembryo transfer(IVF-ET) Reproductive Medical Center First Affiliated Hospital Zhengzhou University was studied retrospectively, including 21 with double fallopian tube obstruction 9 women's husband azoospermia. All were divided...
Abstract Better pregnancy outcomes can be obtained by human mature oocyte vitrification, but many problems remain to resolved in vitrification. Since development possesses its own maturity cycle, there should the optimal timing for The purpose of this study was observe effects frozen on spindle density, angle between polar body and spindle, embryo intracytoplasmic sperm injection (ICSI) vitrified mouse oocytes explore possible mechanism. Mouse were randomly divided into three groups...
Objectives To investigate the effects of day 5 embryo transfer (D5ET) compared with 3 (D3ET) in patients at high risk developing ovarian hyperstimulation syndrome (OHSS); to analyse factors affecting blastocyst formation. Methods Patients OHSS underwent either D3ET or D5ET. Results A total 253 received D3ET; 263 The number embryos transferred was lower D5ET group than group. There were no between-group differences pregnancy live birth rates. Implantation rate higher, and multifetation lower,...
Preserving fertility is a vital concern for young women diagnosed with endometrial carcinoma. The clinical management of such patients often disappointing. It rare to have two consecutive successful pregnancies. We present child-bearing-age woman who underwent preservation therapy due Following therapy, she in vitro fertilization and embryo transfer. After receiving her first fresh transfer, successfully conceived gave birth healthy child. Two years after the transfer regular follow-up, had...
Objective To investigate the role of sperm fluorescence in situ hybridization (FISH) preimplantation genetic diagnosis (PGD) for male chromosomal disorders. Methods From Jul. 2006 to Aug. 2008, FISH was performed and embryo 9 infertile couples due abnormality including 7 with Robertsonian translocation, one couple reciprocal translocation Klinefelter's syndrome. Correlation analysis between results. Results (1) 8568 sperms showed 24 had no signals. The rate normal/balanced carders were...
Abstract Genome-wide interactions between RNA-binding proteins (RBPs) and RNA targets account for an important portion of post-transcriptional regulation. IGF2BP2 is associated with type 2 diabetes (T2D) obesity reportedly functions as RBP that regulates a series target genes by binding transcripts. In this study, we detected the differential expression in granulosa cells from women ovarian disorders performed RNA-seq RIP-seq experiments immortalized human (KGN cells) to evaluate global...