Application of sperm fluorescence in situ hybridization in preimplantation genetic diagnosis

• DOI: 10.3760/cma.j.issn.0529-567x.2009.06.005
• Publication Date: 2009-06-01
• Authors: Gang Li; Yingpu Sun; Haixia Jin; Zhimin Xin; Shanjun Dai
• Keywords: Preimplantation Genetic Diagnosis; Robertsonian translocation

Objective To investigate the role of sperm fluorescence in situ hybridization (FISH) preimplantation genetic diagnosis (PGD) for male chromosomal disorders. Methods From Jul. 2006 to Aug. 2008, FISH was performed and embryo 9 infertile couples due abnormality including 7 with Robertsonian translocation, one couple reciprocal translocation Klinefelter's syndrome. Correlation analysis between results. Results (1) 8568 sperms showed 24 had no signals. The rate normal/balanced carders were 85.71% (6045/7053)in seven 30.42% (306/1006) 68.76% (350/509) Klinefeher's (2) A total 158 embryos biopsied, which 135 successfully fixed FISH. hundred exhibit informative signal 36 75 abnormal embryos. Twenty-one transferred obtained successful term pregnancy. 29.0% (31/107) carriers 6.3% (1/16) 33.3% (4/12) Klinefeher' s (3) positive correlated relationship found percentage normal (r= 0.75,P=0.02). Conclusion It is advisable recommend being routinely incorporated into screening offered prior diagnosis.

Key words: Preimplantation diagnosis; Chromosome aberrations; In hybridization; fluorescence; Spermatozoa