A5032836116- TGF-β signaling in diseases
- Reproductive Biology and Fertility
- Connective Tissue Growth Factor Research
- Kruppel-like factors research
- Fibroblast Growth Factor Research
- Ovarian function and disorders
- Hippo pathway signaling and YAP/TAZ
- Estrogen and related hormone effects
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Bone Metabolism and Diseases
- Ovarian cancer diagnosis and treatment
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Growth Hormone and Insulin-like Growth Factors
- Renal and related cancers
- Connexins and lens biology
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Urological Disorders and Treatments
- GDF15 and Related Biomarkers
- Mesenchymal stem cell research
- Biomarkers in Disease Mechanisms
- Hypothalamic control of reproductive hormones
First Affiliated Hospital of Zhengzhou University
2020-2025
Zhengzhou University
2023
Kaohsiung Municipal Hsiao-Kang Hospital
2022
Kaohsiung Medical University
2022
University of British Columbia
2010-2019
British Columbia Children's Hospital
2017-2019
Terry Fox Research Institute
2018-2019
BC Cancer Agency
2017-2018
National Chiayi University
2017
Child and Family Research Institute
2010-2016
Optimal treatment remains uncertain for patients with cognitive impairment that persists or returns after standard IV antibiotic therapy Lyme disease.Patients had well-documented disease, at least 3 weeks of prior antibiotics, current positive IgG Western blot, and objective memory impairment. Healthy individuals served as controls practice effects. Patients were randomly assigned to 10 double-masked ceftriaxone placebo then no therapy. The primary outcome was neurocognitive performance week...
In addition to somatic cell-derived growth factors, oocyte-derived differentiation factor (GDF)9 and bone morphogenetic protein (BMP)15 play essential roles in female fertility. However, few studies have investigated their effects on human ovarian steroidogenesis, fewer still examined differential or underlying molecular determinants. the present study, we used immortalized granulosa cells (SVOG) cell tumor (KGN) compare of GDF9 BMP15 steroidogenic enzyme expression investigate potential...
Regulation of progesterone production in granulosa cells is important for normal reproductive functions. Steroidogenic acute regulatory protein (StAR) recognized as the key involved rate-limiting step steroidogenesis. TGF-β1 detected human follicular fluid, and its receptors are expressed cells. However, functional role regulation StAR expression remains unknown.Our objective was to investigate effects on cells.SVOG that were obtained from women undergoing vitro fertilization immortalized...
In the ovary, connexin-coupled gap junctions in granulosa cells play crucial roles follicular and oocyte development as well corpus luteum formation. Our previous work has shown that theca cell-derived bone morphogenetic protein (BMP)4 BMP7 decrease junction intercellular communication (GJIC) activity via down-regulation of connexin43 (Cx43) expression immortalized human cells. However, effects oocyte-derived growth factors on Cx43 remain to be elucidated. The present study was designed...
Abstract Dysregulation of the Hippo pathway in liver results overgrowth and eventually tumorigenesis. To date, several upstream mechanisms have been identified that affect pathway, which ultimately regulate YAP, major downstream effector pathway. However, regulators remain poorly defined. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite has shown to stimulate hepatocellular carcinoma (HCC) cell proliferation, but whether involved S1P-stimulated HCC proliferation remains...
Cell‐fate determination is influenced by interactions between master transcription factors (TFs) and cis‐regulatory elements. Hepatocyte nuclear factor 4 alpha (HNF4A), a liver‐enriched TF, acts as controller in specification of hepatic progenitor cells regulating network TFs to control onset hepatocyte cell fate. Using analysis genome‐wide histone modifications, DNA methylation, hydroxymethylation mouse hepatocytes, we show that HNF4A occupies active enhancers hepatocytes essential for...
In ovarian cancer, it has been shown that E-cadherin is down-regulated by epidermal growth factor (EGF) receptor (EGFR) activation, and cells with low expression are particularly invasive. Although generally believed reactive oxygen species play important roles in intracellular signal transduction, the role of EGF-mediated reductions remains to be elucidated. this study, we show EGF treatment up-regulating its transcriptional repressors, Snail Slug, human cancer cells. Using...
In high-grade ovarian cancer cultures, it has been shown that epidermal growth factor (EGF) induces cell invasion by activating an epithelial-mesenchymal transition (EMT). However, the effect of EGF on serous borderline tumors (SBOT) and low-grade carcinomas (LGC) remains unknown. Here, we show receptor (EGFR) was expressed, treatment increased migration in two cultured SBOT lines, SBOT3.1 SV40 large T antigen-infected cells (SBOT4-LT), LGC MPSC1 LT/ST-immortalized (ILGC). induced...
Inhibin β-subunits and activin receptors are expressed by human trophoblast cells. Although A has been shown to enhance cell invasion, whether two additional isoforms, B AB, exert similar effects remains unknown. Moreover, the expression of mesenchymal adhesion molecule neural cadherin (N-cadherin) is essential for this proinvasive effect yet be determined.To examine all three isoforms on invasion involvement N-cadherin.HTR8/SVneo immortalized extravillous cytotrophoblast cells primary...
Elevated expression of cyclooxygenase 2 (COX2 (PTGS2)) has been reported to occur in human ovarian cancer and be associated with poor prognosis. We have previously demonstrated that COX2-derived prostaglandin E2 (PGE2) promotes cell invasion. had also epidermal growth factor (EGF) induces invasion by downregulating the E-cadherin through various signaling pathways. However, it remains unclear whether COX2 PGE2 are involved EGF-induced downregulation cells. In this study, we showed EGF...
Does transforming growth factor-β1 (TGF-β1) up-regulate connexin43 (Cx43) to promote cell–cell communication in human granulosa cells? TGF-β1 up-regulates Cx43 and increases gap junction intercellular activities (GJIC) cells, this effect occurs via the activin receptor-like kinase (ALK)5-mediated Sma- Mad-related protein (SMAD)2/3-SMAD4-dependent pathway. its receptors are expressed follicular fluid contains protein. In junctions play an important role development of follicles oocytes. This...
Aberrant regulation of ovulation is one the major causes infertility. In animal models, 3 epidermal growth factor (EGF)-like factors, amphiregulin (AREG), betacellulin (BTC), and epiregulin (EREG), have been shown to be involved in by regulating cyclooxygenase-2 (COX-2) expression prostaglandin E2 (PGE2) production. However, whether same true humans remains largely unknown. Our objective was investigate effects AREG, BTC, EREG on COX-2 PGE2 production human granulosa cells. SVOG cells are...
Cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) production have been shown to play key roles in the regulation of ovulation. The TGF-β superfamily members are important molecules that regulate many ovarian functions under normal physiological pathological conditions. TGF-β1 its receptors expressed human granulosa cells. However, date, whether can COX-2 PGE2 production, which turn contribute process ovulation, remains unknown.The objective study was investigate effects on...
Exerting a broad range of biological effects in various tissues, activins are homo- or heterodimers activin/inhibin β-subunits (βA, βB, βC, and βE humans). Although A (βAβA), B (βBβB), AB (βAβB), AC (βAβC) have been demonstrated the female reproductive system, little is known about their individual functions ovary.To investigate roles activities regulating steroidogenesis human granulosa cells.Human granulosa-lutein cells obtained from 32 patients undergoing vitro fertilization were used to...
Connective tissue growth factor (CTGF), which is also called CCN2, a secreted matricellular protein. CTGF regulates various important cellular functions by interacting with multiple molecules in the microenvironment. In ovary, mainly expressed granulosa cells and involved regulation of follicular development, ovulation luteinization. TGF-β1 has been shown to up-regulate expression rat hen cells. However, underlying molecular mechanisms this up-regulation remain undefined. More importantly,...
Abstract Ovarian hyperstimulation syndrome (OHSS) is one of the most serious and iatrogenic complications that can occur during in vitro fertilization treatment. Although pathogenesis OHSS not fully understood, vascular endothelial growth factor (VEGF) has been recognized as an important mediator development OHSS. Transforming factor-beta-1 (TGF-β1) known to regulate various ovarian functions. However, whether VEGF be regulated by TGF-β1 human granulosa cells determined. In addition, role...
Extravillous trophoblast (EVT) cell invasion is a tightly regulated process that requires for normal pregnancy. The epithelial-mesenchymal transition (EMT) has been implicated in EVT invasion. Growth differentiation factor-8 (GDF-8), member of the transforming growth factor-beta (TGF-β) superfamily, expressed human placenta and promotes by upregulating expression matrix metalloproteinase 2 (MMP2). However, underlying molecular mechanism GDF-8-induced MMP2 remains undetermined. Therefore,...
Apoptosis in ovarian surface epithelial (OSE) cells is induced by transforming growth factor-beta (TGF-β). However, high-grade serous carcinomas (HGC) are refractory to the inhibitory functions of TGF-β; their invasiveness up-regulated TGF-β through epithelial-mesenchymal transition (EMT) activation. Serous borderline tumors (SBOT) have been recognized as distinct entities that give rise invasive low-grade (LGC), which a relatively poor prognosis and unrelated HGC. While it not fully...