A5102708873- Reproductive Biology and Fertility
- TGF-β signaling in diseases
- Ovarian function and disorders
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Connective Tissue Growth Factor Research
- Kruppel-like factors research
- Pregnancy and preeclampsia studies
- Biomarkers in Disease Mechanisms
- Bone Metabolism and Diseases
- Connexins and lens biology
- Renal and related cancers
- Hypothalamic control of reproductive hormones
- Urological Disorders and Treatments
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Ovarian cancer diagnosis and treatment
- Heat shock proteins research
- Fibroblast Growth Factor Research
- Prenatal Screening and Diagnostics
- Hippo pathway signaling and YAP/TAZ
- MicroRNA in disease regulation
China Medical University
2022-2025
China Medical University Hospital
2022-2025
China Medical University
2022-2024
University of Chicago
2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2024
Universidad de Los Andes, Chile
2024
Experimental Medicine and Biology Institute
2024
Second Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Universidad Maimónides
2024
Mechanisms of implantation such as determination the attachment pole, fetal-maternal communication, and underlying causes failure are largely unexplored. Here, we performed single-cell RNA sequencing on peri-implantation embryos from both humans mice to explore trophectoderm (TE) development embryo-endometrium cross-talk. We found that transcriptomes polar mural TE diverged after hatched zona pellucida in species, with being more mature than TE. The poles show similarities cell cycle...
In addition to somatic cell-derived growth factors, oocyte-derived differentiation factor (GDF)9 and bone morphogenetic protein (BMP)15 play essential roles in female fertility. However, few studies have investigated their effects on human ovarian steroidogenesis, fewer still examined differential or underlying molecular determinants. the present study, we used immortalized granulosa cells (SVOG) cell tumor (KGN) compare of GDF9 BMP15 steroidogenic enzyme expression investigate potential...
Regulation of progesterone production in granulosa cells is important for normal reproductive functions. Steroidogenic acute regulatory protein (StAR) recognized as the key involved rate-limiting step steroidogenesis. TGF-β1 detected human follicular fluid, and its receptors are expressed cells. However, functional role regulation StAR expression remains unknown.Our objective was to investigate effects on cells.SVOG that were obtained from women undergoing vitro fertilization immortalized...
In the ovary, connexin-coupled gap junctions in granulosa cells play crucial roles follicular and oocyte development as well corpus luteum formation. Our previous work has shown that theca cell-derived bone morphogenetic protein (BMP)4 BMP7 decrease junction intercellular communication (GJIC) activity via down-regulation of connexin43 (Cx43) expression immortalized human cells. However, effects oocyte-derived growth factors on Cx43 remain to be elucidated. The present study was designed...
Cryopreservation causes cryoinjury to oocytes and impairs their developmental competence. Melatonin (MLT) can improve the effect of cryopreservation in animal oocytes. However, no such studies on human have been reported. In this study, collected vitro-matured were randomly divided into following groups: fresh group, MLT-treated (MC) no-MLT-treated (NC) group. After vitrification warming, viable from these three groups assessed for mitochondrial function, ultrastructure, permeability...
Polycystic ovary syndrome (PCOS) is a complex disorder associated with multiple metabolic disturbance, including defective glucose metabolism and insulin resistance. The altered metabolites caused by the related disturbance may affect ovarian follicles, which can be reflected in follicular fluid composition. aim of this study to investigate profiles women PCOS using an advanced sequential window acquisition all theoretical fragment-ion spectra (SWATH) mass spectrometry. Nineteen twenty-one...
Elevated expression of cyclooxygenase 2 (COX2 (PTGS2)) has been reported to occur in human ovarian cancer and be associated with poor prognosis. We have previously demonstrated that COX2-derived prostaglandin E2 (PGE2) promotes cell invasion. had also epidermal growth factor (EGF) induces invasion by downregulating the E-cadherin through various signaling pathways. However, it remains unclear whether COX2 PGE2 are involved EGF-induced downregulation cells. In this study, we showed EGF...
Does transforming growth factor-β1 (TGF-β1) up-regulate connexin43 (Cx43) to promote cell–cell communication in human granulosa cells? TGF-β1 up-regulates Cx43 and increases gap junction intercellular activities (GJIC) cells, this effect occurs via the activin receptor-like kinase (ALK)5-mediated Sma- Mad-related protein (SMAD)2/3-SMAD4-dependent pathway. its receptors are expressed follicular fluid contains protein. In junctions play an important role development of follicles oocytes. This...
Aberrant regulation of ovulation is one the major causes infertility. In animal models, 3 epidermal growth factor (EGF)-like factors, amphiregulin (AREG), betacellulin (BTC), and epiregulin (EREG), have been shown to be involved in by regulating cyclooxygenase-2 (COX-2) expression prostaglandin E2 (PGE2) production. However, whether same true humans remains largely unknown. Our objective was investigate effects AREG, BTC, EREG on COX-2 PGE2 production human granulosa cells. SVOG cells are...
Exerting a broad range of biological effects in various tissues, activins are homo- or heterodimers activin/inhibin β-subunits (βA, βB, βC, and βE humans). Although A (βAβA), B (βBβB), AB (βAβB), AC (βAβC) have been demonstrated the female reproductive system, little is known about their individual functions ovary.To investigate roles activities regulating steroidogenesis human granulosa cells.Human granulosa-lutein cells obtained from 32 patients undergoing vitro fertilization were used to...
Connective tissue growth factor (CTGF), which is also called CCN2, a secreted matricellular protein. CTGF regulates various important cellular functions by interacting with multiple molecules in the microenvironment. In ovary, mainly expressed granulosa cells and involved regulation of follicular development, ovulation luteinization. TGF-β1 has been shown to up-regulate expression rat hen cells. However, underlying molecular mechanisms this up-regulation remain undefined. More importantly,...
Cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) production have been shown to play key roles in the regulation of ovulation. The TGF-β superfamily members are important molecules that regulate many ovarian functions under normal physiological pathological conditions. TGF-β1 its receptors expressed human granulosa cells. However, date, whether can COX-2 PGE2 production, which turn contribute process ovulation, remains unknown.The objective study was investigate effects on...