A5041022359- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Advanced Glycation End Products research
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Sperm and Testicular Function
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Prenatal Screening and Diagnostics
- Seed Germination and Physiology
- Circadian rhythm and melatonin
- Pregnancy and Medication Impact
- Pregnancy and preeclampsia studies
- Adenosine and Purinergic Signaling
- Diabetes and associated disorders
- Genetics, Aging, and Longevity in Model Organisms
- Eosinophilic Esophagitis
- Kruppel-like factors research
- Fatty Acid Research and Health
- Cell death mechanisms and regulation
- Orbital Angular Momentum in Optics
- Birth, Development, and Health
- Pregnancy-related medical research
Anhui Medical University
2019-2025
First Affiliated Hospital of Anhui Medical University
2019-2025
Ministry of Education of the People's Republic of China
2014-2024
National Health and Family Planning Commission
2023
Huazhong University of Science and Technology
2014-2021
Dongguan People’s Hospital
2021
Shandong First Medical University
2011-2016
Air Force Institute of Aviation Medicine Affiliated Hospital
2015
Recurrent spontaneous abortion (RSA) is a common complication of pregnancy that affects the physical and mental health pregnant women, approximately 50% mechanisms are unclear. Our previous studies have found high mobility group box 1 (HMGB1) molecules highly expressed at maternal-fetal interface unexplained recurrent (URSA) patients. The purpose this study was to further detect expression HMGB1 pyroptosis in decidual tissue URSA patients, explore potential mechanism protective role patients...
Melatonin (MT) regulates reproductive performance as a potent antioxidant; however, its beneficial effects on oocyte development remain largely unknown, especially in human oocytes. The collected 193 immature oocytes from the controlled ovarian hyperstimulation (COH) cycle underwent vitro maturation (IVM) IVM medium contained 10 μmol/L MT (n = 105, M group) and no 88, NM group), followed by insemination embryo culture. results showed that high-quality blastocyst formation rate group (28.4%)...
Cryopreservation causes cryoinjury to oocytes and impairs their developmental competence. Melatonin (MLT) can improve the effect of cryopreservation in animal oocytes. However, no such studies on human have been reported. In this study, collected vitro-matured were randomly divided into following groups: fresh group, MLT-treated (MC) no-MLT-treated (NC) group. After vitrification warming, viable from these three groups assessed for mitochondrial function, ultrastructure, permeability...
Chronic rejection that leads to diffuse narrowing and occlusion of graft vessels is the most important cause morbidity mortality following cardiac transplantation. The role underlying mechanism high-mobility group box 1 (HMGB1), as an established inflammatory mediator in acute rejection, remains poorly understood chronic rejection. Here, we assessed effects mechanisms HMGB1 on using single MHC Class II-mismatched mouse transplantation model. It was found increased accompanying with...
To evaluate whether melatonin (MT) supplementation during in vitro maturation (IVM) of human oocytes can reverse the age-related decline oocyte quality. We enrolled 172 patients aged ≥35 years (older reproductive-aged women) and 83 <35 (young who underwent fertilization between 2019 2022. conducted IVM with without 10 μM MT immature different ages. Oocyte embryo development were observed using a stereomicroscope. assessed immunofluorescence intensity mitochondrial function, measured copy...
Summary To explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using corresponding concentration PVP immobilization. The main outcome measures analyzed. Results indicated that, with decrease in concentrations, all increased. In particular, high-quality cleavage...
Abstract Deoxynivalenol (DON) is one of the most prevalent Fusarium mycotoxins, which cause detrimental effects on human and animal reproductive systems by inducing oxidative stress. Increasing evidence has suggested potential roles melatonin in protecting granulosa cells from injury, but underlying mechanisms remain largely elusive. Here, we demonstrated that suppression FOXO1 endoplasmic reticulum (ER) stress was engaged melatonin-mediated protection against damage upon DON exposure vitro....
Preimplantation embryonic lethality is a rare cause of primary female infertility. It has been reported that variants in the transducin-like enhancer split 6 ( TLE6 ) gene can lead to preimplantation lethality. However, incidence patients with not fully understood. In this study, we identified four carrying novel biallelic cohort 28 by whole-exome sequencing and bioinformatics analysis, accounting for 14.29% (4/28) cohort. Immunofluorescence showed levels oocytes from were much lower than...
Melatonin (MT) can improve the effect of cryopreservation on oocytes by suppressing oxidative stress and maintaining permeability oolemma. In this study, MT was firstly applied to human oocytes’ explore prolonged developmental competence its role. Collected in vitro-matured were cryopreserved MT-containing or MT-free medium for 0 6 months; after warming, viable assessed viability, intracellular protein expression, mitochondrial function, oxidation-antioxidant system. Meanwhile, fresh set as...
High-temperatures in the cockpit environment can adversely influence pilot behavior and performance.To investigate impact of high thermal environments on Chinese performance a simulated environment.Ten subjects volunteered to participate tests under 40°C 45°C high-temperature simulations an environmentally controlled chamber. Measures such as grip strength, perception, dexterity, somatic sense reaction, analytical reasoning were taken. The results compared Combined Index Heat Stress...
NK cells participate in the development of human multiple sclerosis (MS) and mouse experimental autoimmune encephalomyelitis (EAE), but roles different cell subsets disease onset remain poorly understood. In this study, murine were divided into CD27 high low/− subsets. The subset was decreased increased lymphoid organs during pre-onset stage EAE. Compared with counterpart naïve mice, showed lower expression Ly49D, Ly49H NKG2D, less production IFN-γ, whereas similar above mentioned surface...
Cardiac allograft vasculopathy (CAV) charactered with aberrant remodeling and fibrosis usually leads to the loss of graft after heart transplantation. Our previous work has reported that extracellular high-mobility group box 1 (HMGB1) participated in CAV progression via promoting inflammatory cells infiltration immune damage. The aim this study was investigate involvement HMGB1 pathogenesis CAV/fibrosis potential mechanisms using a chronic cardiac rejection model mice. We found high levels...
To explore whether embryo culture with melatonin (MT) can improve the embryonic development and clinical outcome of patients repeated cycles after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) failure, immature oocytes from controlled ovarian superovulation were collected for maturation (IVM) ICSI. The obtained embryos cultured 0, 10-11, 10-9, 10-7 10-5 M MT medium respectively, 10-9 was screened out as optimal concentration. Subsequently, 140 who underwent failed...
Is it possible to establish a new in-vitro activation (IVA) protocol for infertility treatment?A IVA procedure is an efficient and easily performed approach treatment of patients with diminished ovarian reserve (DOR).IVA primordial follicles or without stimulators has been developed treat primary insufficiency (POI) successfully. However, the efficiency still very low. There requirement optimize increased clinical application.Newborn mouse ovaries were used 1-h mechanistic target rapamycin...
Background High mobility group box protein 1 (HMGB1) is considered as a kind of sterile inflammatory mediators, which an overexpression in patients with unexplained recurrent spontaneous abortion (URSA). Specific targeting effect aspirin on HMGB1 has been revealed. Our previous studies have explored the application therapeutic target URSA disease mice model and human, but dynamic process downregulating concentration not demonstrated. Methods From December 2018 to November 2020, women ( n =...