A5051894963- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Prenatal Screening and Diagnostics
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Mitochondrial Function and Pathology
- Sperm and Testicular Function
- Hypothalamic control of reproductive hormones
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- Circular RNAs in diseases
- Pregnancy and preeclampsia studies
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Hearing, Cochlea, Tinnitus, Genetics
- Tissue Engineering and Regenerative Medicine
Peking University
2016-2025
Peking University Third Hospital
2016-2025
Ministry of Education of the People's Republic of China
2012-2025
National Clinical Research Center for Digestive Diseases
2023-2025
National Clinical Research
2024
Hainan University
2024
Nanjing Medical University
2023-2024
Beijing Obstetrics and Gynecology Hospital
2013-2024
Second Affiliated Hospital of Nanjing Medical University
2024
First Affiliated Hospital of Heilongjiang University of Chinese Medicine
2024
Abstract Human blastocysts are comprised of the first three cell lineages embryo: trophectoderm, epiblast and primitive endoderm, all which essential for early development organ formation. However, due to ethical concerns restricted access human blastocysts, a comprehensive understanding embryogenesis is still lacking. To bridge this knowledge gap, reliable model system that recapitulates stages needed. Here we developed three-dimensional (3D), two-step induction protocol generating...
Regenerative capacity declines throughout evolution and with age. In this study, we asked whether metabolic programs underlying regenerative capability might be conserved across species, if so, such drivers harnessed to promote tissue repair. To end, conducted metabolomic analyses in two vertebrate organ regeneration models: the axolotl limb blastema antler stem cells. further reveal why young individuals have higher than elderly, also constructed profiles for primate juvenile aged tissues,...
// Jinjin Lu 1 , Xiufen Zheng 2 Fan Li Yang Yu 3 Zhong Chen Liu Zhihua Wang Hua Xu Weimin Department of Urology, Tongji Hospital, Medical College, Huazhong University Science and Technology, Wuhan, 430030, P. R. China Pathology, Oncology, Surgery, Western University, Lawson Health Research Institute, London, Ontario, N6A 5A5, Canada Ultrasound, Correspondence to: Li, email: fanli117@hotmail.com Keywords: tunneling nanotubes, mitochondria, bladder cancer, cell invasion, connection Received:...
Abstract Context: Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder accompanied by chronic low-grade inflammation, but the molecular mechanism remains unclear. Objective: We investigated action of WNT5a in development inflammation PCOS and related signaling pathways. Design Setting: This was prospective study conducted at Division Reproduction Center, Peking University Third Hospital. Patients: A total 35 patients 87 control women who reported to clinic for vitro...
Aging is a major risk factor for many diseases, especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019 (COVID-19). Resolving cellular molecular mechanisms associated with aging higher mammals therefore urgently needed. Here, we created young old non-human primate single-nucleus/cell transcriptomic atlases of lung, heart artery, the top tissues targeted by SARS-CoV-2. Analysis cell type-specific aging-associated transcriptional...
Mitochondrial diseases are maternally inherited heterogeneous disorders that primarily caused by mitochondrial DNA (mtDNA) mutations. Depending on the ratio of mutant to wild-type mtDNA, known as heteroplasmy, defects can result in a wide spectrum clinical manifestations. Mitochondria-targeted endonucleases provide an alternative avenue for treating via targeted destruction mtDNA and induction heteroplasmic shifting. Here, we generated disease patient-specific induced pluripotent stem cells...
Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in number of genes including SOD1 FUS are associated familial ALS. Here we report the generation induced pluripotent stem cells (iPSCs) from fibroblasts ALS patients bearing SOD1+/A272C FUS+/G1566A mutations, respectively. We further generated gene corrected iPSCs using CRISPR/Cas9 system. Genome-wide RNA sequencing (RNA-seq) analysis motor...
Background: Despite recent advances that have improved the pregnancy success rates can be achieved via in vitro fertilization (IVF) therapy, it is not yet clear which blastocyst morphological parameters best predict outcomes of single transfer. In addition, most previous studies did exclude effect embryo aneuploidy on blastocysts Thus, present study investigated predictive value various transfer frozen euploid blastocysts. Methods: The retrospectively analyzed 914 cycles were performed at...
Mammals have limited regenerative capacity, whereas some vertebrates, like fish and salamanders, are able to regenerate their organs efficiently. The regeneration in these species depends on cell dedifferentiation followed by proliferation. We generate a mouse model that enables the inducible expression of four Yamanaka factors (Oct-3/4, Sox2, Klf4, c-Myc, or 4F) specifically hepatocytes. Transient vivo 4F induces partial reprogramming adult hepatocytes progenitor state concomitantly...
The third and fourth weeks of gestation in primates are marked by several developmental milestones, including gastrulation the formation organ primordia. However, our understanding this period is limited due to restricted access vivo embryos. To address gap, we developed an embedded 3D culture system that allows for extended ex utero cynomolgus monkey embryos up 25 days post-fertilization. Morphological, histological, single-cell RNA-sequencing analyses demonstrate cultured largely...
Abstract Oxidative stress plays a crucial role in organ aging and related diseases, yet the endogenous regulators involved remain largely unknown. This work highlights importance of metabolic homeostasis protecting against oxidative large intestine. By developing low-input user-friendly pipeline for simultaneous profiling five distinct cysteine (Cys) states, including free SH, total Cys oxidation (Sto), sulfenic acid (SOH), S -nitrosylation (SNO), -glutathionylation (SSG), we shed light on...
Maternal obese environment has been reported to induce oxidative stress and meiotic defects in oocytes, however the underlying molecular mechanism remains unclear. Here, using mice fed a high fat diet (HFD) as an obesity model, we first detected enhanced reactive oxygen species (ROS) content reduced Sirt3 expression HFD oocytes. We further observed that specific depletion of control oocytes elevates ROS levels while overexpression attenuates production with significant suppression spindle...