A5100371221- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Selenium in Biological Systems
- Kruppel-like factors research
- Circular RNAs in diseases
- Fibroblast Growth Factor Research
- Cancer Genomics and Diagnostics
- Food composition and properties
- RNA modifications and cancer
- Trace Elements in Health
- Cancer, Hypoxia, and Metabolism
- Reproductive System and Pregnancy
- Air Quality and Health Impacts
- Single-cell and spatial transcriptomics
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Bone Metabolism and Diseases
- Proteins in Food Systems
- Genomic variations and chromosomal abnormalities
- 3D Printing in Biomedical Research
- Vitamin K Research Studies
- Toxic Organic Pollutants Impact
- Pregnancy and preeclampsia studies
Qilu Hospital of Shandong University
2020-2024
Tsinghua University
2019-2024
Beijing Tsinghua Chang Gung Hospital
2024
Fujian Medical University
2022-2023
China University of Geosciences
2023
First Affiliated Hospital of Fujian Medical University
2023
State Key Laboratory of Food Science and Technology
2022
Jiangnan University
2022
Tianjin International Joint Academy of Biomedicine
2022
Nankai University
2022
Excessive mechanical stress is closely associated with cell death in various conditions. Exposure of chondrocytes to excessive loading leads a catabolic response as well exaggerated death. Ferroptosis recently identified form during aging and degeneration. However, it's potential association remains be illustrated.To identify whether can cause ferroptosis. To explore the role overloading chondrocyte ferroptosis.Chondrocytes were collected from unloading zones cartilage patients...
Vitamin K2 likely exerts its protective effects during osteoporosis by promoting osteoblast differentiation and mineralization. However, the precise mechanism remains to be fully elucidated. Autophagy maintains cell homeostasis breaking down eliminating damaged proteins organelles. Increasing evidence in recent years has implicated autophagy development of osteoporosis. The aim present study was verify whether vitamin (VK2) can induce mineralization osteoblasts. In study, MC3T3‑E1...
Abstract Pre-eclampsia is a pregnancy-related disease that may cause maternal, neonatal and fetal morbidity mortality exists in 3–5% of pregnancies worldwide. The discovery dysregulated microRNAs their roles placental development has provided new avenue for elucidating the mechanism involved this pregnancy-specific disorder. Here, human miR-181a-5p, microRNA increased both plasma placenta severe pre-eclamptic patients, invasion migration trophoblasts were investigated. Ectopic-expression...
Neural stem cells in the postnatal telencephalic ventricular-subventricular zone (V-SVZ) generate new interneurons, which migrate tangentially through rostral migratory stream (RMS) into olfactory bulb (OB). The Sp8 and Sp9 transcription factors are expressed neuroblasts, as well immature mature interneurons V-SVZ-RMS-OB system. Here we show that coordinately regulate OB interneuron development: although null mutants no major defect, conditional deletion of both resulted a much more severe...
Background: Long noncoding RNAs have been proven to play an important role in the progression of Alzheimer’s disease (AD). However, function small nucleolar RNA host gene 1 (SNHG1) AD remains be studied. Objective: To explore SNHG1 and clarify its potential mechanism. Methods: Amyloid β-protein (Aβ) was used construct cell model vitro. The expression levels miR-361-3p were determined by quantitative real-time polymerase chain reaction. Cell viability apoptosis measured counting kit 8 assay...
Aim: To examine the expression status of long-noncoding RNA LINC00265 and mechanistically elucidate its involvements in colorectal cancer (CRC). Methods: Relative abundances LINC00265, miR-216b-5p, tripartite-motif (TRIM)44 transcript were determined with real-time polymerase chain reaction. Cell viability was measured cell counting kit-8 kit. Glucose uptake, pyruvate, lactate production quantified commercially available kits. The potential regulatory effects miR-216b-5p on both TRIM44...
Abstract Tamoxifen-based endocrine therapy remains a major adjuvant for estrogen receptor (ER)-positive breast cancer (BC). However, many patients develop tamoxifen resistance, which results in recurrence and poor prognosis. Herein, we show that fatty acid oxidation (FAO) was activated tamoxifen-resistant (TamR) ER-positive BC cells by performing bioinformatic functional studies. We also reveal CPT1A, the rate-limiting enzyme of FAO, significantly overexpressed its enzymatic activity...
Purpose: Alzheimer’s disease is the most common neurodegenerative disease, characterized by accumulation of amyloid β peptides. MicroRNAs have been identified as significant regulators and therapeutic targets disease. However, roles miR-16-5p miR-19b-3p their mechanisms in progression remain largely unknown. Materials methods: Amyloid β-treated SH-SY5Y cells were used to study vitro . Transfection was conducted into using Lipofectamine 2000. The expression levels miR-16-5p, beta-site...
The multifunctional CaMKII has been implicated in vascular smooth muscle cell (VSMC) migration, but little is known regarding its downstream targets that mediate migration. Here, we examined whether regulates migration through modulation of matrix metalloproteinase 9 (MMP9). Using CaMKIIδ(-/-) mice as a model system, evaluated and MMP9 regulation vitro vivo. After ligation the common carotid artery, was activated neointima determined by oxidation autophosphorylation. We found robustly...
Abstract miRNAs are short, noncoding RNAs that regulate expression of target genes at post-transcriptional levels and function in many important cellular processes, including differentiation, proliferation, etc. In this study, we observed down-regulation miR-199a-5p during monocyte/macrophage differentiation HL-60 THP-1 cells, as well human CD34+ HSPCs. This resulted from the up-regulation PU.1 was demonstrated to transcription miR-199a-2 gene negatively. Overexpression by mimic transfection...
Non‑small cell lung cancer (NSCLC) is a leading subtype of cancer, with high mortality rates. Recently, long non‑coding RNAs (lncRNAs) have been associated NSCLC. The present study aimed to examine the role TP73 antisense RNA 1 (TP73‑AS1) lncRNA in TP73‑AS1 and microRNA(miR)‑34a‑5p expression levels were measured using reverse transcription‑quantitative PCR (RT‑qPCR) chromogenic situ hybridization (CISH). Cell proliferation, apoptosis, migration invasion was determined Counting Kit‑8...
Four new sesquiterpene lactones, (1 S,5 R,6 S,7 S,8 R,9 R,10 S,11 S)-6-acetoxy-9-hydroxy-4-oxo-pseudoguai-2(3)-en-12,8-olide, S,2 R,5 R,8 S,10 R)-6-acetoxy-2-ethoxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, R)-6-acetoxy-2-hydroxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, and 14-acetoxy-1 β,5 α,7 αH-4 β-hydroxy-guai-9(10),11(13)-dien-12,8 α-olide, along with 26 known were isolated from the whole plants of Inula hookeri C. B. Clarke. Their structures established based on spectroscopic methods...
The effective non-invasive diagnosis and prognosis are critical for cancer treatment. plasma cell-free DNA (cfDNA) provides a good material liquid biopsy its worth in this field is increasingly explored. Here we describe new pipeline effectively finding cfDNA-based biomarkers cancers by combining SALP-seq machine learning. Using the pipeline, 30 cfDNA samples from 26 esophageal (ESCA) patients 4 healthy people were analyzed as an example. As result, 103 epigenetic markers (including 54...
This study examined the effects of fucoidan on epithelial-to-mesenchymal transition (EMT) in a human triple-negative breast cancer (TNBC) cell line hypoxic microenvironment. Transwell and wound-healing assays were performed to analyze invasion migration MDA-MB-231 mammary cells, respectively. The expression levels EMT markers hypoxia-inducible factor-1α (HIF-1α) detected through western blotting. Under hypoxia, treatment inhibited proliferation cells. Fucoidan also suppressed Western...
Osteoporosis is a major public health problem that associated with high morbidity and mortality, its prevalence increasing as the world’s population ages. Therefore, understanding molecular basis of disease becoming priority. In this regard, studies have shown an imbalance in adipogenic osteogenic differentiation bone marrow mesenchymal stem cells (MSCs) osteoporosis. study, we conducted Weighted Gene Co-Expression Network Analysis to identify gene modules MSCs. Ontology Kyoto Encyclopedia...
Abstract Background MiRNAs play important roles in the development of ovarian cancer, activation primitive follicles, follicular development, oocyte maturation and ovulation. In present study, we investigated specific role miR-23a cov434 cells. Results Downregulation was observed serum PCOS patients compared with healthy control, suggesting inhibitory effect PCOS. MiR-23a positively correlated Body Mass Index (BMI) negatively Luteinizing hormone (LH), Testostrone (T), Glucose (Glu) Insulin...
MicroRNA (miRNA/miR)-409-5p has been reported to be implicated in prostate and breast cancers; however, its functional role ovarian cancer (OC) remains unclear. Therefore the aim of present study was investigate clinical significance biological function miR-409-5p OC. Here, reverse transcription-quantitative PCR analysis performed detect expression OC tissues cell lines. The association between clinicopathological characteristics patients with assessed using Fisher's exact test. Furthermore,...
The Nagengkangqie'er (Nageng) silver deposit, situated in the Eastern Kunlun Orogenic Belt (EKOB), Northwestern China. It contains 5,070 tons of contained at a grade 325 g/t. Nonetheless, genesis Nageng deposit is still under debate, due to absence definitive constraints mineralization age and metal sources. U-Pb data analysis post-ore calcite vein established minimum 207 ± 3 Ma, whereas maximum 217 4 Ma was determined from zircon obtained altered mineralized rhyolitic porphyry host-rock....
Long non-coding RNAs (lncRNAs) are a class of with unique characteristics. These RNA can regulate cancer cells' survival, proliferation, invasion, metastasis, and angiogenesis potential diagnostic prognostic markers. We identified seven-lncRNA signature related to the overall survival (OS) patients Ewing's sarcoma (EWS).We used an expression profile from Gene Expression Omnibus (GEO) database as training cohort screen out OS-associated lncRNAs in EWS further established using univariate Cox...
Gastric cancer (GC) is considered as one of the most widespread malignancies. Emerging evidence has shown that lncRNAs can function important oncogenes or tumor suppressors during GC progression.To investigate effect and mechanism lncRNA susceptibility 20 (CASC20) in proliferation metastasis cells.Data mining clinical samples were used to evaluate expression CASC20 adjacent tissues. was down-regulated cells by short-interfering RNA. Cell evaluated CCK-8 assay, cell migration invasion...
Cell free DNA (cfDNA) in human plasma carries abundant information, which has therefore been the key sample for noninvasive prenatal testing (NIPT) and liquid biopsy. Especially by using rapidly developed next-generation sequencing (NGS) techniques, genetic epigenetic information embedded cfDNA effectively extensively decoded. In this process, a step is to construct NGS library. Due its high degradation, single strand-based method was reported be more qualified library of cfDNA. order...