A5101739783- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Sesquiterpenes and Asteraceae Studies
- Plant Toxicity and Pharmacological Properties
- Salmonella and Campylobacter epidemiology
- Phytochemical compounds biological activities
- Biological and pharmacological studies of plants
- Botany and Plant Ecology Studies
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Probiotics and Fermented Foods
- Bioactive natural compounds
- Traditional and Medicinal Uses of Annonaceae
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Electrochemical Analysis and Applications
- Plant and animal studies
- Iron oxide chemistry and applications
- Ginseng Biological Effects and Applications
- Bioinformatics and Genomic Networks
- Traditional Chinese Medicine Analysis
- Alkaloids: synthesis and pharmacology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Magnolia and Illicium research
South China Agricultural University
2024
Oregon Health & Science University
2024
Chinese Academy of Agricultural Sciences
2022
Institute of Plant Protection
2022
Changzhou University
2022
Shanghai Jiao Tong University
2011-2020
Shanghai Normal University
2019-2020
Tianjin Institute of Industrial Biotechnology
2012
Chinese Academy of Sciences
2012
Peking University
2011
Cell wall catabolism during fruit ripening is under complex control and key for quality shelf life. To examine the role of abscisic acid (ABA) in tomato (Solanum lycopersicum) ripening, we suppressed SlNCED1, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a enzyme biosynthesis ABA. suppress SlNCED1 specifically fruits, thus avoid pleiotropic phenotypes associated with ABA deficiency, used an RNA interference construct driven by fruit-specific E8 promoter. accumulation transcript...
The two-component system PhoP-PhoQ is highly conserved in bacteria and regulates virulence response to various signals for within the mammalian host. Here, we demonstrate that PhoP could be acetylated by Pat deacetylated deacetylase CobB enzymatically vitro vivo Salmonella Typhimurium. Specifically, lysine residue 201(K201) winged helix–turn–helix motif at C-terminal DNA-binding domain of acetylated, its acetylation level decreases dramatically when encounter low magnesium, acid stress or...
Salmonella causes a range of diseases in different hosts, including enterocolitis and systemic infection. Lysine acetylation regulates many eukaryotic cellular processes, but its function bacteria is largely unexplored. The acetyltransferase Pat NAD+-dependent deacetylase CobB are involved the reversible protein Typhimurium. Here, we used cell animal models to evaluate virulence pat cobB deletion mutants S. Typhimurium found that critical for bacterial intestinal colonization Next,...
The ability to acetylate lysine residues is conserved across organisms, and acetylation of plays important roles in various cellular functions. Maintaining intracellular pH homeostasis crucial for the survival enteric bacteria acidic gastric tract. It has been shown that eukaryotes can stabilize by histone deacetylation. However, it remains unknown whether utilize a reversible protein system adapt an environment. Here we demonstrate acetylation/deacetylation critical Salmonella enterica...
Accumulating evidence indicates that bacterial metabolism plays an important role in virulence. Acetyl phosphate (AcP), the high-energy intermediate of phosphotransacetylase-acetate kinase pathway, is major acetyl donor E. coli. PhoP essential transcription factor for Here, we show Salmonella typhimurium non-enzymatically acetylated by AcP, which modifies its transcriptional activity, demonstrating acetylation Lysine 102 (K102) dependent on intracellular AcP. The level K102 decreases under...
In this study, we report the discovery of unexpected mechanistic intricacies Baeyer–Villiger monooxygenases (BVMOs) and provide insights that promise to help in extending their applications synthetic organic chemistry biotechnology. The basic mechanism BVMOs as catalysts oxidation unsymmetrical ketones R1–(C═O)–R2 is well known, which involves intermediacy short-lived Criegee intermediates. tendency R1 or R2 migrate preferentially breakdown intermediate follows traditional requirement an...
N휀-lysine acetylation is an abundant and important Post-translational modification in bacteria. We used the bacterial two-hybrid system to screen genome library of Salmonella Typhimurium identify potential proteins involved acetyltransferase Pat - or deacetylase CobB-mediated acetylation. Then, vitro (de)acetylation assays were validate targets, such as STM14_1074, NrdF, RhaR. Lrp, a leucine-responsive regulatory protein global regulator, was shown interact with Pat. further demonstrate that...
Four new sesquiterpene lactones, (1 S,5 R,6 S,7 S,8 R,9 R,10 S,11 S)-6-acetoxy-9-hydroxy-4-oxo-pseudoguai-2(3)-en-12,8-olide, S,2 R,5 R,8 S,10 R)-6-acetoxy-2-ethoxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, R)-6-acetoxy-2-hydroxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, and 14-acetoxy-1 β,5 α,7 αH-4 β-hydroxy-guai-9(10),11(13)-dien-12,8 α-olide, along with 26 known were isolated from the whole plants of Inula hookeri C. B. Clarke. Their structures established based on spectroscopic methods...
The PhoP-PhoQ two-component regulation system of Salmonella enterica serovar Typhimurium is involved in the response to various environmental stresses and essential for bacterial virulence. Our previous studies showed that acetylation plays an important role regulating activity PhoP, which consequently mediates change virulence S . Typhimurium.
One new unusual sesterterpenoid (<bold>1</bold>), four sesquiterpene dimers (<bold>2–5</bold>) together with nine known sesquiterpenes (<bold>6–14</bold>) were isolated from the aerial parts of <italic>Inula britannica</italic>.
Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress poorly understood.We used RNA sequencing explore acid-responsive sRNAs S. Typhimurium.It identified that 6S encoded ssrS was significantly upregulated at pH 3.0. The knockout strain showed a reduced ability survive Additionally, genes pathogenicity island-1 were downregulated strain....