A5065019668- Acne and Rosacea Treatments and Effects
- melanin and skin pigmentation
- Skin Protection and Aging
- Dermatology and Skin Diseases
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Pharmacological Effects of Natural Compounds
- Nonmelanoma Skin Cancer Studies
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- Nail Diseases and Treatments
- SARS-CoV-2 detection and testing
- Sepsis Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Infectious Diseases and Mycology
- Metabolism, Diabetes, and Cancer
- CRISPR and Genetic Engineering
Shanghai East Hospital
2016-2025
First Affiliated Hospital of Kunming Medical University
2014-2025
Kunming Medical University
2014-2025
Sichuan Agricultural University
2024-2025
Institute of Geographic Sciences and Natural Resources Research
2024
Chinese Academy of Sciences
2024
Jinyintan Hospital
2018-2023
Huazhong University of Science and Technology
2023
Shanghai University of Traditional Chinese Medicine
2023
Shanxi Agricultural University
2022
The outbreak of SARS-CoV-2 is posing serious global public health problems. Facing the emergence this pandemic, we established a portable microfluidic immunoassay system for easy-to-use, sensitive, rapid (<15 min), multiple, and on-site detection IgG/IgM/Antigen simultaneously. This integrated method was successfully applied detecting IgM IgG antibodies in clinical human serum as well antigen pharyngeal swabs from 26 patients with COVID-19 infection 28 uninfected people. assay demonstrated...
The two-component system PhoP-PhoQ is highly conserved in bacteria and regulates virulence response to various signals for within the mammalian host. Here, we demonstrate that PhoP could be acetylated by Pat deacetylated deacetylase CobB enzymatically vitro vivo Salmonella Typhimurium. Specifically, lysine residue 201(K201) winged helix–turn–helix motif at C-terminal DNA-binding domain of acetylated, its acetylation level decreases dramatically when encounter low magnesium, acid stress or...
Enterohemorrhagic Escherichia coli (EHEC) is one major type of contagious and foodborne pathogens. The VI secretion system (T6SS) has been shown to be involved in the bacterial pathogenicity bacteria-bacteria competition. Here, we show that EHEC could secrete a novel effector KatN, Mn-containing catalase, T6SS-dependent manner. Expression katN promoted by RpoS OxyR repressed H-NS, contributes growth under oxidative stress vitro. KatN secreted into host cell cytosol after phagocytized...
Background Atherosclerotic cardiovascular disease (ASCVD) remains the leading cause of death and disability both in U.S. worldwide. Antioxidants have been proved critical mitigating development atherosclerosis. This study aimed to investigate associations between composite dietary antioxidant index (CDAI) estimated 10-year ASCVD risk among adults. Methods Data extracted from National Health Nutrition Examination Survey were analyzed. A total 10,984 adults aged 18 years above included this...
Highly efficient capture and enrichment is always the key for rapid analysis of airborne pathogens. Herein we report a simple microfluidic device which capable fast bacteria enrichment. The was validated with Escherichia coli (E. coli) Mycobacterium smegmatis. results showed that efficiency can reach close to 100% in 9 min. Compared traditional sediment method, there also great improvement limit. In addition, various flow rate channel lengths have been investigated obtain optimized...
Data are limited on the impact of neuraminidase inhibitor (NAI) treatment avian influenza A(H7N9) virus RNA shedding.In this multicenter, retrospective study, data were collected from adults hospitalized with infection during 2013-2017 in China. We compared clinical features and shedding among patients different NAI doses combination therapies evaluated factors associated shedding, using Cox proportional hazards regression.Among 478 patients, median age was 56 years, 71% male, 37% died. The...
Abstract Iron dysregulation is associated with several diseases, including lung cancer, but the underlying mechanism yet unknown. directly binds CDK1, which upregulated in cancers, thereby promoting JAK1 phosphorylation and activation of STAT3 signaling to promote colorectal carcinogenesis. This study aimed investigate role iron/CDK1/STAT3 We found that iron-dependent CDK1 activity IL-6 receptor subunit GP130 post-transcriptionally via 4E-BP1, critical for JAK/STAT3 signaling. are essential...
Numerous studies have suggested a correlation between gut microbiota and acne vulgaris; however, no specific causal link has been explored. To investigate the possible relationship vulgaris, this study employed large-scale genome-wide association (GWAS) summary statistic. Initially, two-sample Mendelian randomization (MR) analysis was utilized to identify microflora responsible for vulgaris. We used Inverse Variance Weighted (IVW) method as main MR method. Additionally, we assessed...
Rapid analysis of airborne pathogens plays a critical role in early warning spreading infectious diseases, which is essential for public health and disease prevention. Herein we report an integrated microfluidic device that can perform pathogen capture, enrichment continuous-flow high-throughput gene analysis. The was validated by six frequently encountered bacteria shows great potential environmental protection.
Accumulating evidence indicates that bacterial metabolism plays an important role in virulence. Acetyl phosphate (AcP), the high-energy intermediate of phosphotransacetylase-acetate kinase pathway, is major acetyl donor E. coli. PhoP essential transcription factor for Here, we show Salmonella typhimurium non-enzymatically acetylated by AcP, which modifies its transcriptional activity, demonstrating acetylation Lysine 102 (K102) dependent on intracellular AcP. The level K102 decreases under...
Abstract Background The characteristics of neutralizing antibodies (NAbs) and antibody against major antigen proteins related to clinical outcomes in severe coronavirus disease 2019 (COVID-19) patients were still less known. Methods NAbs targeting nucleocapsid (N), spike protein (S), the receptor-binding domain (RBD) longitudinal plasma samples from LOTUS China trial measured by microneutralization assay enzyme-linked immunosorbent (ELISA). Viral load was determined real-time reverse...
Cutaneous squamous cell carcinoma (cSCC) is the second most frequent of keratinocyte-derived malignancies with actinic keratosis (AK) as a precancerous lesion. To comprehensively delineate underlying mechanisms for whole progression from normal skin to AK invasive cSCC, we performed single-cell RNA sequencing (scRNA-seq) acquire transcriptomes 138,982 cells 13 samples six patients including AK, in situ (SCCIS), and their matched tissues, covering comprehensive clinical courses cSCC. We...
Propionibacterium acnes (P. acnes) has been implicated in the progression of acne inflammation. Because current medications have various side effects, it is necessary to explore alternative possessing anti-inflammatory activity against P. acnes. We investigated inhibitory effects polyphyllin I (PPI) on acnes-induced inflammation vitro. In this study, we examined PPI production inflammatory cytokines HaCaT keratinocytes treated with heat-killed These showed increased expression Toll-like...
BackgroundReal-time reverse transcription-PCR (rRT-PCR) has been the most effective and widely implemented diagnostic technology since beginning of COVID-19 pandemic. However, fuzzy rRT-PCR readouts with high Ct values are frequently encountered, resulting in uncertainty diagnosis.MethodsA Specific Enhancer for PCR-amplified Nucleic Acid (SENA) was developed based on Cas12a trans-cleavage activity, which is specifically triggered by amplicons SARS-CoV-2 Orf1ab (O) N fragments. SENA first...
BackgroundShotgun metagenomics has been used clinically for diagnosing infectious diseases. However, most technical assessments have limited to individual sets of reference standards, experimental workflows, and laboratories.MethodsA panel performance metrics were designed examine the shotgun at 17 laboratories in a coordinated collaborative study. We comprehensively assessed reliability, key determinants, reproducibility, quantitative potential.FindingsAssay varied significantly across...
Sepsis is a common life-threatening disease in the intensive care unit (ICU) that usually treated empirically without pathogen identification. As non-invasive and high-throughput technology, plasma microbial cell-free DNA (mcfDNA) sequencing can detect unknown pathogens independent of previous clinical or laboratory information. In this study, total 199 cases suspected bloodstream infection (BSI) from January 2020 to June were collected, potential detected by simultaneous blood culture...