A5101703005- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Poxvirus research and outbreaks
- CRISPR and Genetic Engineering
- Virology and Viral Diseases
- Advanced biosensing and bioanalysis techniques
- Reproductive tract infections research
- Autism Spectrum Disorder Research
- Viral Infections and Vectors
- interferon and immune responses
- Bacillus and Francisella bacterial research
- Long-Term Effects of COVID-19
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- Parkinson's Disease and Spinal Disorders
- Kruppel-like factors research
Shenzhen Center for Disease Control and Prevention
2016-2025
National Institute for Viral Disease Control and Prevention
2024-2025
Beijing Tongren Hospital
2025
Capital Medical University
2025
Tianjin University
2024
Capital Institute of Pediatrics
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Haikou City People's Hospital
2017
Central South University
2017
University of East Anglia
2016
In 2007 the Centers for Disease Control and Prevention (CDC) reported a higher prevalence of autism spectrum disorder (ASD) in New Jersey, one wealthiest states United States, than other surveillance regions.To examine association socioeconomic status (SES) with ASD prevalence.Information on eight-year-olds from four counties was abstracted school medical records. US Census 2000 provided population median household income data.586 children were identified: 10.2/1000, boys girls (16 vs....
Accurate and early diagnosis of monkeypox virus (MPXV) is crucial for controlling epidemics treating affected individuals promptly. This study aimed to assess the analytical clinical performance MolecisionTM Monkeypox Virus qPCR Assay, Biorain ddPCR MAGLUMI® Ag (chemiluminescence immunoassay, CLIA) Assay. Additionally, it compare application antigen nucleic acid assays offer insights into using commercial assay kits. Specimens from 117 patients, serial diluted cell culture supernatant,...
We collected paired sera from live poultry workers in southern China and identified a high risk of asymptomatic influenza A(H7N9) virus infection, particularly females those with longer exposure to poultry. Background. Confirmed cases avian infection humans continue occur mainland China. Few confirmed have occurred despite potentially higher rates exposure. Methods. A serological survey was conducted May December 2013 market workers, March September the general population. Blood samples were...
Abstract The first local mpox outbreak in Guangdong Province, China occurred June 2023. However, epidemiological data have failed to quickly identify the source and transmission of outbreak. Here, phylogeny molecular evolution 10 monkeypox virus (MPXV) genome sequences from were characterized, revealing silent transmissions that may whose outbreaks suggested a correlation with Portugal several regions during same period. lineage IIb C.1, which includes all MPXV Guangdong, shows consistent...
BackgroundReal-time reverse transcription-PCR (rRT-PCR) has been the most effective and widely implemented diagnostic technology since beginning of COVID-19 pandemic. However, fuzzy rRT-PCR readouts with high Ct values are frequently encountered, resulting in uncertainty diagnosis.MethodsA Specific Enhancer for PCR-amplified Nucleic Acid (SENA) was developed based on Cas12a trans-cleavage activity, which is specifically triggered by amplicons SARS-CoV-2 Orf1ab (O) N fragments. SENA first...
In this paper we describe a series of 3-cyano-5-aryl-7-aminopyrazolo[1,5-a]pyrimidine hits identified by kinase-focused subset screening as starting points for the structure-based design conformationally constrained 6-acetamido-indole inhibitors CK2. The synthesis, SAR, and effects novel on Akt signaling cell proliferation in vitro are described.
Abstract The outer membrane (OM) of Gram-negative bacteria is a unique asymmetric lipid bilayer in which the leaflet composed lipopolysaccharide (LPS) and inner formed by glycerophospholipid (GPL). OM plays fundamental role protecting from harsh environments toxic compounds. transport assembly pathways for phospholipids bacterial are unknown. Cardiolipin (CL) an important biogenesis pathogenesis, (IM) protein PbgA, containing five transmembrane domains globular domain periplasm has been...
Aims: The screening and diagnosis of dengue virus infection play a crucial role in controlling the epidemic fever, highlighting urgent need for highly sensitive, simple, rapid laboratory testing method. This study aims to assess clinical performance MAGLUMI Denv NS1 detecting antigen. Methods: A retrospective was conducted sensitivity specificity using residual samples. Dengue-confirmed excluded samples, validated by qPCR, were subjected with accordance manufacturer’s instructions. linear...
High baseline autism spectrum disorder prevalence estimates in New Jersey led to a follow-up surveillance. The objectives were determine the year 2006 and identify changes of or characteristics children with disorder, between 2002 2006. cohorts included 30,570 children, born 1998 28,936 1994, residing Hudson, Union, Ocean counties, Jersey. Point by sex, ethnicity, subtype, previous diagnosis determined. For 2006, total 533 identified, consistent 17.4 per 1000 (95% confidence interval =...
The spread of influenza A/H3N2 variants possessing the hemagglutinin 121 K mutation and unexpectedly high incidence in 2017–2018 northern hemisphere season have raised serious concerns about next pandemic. We summarized national surveillance data seasonal China identified marked differences epidemics between southern China, particularly predominating subtype presence an additional summer peak China. Notably, a minor spring caused by different virus was also observed. revealed that 3C.2a...
A newly emerged H7N9 influenza virus poses high risk to human beings. However, the pathogenic mechanism of remains unclear. The temporal response primary alveolar adenocarcinoma epithelial cells (A549) infected with and H1N1 (H1N1, pdm09) were evaluated using proteomics approaches (2D-DIGE combined MALDI-TOF-MS/MS) at 24, 48 72 hours post infection (hpi). There 11, 12 33 proteins significant different expressions (P<0.05) 72hpi, especially F-actin-capping protein subunit alpha-1 (CAPZA1),...
Abstract Coronavirus disease 2019 (COVID-19) has spread rapidly worldwide, causing significant mortality. There is a mechanistic relationship between intracellular coronavirus replication and deregulated autophagosome–lysosome system. We performed transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients identified the aberrant upregulation genes in lysosome pathway. further determined capability two circulating markers, namely microtubule-associated...
Sporadic human infections with the highly pathogenic avian influenza (HPAI) A (H5N6) virus have been reported in different provinces China since April 2014. From June 2015 to January 2016, routine live poultry market (LPM) surveillance was conducted Shenzhen, Guangdong Province. H5N6 viruses were not detected until November 2015. The virus-positive rate increased markedly beginning December 2015, and LPMs all districts of city. Coincidently, two cases histories exposure developed symptoms...
Abstract Background SARS-CoV-2 is a newly emerged coronavirus, causing the coronavirus disease 2019 (COVID-19) outbreak in December, 2019. As drugs and vaccines of COVID-19 remain development, accurate virus detection plays crucial role current public health crisis. Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) kits have been reliably used for RNA since beginning outbreak, whereas isothermal nucleic acid amplification-based point-of-care automated also...
Purpose: To investigate and characterize the putative Elizabethkingia anophelis contaminant isolated from throat anal swab samples of patients three fever epidemic clusters, which were not COVID-19 related, in Shenzhen, China, during pandemic. Methods: Bacteria cultured ( n = 28) 3) 28 adolescent patients. The bacterial strains identified using matrix-assisted laser desorption/ionization time flight mass spectrometry (MALDI-TOF/MS) VITEK2 automated identification system. Nucleic acids...
Gastric cancer presents as a complex solid tumor and is the third leading cause of global cancer‑associated mortality. The genetic alterations in gastric remain unclear deserve further investigation. Mining Cancer Genome Atlas adenocarcinoma dataset identified frequent loss zinc finger BTB domain containing 7A (ZBTB7A) gene locus significant correlation between low ZBTB7A expression poor patient survival. belongs to POZ/BTB Kruppel transcription factor family. In present study,...
Chlamydia psittaci is a small bacterium often found in birds, including poultry, and domesticated mammals, which causes psittacosis (or parrot fever) humans. Different strains of C. respond variably to antibiotics, suggesting possible risk antibiotic resistance. In general, different genotypes have relatively stable hosts pathogenicity.Macrogenomic sequencing was performed using nucleic acids extracted from patients' alveolar lavage fluid samples analyzed for genetic variability resistance...
Avian influenza A (H7N9) virus has caused several epidemics and infection in both human poultry. With mutation, the H7N9 gained its fifth endemic China. Early diagnosis is crucial for control of viral spread poultry prognosis infected patients. In this study, we developed evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay rapid detection hemagglutinin neuraminidase gene H7N9. Our H7-LFD-RPA N9-LFD-RPA were able to detect 32 fg nucleic acid which more...
Moraxella catarrhalis (M. catarrhalis) was an underestimated respiratory infection pathogen that has been largely overlooked. The limited availability of rapid and sensitive detection methodologies hindered M. diagnostic in clinical settings contributed to its underestimation. To address this issue, we devised two recombinase polymerase amplification (RPA)-based assays for rapid, reliable catarrhalis, termed catarrhalis-RPA-Flu catarrhalis-RPA-LFB, which utilized fluorescence...
Summary COVID-19 is caused by the SARS-CoV-2 coronavirus and was first reported in central China December 2019. Extensive molecular surveillance Guangdong, China’s most populous province, during early 2020 resulted 1,388 RNA positive cases from 1.6 million tests. In order to understand epidemiology genetic diversity of we generated 53 genomes infected individuals Guangdong using a combination metagenomic sequencing tiling amplicon approaches. Combined epidemiological phylogenetic analyses...