A5027728636- RNA modifications and cancer
- Enzyme Catalysis and Immobilization
- interferon and immune responses
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- COVID-19 Clinical Research Studies
- Microbial Metabolic Engineering and Bioproduction
- DNA and Nucleic Acid Chemistry
- Tuberculosis Research and Epidemiology
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Tryptophan and brain disorders
- vaccines and immunoinformatics approaches
- Biochemical and Molecular Research
- Enzyme Production and Characterization
- Heat shock proteins research
- NF-κB Signaling Pathways
- Inflammasome and immune disorders
Huashan Hospital
2018-2025
Fudan University
2016-2025
State Key Laboratory of Genetic Engineering
2015-2024
Guangxi Medical University
2019-2024
First Affiliated Hospital of GuangXi Medical University
2019-2024
Henan University
2023
Pudong Medical Center
2023
Beijing YouAn Hospital
2023
Capital Medical University
2023
Henan Provincial People's Hospital
2023
A rapid and sensitive method is crucial for nucleic acid detection. Recently, RNA-guided CRISPR/Cas12a nuclease-based methods present great promise In the methods, however, DNA amplification subsequent Cas12a cleavage separated whole process takes as long 2 h. Most importantly, uncapping operation increases risk of aerosol contamination. this study, we propose a CRISPR/Cas12a-based named "Cas12aVDet" By integrating recombinase polymerase (RPA) with in single reaction system, detection can be...
Significance The protein gasdermin D (GSDMD) is the physiological substrate of inflammatory caspases and plays key roles in cell pyroptosis upon microbial infection associated danger signals. GSDMD, as well other members, can bind lipid form pore structures to induce pyroptosis. However, detailed structural information for GSDMD remains unknown. We have determined crystal structure C-terminal domain human GSDMD. reveals that first loop inserts into N-terminal help stabilize full-length...
In addition to storage of genetic information, DNA can also catalyze various reactions. RNA-cleaving DNAzymes are the catalytic DNAs discovered earliest, and they cleave RNAs in a sequence-specific manner. Owing their great potential medical therapeutics, virus control, gene silencing for disease treatments, have been extensively studied; however, mechanistic understandings substrate recognition catalysis remain elusive. Here, we report three form 8-17 DNAzyme crystal structures. adopts...
Abstract Gasdermin-E (GSDME), the executioner of pyroptosis when cleaved by caspase 3, plays a crucial role in tumor defense and response to chemotherapy drugs cells. So far, there are poorly known mechanisms for expression regulation GSDME during cell death. Here, we identify transcription factor Sp1 (Specificity protein 1) as positive regulator GSDME-mediated pyroptosis. directly interacts with promoter at −36 ~ −28 site promotes gene transcription. Further, knockdown or inhibition...
Abstract The conversion of serine and glycine that is accomplished by hydroxymethyltransferase 2 (SHMT2) in mitochondria significantly upregulated various cancers to support cancer cell proliferation. In this study, we observed SHMT2 acetylated at K95 colorectal (CRC) cells. SIRT3, the major deacetylase mitochondria, responsible for deacetylation. SHMT2-K95-Ac disrupts its functional tetramer structure inhibits enzymatic activity. also promotes degradation via K63-ubiquitin–lysosome pathway...
Integrated satellite-terrestrial networks (ISTNs) are one of the new research directions for future 5G networks. Satellite coverage enables communication throughout most world. However, security concerns associated with inherent broadcast nature satellites rarely considered. As an emerging paradigm in terrestrial communication, physical layer (PLS) could have potential applications ISTN. This article describes challenges applying PLS to ISTN, including correlated channels, co-channel...
Significance Aminoacyl-tRNA synthetases (AARSs) comprise a family of 20 enzymes that catalyze the first step protein synthesis by esterifying specific amino acids to 3′ ends their cognate tRNAs. Protein acetylation, an evolutionarily conserved posttranslational modification, has recently been extensively investigated regulate diverse cellular processes. This work provides molecular evidence one aminoacyl-tRNA is modulated reversible acetylation. study not only reveals regulatory mechanism...
Metal-mediated base pairs have been extensively utilized in many research fields, including genetic-code extension, novel therapeutics development, and nanodevice design. Compared to other cations, AgI is more flexible pairing with natural pairs. Herein, we present a DNA structure containing two C-AgI -C the first reported G-AgI -G pair short 8mer strand. This not only provides detailed insight into these -mediated base-pairing patterns DNA, but also represents nonhelical driven by...
The compact CRISPR/Cas9 system, which can be delivered with their gRNA and a full-length promoter for expression by single adeno-associated virus (AAV), is promising platform therapeutic applications. We previously identified SauriCas9 that displays high activity requires simple NNGG PAM, but the specificity moderate. Here, we three Cas9 orthologs, Staphylococcus lugdunensis (SlugCas9), lutrae (SlutrCas9) haemolyticus (ShaCas9), mammalian genome editing. Of these SlugCas9 recognizes PAM...
Abstract Receptor interacting protein kinase 3 (RIP3 or RIPK3), the critical executor of cell programmed necrosis, plays essential roles in maintaining immune responses and appropriate tissue homeostasis. Although E3 ligases CHIP PELI1 are reported to promote RIP3 degradation, however, how post-translational modification regulates activity stability is poorly understood. Here, we identify tripartite motif TRIM25 as a negative regulator RIP3-dependent necrosis. directly interacts with through...
Abstract Ultra-stable fibrous structure is a hallmark of amyloids. In contrast to canonical disease-related amyloids, emerging research indicates that significant number cellular termed ‘functional amyloids’, contribute signal transduction as temporal signaling hubs in humans. However, it unclear how these functional amyloids are effectively disassembled terminate transduction. RHIM motif-containing the largest amyloid family discovered thus far, play an important role mediating necroptosis...
Parkinson's disease (PD), one of the most devastating neurodegenerative brain disorders, is characterized by progressive loss dopaminergic neurons in substantia nigra (SN) and deposits α-synuclein aggregates. Currently, pharmacological interventions for PD remain inadequate. The cell necroptosis executor protein MLKL (Mixed-lineage kinase domain-like) involved various diseases, including inflammatory bowel diseases; however, its precise role remains unclear. Here, we investigated...
One-quarter of the world's population is infected with Mycobacterium tuberculosis (Mtb). After initial exposure, more immune-competent persons develop asymptomatic latent infection (LTBI) but not active diseases, creates an extensive reservoir at risk developing tuberculosis. Previously, we constructed a novel recombinant Sendai virus (SeV)-vectored vaccine encoding two dominant antigens Mtb, which elicited immune protection against acute Mtb infection. In this study, nine latency-associated...
Accumulating evidence indicates that bacterial metabolism plays an important role in virulence. Acetyl phosphate (AcP), the high-energy intermediate of phosphotransacetylase-acetate kinase pathway, is major acetyl donor E. coli. PhoP essential transcription factor for Here, we show Salmonella typhimurium non-enzymatically acetylated by AcP, which modifies its transcriptional activity, demonstrating acetylation Lysine 102 (K102) dependent on intracellular AcP. The level K102 decreases under...
The aim of the present study was to investigate potential prognostic value members heat shock protein (HSP)90 family in non-small cell lung cancer (NSCLC) patients. mRNA expression profiles 1,926 NSCLC patients, which available from Kaplan-Meier plotter database, were included study. High HSP90AA1 significantly associated with a poorer rate overall survival (OS) for all patients [hazard ratio (HR), 1.21; 95% confidence interval (CI): 1.06-1.37; P=0.004], as well adenocarcinoma (ADE; HR, 1.3;...