A5101535462- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Advanced Sensor and Energy Harvesting Materials
- Hydrogels: synthesis, properties, applications
- Biochemical and Structural Characterization
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Photocatalysis Techniques
- Genomics and Phylogenetic Studies
- Immune cells in cancer
- Plant tissue culture and regeneration
- Legal and Policy Analysis in Indonesia
- Mosquito-borne diseases and control
- Microbial Community Ecology and Physiology
- Cancer Research and Treatments
- Education and Learning Interventions
- Electrochemical Analysis and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Biosensors and Analytical Detection
- Nanoplatforms for cancer theranostics
- Carbon and Quantum Dots Applications
- Insect symbiosis and bacterial influences
- Glycosylation and Glycoproteins Research
- Porphyrin and Phthalocyanine Chemistry
Anyang Institute of Technology
2018-2025
Fudan University
2012-2025
Zhongshan Hospital
2012-2025
Pudong Medical Center
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
Xiyuan Hospital
2022-2025
Xinjiang Medical University
2025
First Affiliated Hospital of Zhengzhou University
2024
Shenzhen Institutes of Advanced Technology
2024
Chinese Academy of Sciences
2024
The development of natural polymer-based hydrogels, combining outstanding injectability, self-healing, and tissue adhesion, with mechanical performance, able to facilitate full-thickness skin wound healing, remains challenging. We have developed an injectable micellar hydrogel (AF127/HA-ADH/OHA-Dop) adhesive self-healing properties accelerate healing. Dopamine-functionalized oxidized hyaluronic acid (OHA-Dop), adipic dihydrazide-modified HA (HA-ADH), aldehyde-terminated Pluronic F127 (AF127)...
The high fat and sucrose diet, known as the obesogenic diet (OD), has been related to low-grade chronic inflammation neurodevelopmental disorders. Emerging evidence suggests that OD influences cognitive social function via gut-brain axis. However, effects of during adolescence on future health have unclear. Meanwhile, underlying mechanisms effective interventions are not fully understood. Polysaccharides, one most abundant substances in
A large number of SpCas9 orthologs has been computationally identified, but their genome editing potential remains largely unknown. In this study, a GFP-activation assay was used to screen panel 18 orthologs, ten which demonstrated activity in human cells. Notably, these had preference for purine-rich PAM sequences. Four the tested displayed enhanced specificity compared SpCas9. Of particular interest is SeqCas9, recognizes simple NNG and displays comparable SpCas9-HF1. addition, SeqCas9...
The compact CRISPR/Cas9 system, which can be delivered with their gRNA and a full-length promoter for expression by single adeno-associated virus (AAV), is promising platform therapeutic applications. We previously identified SauriCas9 that displays high activity requires simple NNGG PAM, but the specificity moderate. Here, we three Cas9 orthologs, Staphylococcus lugdunensis (SlugCas9), lutrae (SlutrCas9) haemolyticus (ShaCas9), mammalian genome editing. Of these SlugCas9 recognizes PAM...
Almost all current electrochemiluminescent reagents require real-time electrochemical stimulation to emit light. Here, we report a novel reagent, nitrogen-deficient graphitic carbon nitride (CNx), that can afterglow electrochemiluminescence (ECL) after cessation of electric excitation. CNx obtained by post-thermal treatment (CN) with KSCN has cyanamide group and nitrogen vacancy, which created defects trap electrically injected electrons. The trapped electrons slowly release react...
Dielectric relaxation behaviors of three types thermally sensitive poly(N-isopropylacrylamide) (PNIPAM) microgels with different cross-linking density distributions were investigated in a frequency range from 40 Hz to 110 MHz at temperatures 15 °C 55 °C. After eliminating the electrode polarization low frequency, two remarkable relaxations observed, one kHz and other range. The low-frequency is attributed counterion whole measuring temperature range, while high-frequency probably dominated...
[Figure: see text].
The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its targeting scope limited by the protospacer-adjacent motif (PAM). To expand target scope, it crucial to develop CRISPR toolbox capable of recognizing multiple PAMs. Here, using GFP-activation assay, we tested activities 29 type II-C orthologs closely related Nme1Cas9, 25 which are active in human cells. These recognize diverse PAMs with variable length and nucleotide preference, including purine-rich,...
Abstract Due to the tolerance of mismatches between gRNA and targeting sequence, base editors frequently induce unwanted Cas9-dependent off-target mutations. Here, develop models predict such off-targets, we design gRNA-off- target pairs for adenine (ABEs) cytosine (CBEs) stably integrate them into human cells. After five days editing, obtain valid efficiency datasets 54,663 55,727 off-targets ABEs CBEs, respectively. We use train deep learning models, resulting in ABEdeepoff CBEdeepoff,...
The installation of deuterium atoms at distal C(sp3)–H bonds is a longstanding synthetic challenge. Through the synergistic combination chain-walking strategy and transfer hydrodeuteration, we present remote hydrodeuteration unactivated alkenes sites, aided by native directing groups, using readily available pinacolborane (HBpin) D2O as H– D+ sources, respectively. Significantly, this tactic offers complementary pattern to conventional methods that adduct H–D, or its surrogate, unsaturated...
Necrotizing enterocolitis (NEC) is a leading cause of premature newborn morbidity and mortality. The clinical features NEC consistently include prematurity, gut dysbiosis enteral inflammation, yet the pathogenesis remains obscure. Herein we combine metagenomics targeted metabolomics, with functional in vivo vitro assessment, to define novel molecular mechanism NEC. One thousand six hundred forty seven publicly available datasets were analyzed (NEC = 245; healthy 1,402) using artificial...
CjCas9 is one of the smallest CRISPR-associated (Cas9) nucleases for mammalian genome editing. However, it requires a long N
Introduction Acute ischemic stroke (AIS) poses a significant risk to human health. Intravenous thrombolysis and mechanical thrombectomy are essential treatments for AIS, offering substantial benefits neurological recovery brain protection. However, their efficacy is often limited by stringent time constraints contraindications, restricting accessibility certain patient populations. Investigating novel therapeutic strategies is, therefore, crucial. Our team developed Xiongzhitongluo granules...
Drug-target binding affinity (DTA) prediction is vital in drug discovery and repositioning, more researchers are beginning to focus on this. Many effective methods have been proposed. However, some current certain shortcomings focusing important nodes molecular graphs dealing with complex structural molecules. In particular, when considering substructures molecules, they may not be able fully explore the potential relationships between different parts. addition, protein structures, ignore...
<title>Abstract</title> <bold>Background </bold>Latent tuberculosis infection (LTBI) is a significant reservoir foractive (TB) development. Identifying key risk factors for LTBI crucialfor effective prevention and control strategies. Machine learning (ML) techniques can uncover complex relationships between disease outcomes. <bold>Methods </bold>Data were collected from the "Tuberculosis Management Information System" in China. was defined by positive tuberculin skin tests. Four ML...
Chromosomes of species exhibit a variety high-dimensional organizational features, and chromatin loops, which are fundamental structures in the three-dimensional (3D) structure genome. Chromatin loops visible speckled patterns on Hi-C contact matrix generated by chromosome conformation capture methods. The play an important role gene expression, predicting during whole genome interactions is crucial for deeper understanding 3D function. Here, we propose CGLoop, deep learning based neural...