A5101523953- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Orthopedic Surgery and Rehabilitation
- Elbow and Forearm Trauma Treatment
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Cardiomyopathy and Myosin Studies
- Shoulder Injury and Treatment
- Hip disorders and treatments
- Hip and Femur Fractures
- Bone and Joint Diseases
- Sarcoma Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Bone fractures and treatments
- Bone Tumor Diagnosis and Treatments
- Advanced biosensing and bioanalysis techniques
- Bone health and treatments
- RNA Research and Splicing
- Bone Metabolism and Diseases
- Orthopaedic implants and arthroplasty
- Protease and Inhibitor Mechanisms
- RNA Interference and Gene Delivery
Second Affiliated Hospital of Inner Mongolia Medical University
2015-2025
Chinese Academy of Sciences
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2025
Suzhou University of Science and Technology
2024-2025
Shenzhen Institutes of Advanced Technology
2025
Hebei Normal University
2024
Guangxi University of Chinese Medicine
2022-2024
Guangxi University
2022-2024
Hunan Normal University
2024
Chinese PLA General Hospital
2018-2023
Abstract Background and Aims Lackluster results from recently completed gene therapy clinical trials of VEGF-A delivered by viral vectors have heightened the need to develop alternative delivery strategies. This study aims demonstrate pre-clinical efficacy safety extracellular vesicles (EVs) loaded with mRNA for treatment ischaemic vascular disease. Methods After encapsulation full-length into fibroblast-derived EVs via cellular nanoporation (CNP), collected were mouse models injury. Target...
Acquisition of drug-resistant phenotypes is often associated with chemotherapy in osteosarcoma. Studies show that high-mobility group box 1 (HMGB1) plays an important role facilitating autophagy and promotes drug resistance osteosarcoma cells. In this study, we determined the targeting miR-22 to HMGB1 regulation on HMGB1-mediated cell proliferation, migration, invasion Results demonstrated well paired 3'-UTR downregulated expression blocked during cells vitro. Further study showed blockage...
Introduction: Abnormal status of gene expression plays an important role in tumorigenesis, progression and metastasis breast cancer.Mechanisms silence or activation were varied.Methylation genes may contribute to alteration expression.This study aimed identify differentially expressed hub which be regulated by DNA methylation evaluate their prognostic value cancer bioinformatic analysis.Methods: GEO2R was used obtain microarray data from GSE54002, GSE65194 GSE20713, GSE32393.Differentially...
Apoptosis of osteoblasts, triggered by prolonged or excessive use glucocorticoids (GCs), has been identified as a dominant contributor to the development osteoporosis and osteonecrosis. However, molecular mechanisms underlying GC‑induced apoptosis are multifaceted remain be fully elucidated. The present study aimed explore correlation between dexamethasone (DEX)‑induced reactive oxygen species (ROS), autophagy in MC3T3‑E1 osteoblast‑like cells. Cell viability was assessed using Counting...
Osteoarthritis (OA) is the most prevalent musculoskeletal disease, imposing a significant public health burden. Exosomes might be an effective means of treating OA. To investigate role exosomes from adipose tissue-derived stromal cells (ADSCs) in We explored whether ADSCs could absorbed by OA chondrocytes, there were differences miR-429 expression and ADSC exosomal enhance chondrocyte proliferation to exert therapeutic effects Controlled laboratory study. isolated cultured 4-week-old...
Six new highly oxygenated polycyclic cyathane-xylosides, named striatoids A–F (1–6), were isolated from the cultures of basidiomycete Cyathus striatus. Their structures established by comprehensive spectroscopic analysis including 2D NMR (HMBC, HSQC, ROESY, 1H–1H–COSY) and HRESIMS experiments. Compounds 2 3 possess an unusual 15,4′-ether ring system. The compounds dose-dependently enhanced nerve growth factor (NGF)-mediated neurite outgrowth in rat pheochromocytoma (PC-12) cells.
Colorectal cancer (CRC) is a major cause of global mortality. Gene expression profiles can help predict prognosis patients with CRC. In most previous studies, disease recurrence was analyzed as the survival endpoint. Thus we aim to build robust gene signature for prediction overall (OS) in Fresh frozen CRC tissues from 64 were using Affymetrix HG-U133plus 2.0 arrays. By performing univariate analysis, 6487 genes found be associated OS our cohort. KEGG analysis revealed that these mainly...
Abstract Muscle LIM protein (MLP, CSRP3 ) is a key regulator of striated muscle function, and its mutations can lead to both hypertrophic cardiomyopathy (HCM) dilated (DCM) in patients. However, due lack human models, mechanisms underlining the pathogenesis MLP defects remain unclear. In this study, we generated knockout MLP/CSRP3 embryonic stem cell (hESC) H9 line using CRISPR/Cas9 mediated gene disruption. disruption had no impact on cardiac differentiation cells led confirmed deficiency...
Osteosarcoma (OS) is the most common primary malignant tumor of bone affecting children and adolescents. Chemotherapy now considered as a standard component OS treatment, not only for children, but also adults. However, chemoresistance continues to pose challenge therapy. Inhibition autophagy has been demonstrated decrease in OS. Moreover, microRNA‑22 (miR‑22) inhibits autophagy, leading an improvement sensitivity cisplatin (CDDP) The aim present study was therefore investigate whether...
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Ginsenoside Rg1 (Rg1) has been demonstrated to have antidiabetic and antiosteoporotic activities. The aim of this study was investigate the protective effect against diabetic osteoporosis underlying mechanism. In vitro , we found that increased number osteoprogenitors alleviated high glucose (HG) induced apoptosis by MTT assays flow cytometry. qRT‒PCR western blot analysis suggested can also promote secretion vascular endothelial growth factor (VEGF) coupling osteogenesis angiogenesis....
Doxorubicin (DOX) is widely used to treat various cancers affecting adults and children; however, its clinical application limited by cardiotoxicity. Previous studies have shown that children are more susceptible the cardiotoxic effects of DOX than adults, which may be related different maturity levels cardiomyocyte, but underlying mechanisms not fully understood. Moreover, researchers investigating DOX-induced cardiotoxicity caused human-induced pluripotent stem cell-derived cardiomyocytes...
MicroRNAs (miRNAs) play vital roles in biological activities, but their vivo imaging is still challenging due to the low abundance and lack of efficient fluorescent tools. RNA aptamers with high affinity background emerge for bioimaging yet suffering from brightness. We introduce a rational design based on target-mediated entropy-driven toehold exchange (EDTE) induce release aptamer subsequently light up corresponding fluorophore, which achieves selective miRNAs good stability both living...
Introduction Traumatic brain injury (TBI) is considered the most common traumatic neurological disease, associated with high mortality and long-term complications, a global public health issue. However, there has been little progress in serum markers for TBI research. Therefore, an urgent need biomarkers that can sufficiently function diagnosis evaluation. Methods Exosomal microRNA (ExomiR), stable circulating marker serum, aroused widespread interest among researchers. To explore level of...
Abstract Liver fibrosis, a critical precursor to cirrhosis and leading cause of mortality, highlights the urgent need for identification effective therapeutics. Activation hepatic stellate cells (HSCs) is key process in liver fibrosis. This study presents live‐cell drug‐screening approach that specifically targets fibrosis‐associated collagen type I alpha 1 ( COL1A1 ) mRNA activated HSCs through use fluorogenic RNA self‐assembly. It employs dual‐probe system construct an Mango II structure,...
Abstract Myomesin‐1 (encoded by MYOM1 gene) is expressed in almost all cross‐striated muscles, whose family (together with myomesin‐2 and myomesin‐3) helps to cross‐link adjacent myosin form the M‐line myofibrils. However, little known about its biological function, causal relationship mechanisms underlying MYOM1‐related myopathies (especially heart). Regrettably, there no MYMO1 knockout model for study so far. A better further understanding of biology urgently needed. Here, we used...
The Myosin light chain 3 (MYL3) gene encodes the ventricular essential isoform, which is an important modulator of sarcomeric myosin cross-bridge kinetics. Variants in MYL3 are a cause hypertrophic cardiomyopathy and dilated with cardiac failure sudden death (SCD). To further elucidate involvement pathogenesis cardiomyopathies, we have created knockout human embryonic stem cell line using CRISPR/Cas9 system. Notably, this MYL3-knockout retains normal morphology, pluripotency, karyotype. This...